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Chapter 3: Image Management

View images in [Browse] panel, it displays the image File name,
capturing time, color depth (bit), picture resolution and image size.
It also allows you to

add comments to any individual image

. When

you view this image next time in the ISC, it will show the image
comment.

Image Management and Processing Shortcuts

ISC provides some quick functions on the right hand side of the
software in

Browse

or

Image

mode.

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Содержание HD1500MET

Страница 1: ...al number ISCapture 2 Software version number Instruction Manual 3 Description of the problem Screenshots of the Key to the Instruction Manual ISC is shortened name used for ISCapture Square brackets are used to indicate items such as menu names button names and window names that appear on the computer screen Help Refer to Help About ISCapture menu for software information and technical support Pr...

Страница 2: ...4 Gain Frame Speed Data Width 15 Levels 15 COLOR CONTROL 16 Flat Fielding Function 17 FLUORESCENCE MODE 18 Pseudo Color function 18 Black Level 19 Fluorescence Wizard 19 Temperature Control 20 PARAMETER 21 LIVE IMAGE MEASUREMENT 21 Measurement List 22 Edit Scale Line 23 Create Calibration File 24 Calibration Table 26 Measurement List 27 Measurement 27 Annotate 28 Ruler 28 Layer 29 LIVE IMAGE SHORT...

Страница 3: ...er Tucsen Camera Driver H Series Setup exe to the computer Double click on it to start the installation Follow the Next button to finish the installation 2 Go to the Device Manager and check whether the driver is installed properly If there is NO YELLOW FLAG with the camera under Imaging Device in Device Manager the driver is installed successfully see Figure 1 ...

Страница 4: ... ISCapture was installed in your PC the installer will automatically detect it and ask to remove it first before install the new one If the previous installed ISCapture is still running it will ask to close the software and then start the installation Start ISCapture 1 Connect the camera to the PC Be sure the driver is already installed properly 2 Double click the ISCapture shortcut to start the s...

Страница 5: ...1 Set Auto Exposure Observe the preview and adjust the microscope or lens to make image in focus Normally Auto Exposure function can get proper brightness preview If preview is still dark manually set Gain to the middle of the slider When get preview in focus set Gain back to the initial value change to ManualExposure mode and extend the exposure time manually until get proper brightness images 2 ...

Страница 6: ...ge resolution get different preview and capture images Click the Lock tag to lock unlock the preview and capture resolution Unlocking it allows to set different preview and capture resolution Usually use for low resolution preview high resolution for capture 4 Go to FileSave tab to set image save format directory and file name a Select UseFileSaveConfig to pre set the capture image format save dir...

Страница 7: ...eFileSaveDialog to use pop up dialog to set capture image file name save directory and format Every time click Capture button the file save dialog will pop up to ask to set file name save directory and image format ...

Страница 8: ...8 Chapter 2 Image Acquisition Capture tab allows to do parameter settings capture still images record videos and live image measurement ...

Страница 9: ...Basic Control Provide basic exposure setting white balance and image resolution settings Note Lock WB ONLY will appear when HD1500MET camera is attached NO DRIVER INSTALLATION IS NEEDED WHEN USE HD1500MET ...

Страница 10: ... can be seen after decoding the Bayer matrix Image Denoise checked to do the image noise reduction to save better quality image Note In long time exposure application it needs to wait quite a while to save an image Combined Measurement Checked to save the live measurements to the captured image In Use File Save Dialog mode a pop up file save configure window will appear when the Capture or Video b...

Страница 11: ...Use Recording checkbox to combine all the continuous shooted images into a avi format video file The created video file will be playback as the frame rate set in this dialog used by default If do not key in anything IS is Select Use Time stamped to name the image by the capture time automatically The time stamp file name will be in the form of MMDDHHmmSS Here MM indicates the month DD indicates th...

Страница 12: ...tart stop video recording Click Rec Config to get video recording configure window It provides Manual and Auto modes to stop the recording Manual mode Video button is pushed to start and stop the recording Auto mode pre set the number of frames or the time for videos and Video is pushed ISC will stop the recording automatically after save pre set number of frames or pre set time is up Rec Config C...

Страница 13: ... C Program Files ISCapture Note This default destination might NOT be allowed storing any files if the user is not logged in as Administrator in Windows Vista 7 or later Image may not save if the default path is used We recommend either to change the file save path or release this folder Write authority for other user accounts Exposure Control Change the Exposure time Gain to adjust the image brig...

Страница 14: ...are Lock will stop the auto exposure calculation While auto exposure is working it will keep on calculating the image brightness to get proper exposure time During this if you already see a good live image for you you can push to lock it Adjust the exposure time manually Two ways to change the exposure time Key in the exposure time in the edit box directly then click to confirm it Pull the slide b...

Страница 15: ...will help to get the new exposed image earlier If the exposure time is less than 2 3 seconds it is not necessary to use it Gain Frame Speed Data Width Levels The levels tool can move and stretch brightness levels in a histogram using three main components a black point white point and midtone slider Channel List This allows to choose whether to edit RGB channel or one of the three individual color...

Страница 16: ...the left brightens the image by stretching out the shadows and compressing the highlight whereas movement to the right performs the opposite Note Move the White Point Slider towards left it is able to reveal some information in dark area If move Black Point Slider towards right it will reveal bright area information It is also allowed to key in the image levels directly to do the adjustment After ...

Страница 17: ...tart the flat fielding parameter calculation and apply it to the live images When the check box is unchecked the calculated flat fielding parameter is NOT applied to the live images To get better flat fielding result Move the sample to a blank area first apply the flat fielding then move the sample back When the lighting is changed re do the FlatFielding to correct the uneven brightness ...

Страница 18: ...ble for CCD monochrome and Discovery MH15 cameras Apply pseudo color to the live images Two ways provided for color assignment a Click on the preferred color or move the slider to choose it b Assign the color according to the fluorescence dye in the drop down menu Dye Type ...

Страница 19: ...wait When searching for the imaging target at the beginning we recommend to set a shorter exposure time but make larger Gain and Black level first After you find the target then reduce the Gain and Black level and increase the exposure time This will aid in a better image acquisition Or you can follow the Fluorescent Wizard in the next section to get proper settings for low light applications Fluo...

Страница 20: ... image in this step Step2 When get bright enough images adjust your microscope or lens to make the image in focus Step3 Click Optimize to optimize the parameter settings set longer exposure time and reduce the gain value at the same time automatically to get low noise images Temperature Control This function is ONLY available for the CCD cameras assembled with temperature control chip ...

Страница 21: ... Click to open drop down menu choose the preferred parameter team and then push to make selected parameters take effect on the live image Delete function Choose a parameter team and click to remove it from the PC Backup function backup all the saved parameter teams Click to get a pop up dialog which allows to select the parameter setting information save directory Recover function Restore all the ...

Страница 22: ...22 Measurement List ...

Страница 23: ...23 Edit Scale Line Double click on the scale to get its properties and make changes to it Edit scale character Edit the frame of the scale Edit scale line length and name ...

Страница 24: ...our application it also requires you to take the calibration slide picture with the reduce lens attached Note If ONLY ONE objective and ONE resolution is used in the application one calibration slide picture is enough The calibration slide picture MUST be taken with exactly the same lens or microscope settings as the target image taken 2 Click to start to create calibration file 3 Click Load Image...

Страница 25: ...accurate result than using 1 scale unit 5 Enter the name for the calibration file and the length of the line you draw Note If you need more than one calibration file using objective reducing lens if it is used resolution as the name of the calibration file is recommended This can help to prevent using the wrong file to do the calibration Note When keying in the length please pay more attention to ...

Страница 26: ...e calibration The new calibration file named 10X is created in the Calibrate Table Calibration Table Click Calibrate Table to open the calibration table Select the correct calibration file for current image measurement Using the WRONG calibration file will make the measurement result innacurate Please make sure the calibration file is correctly corresponding to the current image Hence it is ...

Страница 27: ...T or Excel file Measurement ISC allows you to do line parallel perpendicular rectangle circle polygon arc and angle measurement The Count function allows you to manually count the objects And the Annotate function offers to add comments on the images Double click on the measure data to get the measurement configure window It allows you to change the measured data name color thickness background co...

Страница 28: ...t the comment change the background color and on off the annotate arrow Ruler Click on to show or cancel the cross line on the images The displayed ruler unit is determined by the selected calibration file Double click on the ruler to get the ruler property and change the ruler color The default color is BLACK ...

Страница 29: ...ts The layer function makes adding a large number of measurements on the processed image review simple and easy If you have already applied some measurements on the image the Layer function automatically creates Background and Layer01 for the current image ...

Страница 30: ... layers It allows you to choose any layers to view Checked Cur means the corresponding layer is displayed currently Select different Cur to switch between different layers In the Visible column the selected check box means all the measurements in the corresponding layers also display on the current layer Uncheck the check box and the corresponding measurement will be invisible in the current layer...

Страница 31: ...layer information in the text file The layer information will be saved as LayerInfo txt in Disk D by default Click Browse to find the existed layer information text file Click Load to load the layer information to the current image Live image shortcut On the right hand side of the live image window some shortcuts are provided to process the live image quickly ...

Страница 32: ...th bit picture resolution and image size It also allows you to add comments to any individual image When you view this image next time in the ISC it will show the image comment Image Management and Processing Shortcuts ISC provides some quick functions on the right hand side of the software in Browse or Image mode ...

Страница 33: ...4 Image Processing Tab In this section ISC provides advanced image processing functions and also allows you to do the measurement on the still images Image Processing Provide basic still image processing functions ...

Страница 34: ...o get the image histogram It allows you to adjust the image levels The level adjustment is the same as live image level adjustment Get more detail in Capture Exposure Control Levels Measure Click on Measure at the top of the ISC to get the measurement tools Then select the corresponding tools to measure the still images get more details in Capture Live Measurement ...

Страница 35: ...ation Step 2 Click on image thumbnails to add corresponding images The image position indicator shows the added images position Maximum 4 frame images are allowed to add for fluorescence combination Step 3 Click on one added image indicator to start applying color for it Click on one image indicator to select it The selected one will be in dark color unselected ones will be gray white Assign color...

Страница 36: ... 4 Click on Combine All to combine all the colored images Note Optimize checkbox is recommended to select during the combination It will optimize image background to get a better image If the optimize function is not selected the created image will keep all the original information No extra processing is applied to the image data Note After combining the fluorescence image Sharp function in Image ...

Страница 37: ...wrong color to selected image just click on the tiny cross beside each indicator to delete it If you want to cancel the current combination just click on Close All to cancel the combination Original images Combined image Label The label text will be displayed on the lower right corner of the ...

Страница 38: ...ill be saved as image file name _bak For example the original image file name is IS jpg then this image with a label will be saved as IS_bak jpg So the original image is still kept Class Counting Class counting function allows to do 5 different types samples counting manually Each type will be assigned with different color dots ...

Страница 39: ...ox Select the corresponding images and apply the function This function combines multiple images to create one focused panorama high dynamic range image Browse the image folder which you are going to do the processing All the images in the folder will be listed on the left hand side Click on one image or select multiple images the images will be highlighted in BLUE ...

Страница 40: ...g HDR to stack all the selected source images and get an processed image Note When selecting a wrong image just click on it then click Delete to remove it Delete all will remove all the selected images Meiji Techno America 5895 Rue Ferrari San Jose CA 95138 Tel 1 800 832 0060 Fax 408 226 0900 www meijitechno com info meijitechno com 100 Lauman Lane Suite A Hicksville NY 11801 Tel 877 877 7274 Fax ...

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