NEW BRUNSWICK SCIENTIFIC BioFlo CelliGen 115, Руководство по эксплуатации

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BioFlo/CelliGen 115
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M1369-0050 User’s Guide
For anaerobes, several strategies can be used to eliminate oxygen:
•
Reducing agents can be added to the media.
•
Vigorous agitation (normally used to increase dissolved oxygen in the media) is
not required. A low agitation rate, however, is required to keep the cells in
suspension and to provide mixing of the liquid to maintain good temperature
control. An inert gas such as nitrogen can be sparged into the media to provide the
necessary anaerobic conditions.
•
Additionally, a gas overlay can be installed to introduce the inert gas into the
headspace. The gas introduced via the gas overlay can come from splitting of the
sparge gas (by using a T or Y fitting).
For the growth of microaerophiles, a premixed gas is introduced into the sparge line
and overlay. The gas mixture is dependent on the particular organism that you are
culturing.
13.11 Harvesting Procedure
When the vessel is set up on the control cabinet, adjust the level probe’s tip to the level
at which you want harvesting to stop (i.e., below the current liquid level):
1. Assign a feed pump as
Lvl2 Wet
, to pump liquid out of the vessel.
2. Aseptically connect the feed pump’s tubing to the harvest port.
3. Turn the pump ON . Since the liquid is in contact with the probe, the circuit will
close, and the pump will begin pumping liquid out of the vessel.
4. When the liquid drops below the probe tip, the pump will stop.
See also Section 11.1,
Pump Assignment
. If you assign the pump to None instead of
Lvl2 Wet , it will harvest as much as possible.
13.12 Shutdown Procedure
At the end of a run, to shut down the system, follow these steps:
1. Set GasFlo to OFF .
2. Set Agit and Temp to OFF .
3. Set all other control loops to OFF .
4. Turn off the power.
5. If the system is not to be used for several days, disconnect the power plug.