6
General Guidelines for Using the Luna-FL™ Automated Cell Counter
In order to achieve the best results with the Luna-FL™ automated cell counter, users must follow
the instructions below carefully.
1. The instrument must be operated in compliance with the environmental conditions described on
page 7. In particular, the temperature and humidity conditions are important.
2. Samples must be handled in an appropriate way, depending on user’s requirements.
3. Hold the Luna-FL™ cell counting chamber slide by the edges to avoid touching its optical
surface. Make sure that no damage or contamination occurs on the optical surfaces of the slide.
4. After mixing the cell sample with the supplied reagents, perform cell counting within 1-3
minutes for accurate cell viability measurements. Optimally, count your sample at least twice
(duplicate readings) to obtain better results.
5. Since the Luna-FL™ is calibrated before shipping, users generally do not need to re-calibrate
before use. However, if re-calibration is needed, for example due to long-term use, please refer
to Section 7.3 Calibrating the Counter.
6. Do not touch trypan blue solution and other reagents with bare hands, as they are hazardous
chemicals. After using chamber slides, dispose of them as hazardous waste. Do not reuse the
slides.