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Chapter 1 – Introduction
1.1 Product Overview
The Luna-FL™ automated cell counter is a small, fast, and affordable image-based cell counting
device, containing bright field and dual fluorescence optics, that automatically counts various kinds
of cells for research use.
The Luna-FL™ helps measure the number as well as the viability of cells (live, dead, total cells) with
sophisticated optical components and advanced image analysis algorithms. Due to the innovations
introduced by Logos Biosystems, the Luna-FL™ provides a state of the art cell counting device and
eliminates the tedium and subjectivity of manual cell counting.
The Luna-FL™ can be used in a very simple procedure. For example, in order to accomplish dual
fluorescence counting, first, mix 18
l of the cell sample with 2
l of the AO/PI (Acridine Orange and
Propidium Iodide) from the cell viability kit (in case that there is not enough cell sample, 9
l of the
cell sample with 1
l of the AO/PI solution can be used to load the total 10
l in the counting
chamber). Second, load 10-12
l of the mixed cell suspension into the Luna-FL™ cell counting slide.
Third, insert the slide into the slide port of the instrument and adjust the focus knob to get an
appropriate cell image. Last, press the “Count” button and the results of cell count and viability will
be displayed on the screen. The counting image can be downloaded onto a USB drive in a TIF
format for future analysis.
<AO/PI Stained Cell Image>
Assay principle
: AO stains all cells, and PI stains dead cells only. If AO and PI exist together, AO
fluorescence is mostly quenched by PI. Therefore, AO fluorescence (green) is found in live cells
only, and PI fluorescence (red) is found in dead cells only. Cell viability analysis using AO/PI double
staining is a proven and accepted method.
