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8
Mounting Stage to Microscope
with Dovetail Substage
The following description is for mounting the
stage on to microscopes which have a circular
dovetail substage assembly.
Attach the curved stage clamps (part no. 9542) to
the base of the stage using the supplied hex
screws and the outer most holes in the base plate.
Adjust the two positioning screws so that approxi-
mately 5mm of thread is exposed on the inside
edge of the clamp. This will roughly position the
stage in the centre of the dovetail.
Place the stage onto the dovetail then focus a 10X
objective lens on the aperture of the silver block.
Using the two positioning screws ensure that the
aperture is in the centre of the field of view.
For other types microscope’s substage, see the
included diagram for fitting instruction.
Setting up the Condenser for Koehler
Illumination.
Place a small sample on a cover slip and place
onto the surface of the silver block. Use the 10X
lens to focus on the sample. Now close down the
microscope field diaphragm and adjust the con-
denser focus so that the edges of the diaphragm
are in focus. Now use the condenser positioning
screws to centre the condenser in your field of
view. Open the diaphragm so that it just fills the
field of view.
For more information about Koehler illumination
see the extremely informative ‘microscopy Pri-
mer’ on the Molecular Expressions website.
http://micro.magnet.fsu.edu/primer/
index.html
9542 curve clamps set
Dovetail substage
Содержание FDCS196
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