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sigmaaldrich.com

User Guide

DiluCult

™

and DiluCult

™

instruments

The life science business of

Merck operates as MilliporeSigma

in the US and Canada.

Содержание DiluCult

Страница 1: ...sigmaaldrich com User Guide DiluCult and DiluCult 2 instruments The life science business of Merck operates as MilliporeSigma in the US and Canada...

Страница 2: ...ers 3 Operator and Equipment Safety 3 System Components 5 Setting Up the Instrument 6 Unpacking the Instrument 6 Installing the Instrument 6 Operating the Instrument 11 Powering the Instrument On 11 E...

Страница 3: ...on Do not expose the DiluCult and DiluCult 2 instruments to liquid If liquid gets inside the instrument through the ventilation cut outs at the back of the instrument avoid any contact with the instru...

Страница 4: ...ion time for a 375 g sample diluted at 1 10 80 s Dispensing time for 90 mL 7 s 7 s Dispensing time for 225 mL 10 s 10 s Maximum number of pumps 2 integrated 2 integrated Dispensing distribution mode F...

Страница 5: ...acy sticker RS232 output USB output System Components Tubing holder Removable drip tray Fixed dispensing arm Nozzle holder Bag holder DiluCult Instrument Front Back On Off switch Identification sticke...

Страница 6: ...DiluCult 2 Instrument The shipping box contains the following One dispensing assembly 6 4 mm diameter for GL45 bottle per pump One set of 10 GeckoGrip One set of adhesive gum One monitoring software C...

Страница 7: ...t Instrument DiluCult 2 Instrument 2 1 Nozzle protector Installing the Bag Holder Clip the bag holder into the two holes in the base of the instrument For the DiluCult 2 instrument only also clip on t...

Страница 8: ...pump extension are not aligned They should be staggered in relation to each other 4 Clip the pump extension on the pump 5 Open the 2 covers If the alignment is incorrect separate the pump from the pum...

Страница 9: ...2 Install the tubing The direction of flow is indicated by the arrows on the housing 3 For tubing with an internal diameter smaller than 6 4 mm delivered as standard see Adjusting the Pump and Insert...

Страница 10: ...r is 6 4 mm To change the setting turn the button on each side of the pump to obtain the right setting Tubing is pinched Tubing is not pinched 2 Insert the tubing into the pump 3 Close the pump keepin...

Страница 11: ...dy to function The arm of the DiluCult 2 instrument is initialized Ensuring the Accuracy of the Weighing Platform To ensure greater accuracy of the weighing platform wait 10 minutes before the first u...

Страница 12: ...ocessing Green indicates that the weighing platform is stable Proceed with the process 11 Start the dilution by pressing the Go button The In progress LED lights This indicates that the dilution is ta...

Страница 13: ...Start the dispensing by pressing the Go button The In progress LED lights This indicates that the dispensing is taking place The light under the instrument turns orange and flashes slowly The robotic...

Страница 14: ...on instructions at the back of the unit button again The instrument beeps 3 times 7 Remove the weight The instrument is calibrated and ready for use Using the Monitoring Software For traceability the...

Страница 15: ...ed is a dilution Displayed as a whole number no decimal places the number N when the dilution ratio is 1 N Sample weight Displayed in grams with 2 decimal places Theoretical weight Displayed when the...

Страница 16: ...g holder Drip tray Fixed arm Nozzle holder including roller Protecting plate for nozzle Follow these maintenance practices Autoclave all the dispensing assemblies every day when working in sterile con...

Страница 17: ...ons correctly Cleaning the Nozzle Holder DiluCult 2 Instrument Only 1 Switch the instrument to standby mode by pressing the button 2 Press the Go button twice The arm initializes 3 Unscrew the nozzle...

Страница 18: ...sure that the pumps are set correctly see Adjusting the Pump and Inserting the Tubing Stretch the tubing on both sides without opening the pump The pump perforates the tubing Ensure that the tubing ha...

Страница 19: ...nsing reports Ensure that the DiluCult instrument is connected to the USB cable Disconnect and then reconnect the cable and ensure that the computer makes a sound every time If the computer does not h...

Страница 20: ...demarks of Merck KGaA Darmstadt Germany or its affiliates All other trademarks are the property of their respective owners Detailed information on trademarks is available via publicly accessible resou...