6. Instrument setup– InspectIng the phase contrast rIng
Leica DMi8 with manual condenser:
• Select the light ring for your active objective on the con-
denser.
Leica DMi8 with motorized condenser:
• The correct light ring on the motorized condenser is auto-
matically swung inwards.
• If the light ring and the phase ring are not shown as ar-
ranged in Fig. 55c, the light ring must be centered and
congruent.
• Insert the centering key through the openings provided
Fig. 54.3) on both sides of the condenser.
• Turn the centering key until the dark ring (phase ring in
the objective) is congruent with the slightly narrower
bright ring (light ring in condenser) (Fig. 55c).
Caution!
When changing the objective, the centering key must no
longer be in the corresponding openings. You can lock
the condenser.
• Repeat the process for all other phase contrast objec-
tives.
• Always remove the centering keys after the centering
procedure.
Note:
You can find more information on the phase contrast meth-
od in Leica Science Lab.
Link:
http://www.leica-microsystems.com/science-lab/
Fig. 54: Condenser centering
1
Centering holes
2
Height adjustment
3
Prisms and phase ring centering
2
1
3
2
1
3
PH
LR
Fig. 55: Centering procedure for phase contrast
PH=phase contrast ring, LR=light ring
a
Condenser in bright field (BF) position
b
Condenser in phase contrast (PH) position
Light ring (LR) not centered
c
Light ring and phase ring centered
a
b
c
35
Содержание DMi8 A
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