66
(8
l), 2.00 (10
l), 2.50 (12
l), 3.33 (14
l), 5.00 (16
l)
to 10.00 (18
l).
Dilution factor corrects the actual number of cells; thus
the analyzed results are generated for the original
samples.
Dilution factor will help the users during repeated
counting of the samples of high cell density such as
fermented CHO cells.
Minimum
Cell Size
1-90
5
Use this parameter to set the minimum cell size for
inclusion. The unit is 1 micrometer.
Maximum
Cell Size
1~90
60
Use this parameter to set the maximum cell size for
inclusion. The unit is 1 micrometer.
Green
Fluorescence
Threshold
1~10
5
Green and Red Fluorescence Threshold will determine
the level of threshold during the image processing.
Increased threshold will detect less cells by subtracting
the background more stringently. Decreased threshold
can detect more cells, but will increase the number of
noise signal.
Red
Fluorescence
Threshold
1~10
5
6.3 Managing Protocols
To manage protocols, use the buttons described below.
Button
Description
Load
Use this button to load one of the saved protocols to apply for counting or
adjusting the parameters.
Edit
After selecting a protocol, parameters can be adjusted by pressing this button.
Arrow buttons located over and under the parameter will be activated. Press the
arrow buttons to change the values for each parameter.
When no protocol is selected, this button is inactivated.
If you “Load” a new protocol after you “Edit” a protocol, the changed parameters
will be automatically saved to the same protocol name.
Delete
After selecting one of the protocols on the screen, press this button to remove it