Molecular Devices SPECTRAmax 340PC, Руководство оператора

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Instrument Description
SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3
Introduction
General Overview
The SPECTRAmax
®
340PC incorporates a holographic grating monochromator
which allows you to specify a precise wavelength, from 340 nm to 850 nm, for
the absorbance maximum of your sample. The SPECTRAmax 340PC can mea-
sure optical density (OD) for a single point in time (endpoint), over a specified
period of time (kinetic), or over a selected wavelength range (spectral scan).
Typical applications include endpoint assays (ELISAs, performed in microplates
or as dot blots, quantitation of cytoproliferation by MTT reduction, colorimetric
protein assays, and kinetic measurements (enzyme studies, such as determination
of the activity of enzymes released from cells, and kinetic ELISAs).
Standard 96-well microplates, strip wells and filter-bottom microplates can be
used in the SPECTRAmax 340PC. The contents of the wells in a microplate can
be mixed automatically by shaking before each read cycle, which makes it possi-
ble to perform kinetic analysis of solid-phase, enzyme-mediated reactions (mixing
is not critical for liquid-phase reactions).
The temperature of the microplate chamber can also be regulated, if desired,
from 4°C above ambient to 45°C.
An on-board microprocessor calculates and reports the absorbance (optical den-
sity) or the Kinetic rate (change in optical density over time) for each well, allow-
ing the SPECTRAmax 340PC to function as a stand-alone system when
connected to an external printer.
The SPECTRAmax 340PC can also be controlled by an external computer.
SOFTmax
®
PRO software from Molecular Devices provides integrated instru-
ment control and statistical data analysis.