Molecular Devices SPECTRAmax 340PC, Руководство оператора

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Chapter 3
3-4 SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual
mark the blank wells on the template as shown in Figure 3.1. Templates can also
be constructed from microplates or from microplate lids with marked well loca-
tion. Fill selected microplate wells with an optically dense solution (OD > 0.500)
or blacken microplate lids at selected sites with a glassware marking pen.
Figure 3.1 : Preparing the Blank Pattern Template
When you have prepared the blank pattern, press the
[Read Mode]
key to scroll
through the list of modes until BLANK appears.
If the incubator is on and the drawer is closed, open the drawer by pressing the
[Drawer]
key. The drawer will remain open for approximately 10 seconds, after
which a beeping sound will alert you that the drawer is closing automatically.
Load the template in the drawer, matching template well A1 with drawer position
A1, making sure the template is flush on all sides and lies flat. Touch the
[READ]
key any time after the template is properly positioned in the drawer.
When reading is complete, the instrument will print a display of the well loca-
tions that have been designated
as members of the blank pattern. Blank wells will
be represented on the printout by the character “#.” All other wells will be indi-
cated by a single dot character (“.”).
The blank pattern may be stored as part of an assay protocol.
If a blank pattern has not been saved, it may be cleared by reading an empty
drawer in BLANK mode, or by recalling the factory default protocol. To change
or clear the blank pattern for an assay protocol, recall the protocol and tempo-
rarily switch the read mode to BLANK. To change the blank pattern, read the
new blanking template; to clear the blank pattern, read an empty drawer. To save
the new settings, switch back to the original read mode and press the
[Save]
key.
Endpoint Mode
Use the up or down arrows on the
[Read Mode]
key to scroll through the list
until you select Endpoint (shown in the LCD).