134
BioFlo/CelliGen 115
y
M1369-0050
User’s Guide
Now that the vessel is detached from the unit, it can be cleaned. Remove any
remaining cotton and foil covering the ports. The rubber sampler bulb should
be removed and rinsed separately. The glass wool can be removed at this
point, too. Detach the sampling tube and wash it separately. Open the valve
on the sampling port and all clamps on all tubing connected to ports for proper
washing (be sure to remove the media prior to unclamping any tubing below
liquid level, such as a side harvest line). The headplate should be detached by
loosening and then removing the clamps that hold it to the rest of the vessel.
Those clamps may require rinsing. The remaining culture broth should be
sterilized, or emptied into a bucket and disinfected by using bleach or other
accepted disinfectant prior to disposal. Note that some media may be
incompatible with this procedure, in which case the media can be placed into
another container for sterilization prior to disposal.
The headplate should be washed thoroughly with warm water and then with
deionized (DI) water. It may be necessary to scrub off any accumulations of
biomaterial. A pad that won't scratch the steel is required for this. The
agitation shaft, thermowell, harvest and sparger tubes, and the short beveled
tips of the interior portion of the base-type addition ports will often require
special attention. All tubes and shafts must be cleaned. Note that there may be
some residual base or acid left in those lines, so extreme caution and the use of
chemically-resistant gloves is highly recommended for this procedure. It is
often necessary to hand wipe surfaces with a paper towel in order to fully
remove residual traces of small particulate debris.
The washing of the bottom portion of the vessel requires the same procedures
as the headplate. Note that the sides of the vessel, particularly near the baffle,
may require special attention.
The vessel can now be cleaned by washing with detergent, or by using a
cleaning solution. If the vessel is to be sterilized, all standard precautions must
be taken. Note that for this purpose, the vessel does not need to be sealed
except for those previously cited valves and tubing which run under the liquid
level. It will be necessary to use water in the vessel. We recommend the use
of DI (deionized) water, and the fill should be at least as high as your standard
level for a run.
Unless you have already specifically wiped the residual grease off the top of
the glass cylinder, there should be enough so that the headplate can be clamped
to the glass vessel. DO NOT tighten the headplate clamps with the same force
used to install the headplate prior to a run, as this could lead to vessel damage.
Instead, the lightest possible pressure should be used.
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