Section 12- Protein Bradford
Step 2:
Measure
Standards
Up to 5 replicates of
each of up to 7
standards can be
measured.
The software will not
allow measurement
of samples until a
minimum of 1
standard and
references – or 2
standards – are
measured.
Polynomial curve
fitting requires more
standard points.
Step 3:
Measure
Samples
Sample
concentrations can
be calculated by
using linear
interpolation (point-
to-point) between
the two standards
flanking the
unknown sample
or
by using polynomial
fitting. [Note: In order
to obtain a
concentration value
(mg/ml) the sample
(unknown) must fall
within the limits of
the standard curve].
Standard Curve Features
Standard curves can be saved and reloaded for reference use by using the ‘Standard Curve’ pull down menu and
choosing the’ Save as’ or ‘Load Standard Curve’ functions. Selecting the ‘View Standard Curve’ button allows the user to
review the standard curve at any time. New to version 3.5.1 is the ability to reload a standard curve concentration series
using the ‘Load Standard Curve Set-up’.
Delete Standard Points
The Delete Point button allows the user to delete points by first selecting the data point to be deleted in the table and then
choosing the ‘Delete Point’ button. If erroneous or non-representative readings are encountered for a specific standard,
all replicates of that standard are cleared by selecting ‘Delete 1 Standard’. Additionally, all standards can be deleted at
once using the ‘Reset all Standards’ button.
12-3
