65
New Brunswick Scientific
User’s Guide
6.
Remove the two O-rings installed in the pH port; first slide the white
Teflon O-ring onto the probe, then follow with the black 12mm port
adapter O-ring.
7.
Do not yet close up all the elements of the port adapter.
8.
Gently insert the probe into the appropriate port, allowing the O-rings to
seat fully into the port.
#
NOTE:
The fit may be snug. Gently rotate the probe as you press it into the port
to avoid breakage.
9.
Finger tighten the bottom portion of the port adapter into the port.
10.
Adjust the probe to the desired height; then, nesting the ferrules, close the
top portion of the adapter onto the bottom portion.
11.
Finger tighten the knurled adapter assembly.
CAUTION!
We recommend that you avoid the use of hydrochloric acid (HCl) with
the BioFlo/CelliGen 115 for pH control or any other purpose, because
HCl corrodes stainless steel. Over time, it will severely damage the
headplate, a costly component to replace, and other stainless steel
components.
Phosphoric and sulfuric (10% maximum concentration) acids are
acceptable and are commonly used for pH control.
7.2.2
pH Probe Maintenance & Storage
Check for any trapped air bubbles in the electrode’s tip to remove bubbles,
hold electrode upright and shake electrode gently.
The probe should be stored standing upright, and the electrode tip should
be immersed in the solution of 3 molar KCl or a buffer solution between
pH 4.00 and pH 7.00.
If the probe is so equipped, the two rubber T stoppers
should be inserted.
CAUTION!
Never let a pH probe rest on its tip, and never leave a pH probe in DI
water.
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