6. Instrument setup– Condenser CenterIng
6.4 Condenser centering
For the Leica DMi8 with tiltable transmitted light illumina-
tor arm
Set the Koehler illumination for your microscope (not avail-
able for S40 and S70 condensers).
The condenser is also pre-adjusted in the factory.
However, depending on how the condenser is disassembled
and reassembled, it may be necessary to re-center the con-
denser in some cases. Therefore, check the condenser cen-
tering.
• Adjust the image so it is as sharp as possible by
carefully moving the condenser up and down
(Fig. 58.2). Try this with different field diaphragm settings
until you can see a clear, sharp image (Fig. 59.b).
• To
center the sharp image
, insert the centering key into
the corresponding openings on both sides of the upper
portion of the condenser (Fig. 58.1). Move the image into
the center of the field of view (Fig. 59.c). Then, open the
field diaphragm until the image fills almost the entire im-
age field. The black edges of the image should have the
same distance from the outer edge of the field of view all
the way around. If this is not the case, center the image
again using the centering screws. Use the condenser
height adjustment to bring the edges into focus. Open the
field diaphragm until the image fills the entire field of view
and the black edges have completely disappeared from
the field of view (Fig. 59.d).
Note:
You can find additional information regarding the correct
settings for Koehler illumination in Leica Science Lab.
Link:
http://www.leica-microsystems.com/science-lab/
Fig. 58: Condenser centering
1
Centering holes
2
Height adjustment
3
Prisms and phase ring centering
4
Clamping screw
2
1
3
2
1
3
4
a
b
c
d
Fig. 59: Koehler illumination
a
Illuminated field diaphragm not focused, not centered
b
Illuminated field diaphragm focused, but not centered
c
Illuminated field diaphragm focused and centered
Diameter is too small, however
d
Field diameter (light) = Field diameter (view) (Koehler illumination)
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Содержание DMi8 A
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