NEW BRUNSWICK SCIENTIFIC BioFlo CelliGen 115, Руководство по эксплуатации

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BioFlo/CelliGen 115
y
M1369-0050 User’s Guide
1. Gently compress the baffle ring at its ends (to avoid scratching the vessel
walls). You may find it convenient to squeeze the tab with your thumb.
2. Slide the assembly inside, with the tab facing up, until it comes to rest at
the bottom of the vessel.
3. Orient the baffle so the opening is opposite the gradations on the vessel.
4.8.5 Install Impeller(s)
Install the impeller(s) as follows:
A.
For Cell Culture:
Slide the impeller onto the agitation drive shaft (from the
bearing housing). Position the impeller at least 10 mm above the sparger.
Clamp it down in place.
#
NOTE:
It is normal for the agitation impeller shaft to be very resistant to turning
by hand. The shaft seal resistance ensures sterile operation.
B.
For Fermentation:
Slide one impeller onto the agitation drive shaft (from the
bearing housing). Position this lower impeller according to the table below.
Clamp it down in place. Then install the second (upper) impeller in the same
manner.
Table 2: Impeller Positions
Distance from Bottom of Headplate to Top of Impeller Blade
1.3L 3.0L 7.5L 14.0L
Lower Impeller 4 1/8 in.
105 mm
6 11/16 in.
170 mm
8 7/8 in.
225 mm 12 in.
305 mm
Upper Impeller 2 5/8 in.
67 mm
4 in.
102 mm
6½ in.
165 mm
9¼ in.
235 mm
#
NOTE:
The distances indicated above provide a recommenced starting point. As
working volumes and agitation rates change, you may wish to adjust the
impeller location(s).
#
NOTE:
It is good practice to lightly lubricate all O-rings, port threads and adapter
threads with silicone grease* (NBS part number P0860-1050) before you
install equipment in the headplate. Also inspect the headplate O-ring to be
sure it is securely seated in its groove.
*For cell culture, you may want to use IPA or glycerol instead of silicone.