46
9. Contrast Methods
• If necessary:
Insert the
λ
or
λ
/4 compensator into the filter
holder integrated in the condenser holder and
rotate to the left, roughly as far as the stop.
CLP/PH condenser:
Insert the
λ
or
λ
/4 compensator into the slot
on the side of the condenser.
Condensers UCLP and UCA/P:
Rotate the condenser disk into position
λ
or
λ
/4.
Alternative:
4x20 mm compensators can be pushed into
the compensator slit.
9.2 Fluorescence
• Insert a suitable specimen and rotate an
appropriate objective into place.
• Focus the image initially in transmitted light if
appropriate.
• Switch on the incident light source at the
external power unit.
• Open the shutter.
• Select an appropriate fluorescence filter
cube.
• Switch magnification changer*, if present, to
factor 1x.
• Disengage the BG 38 filter if there is no
disturbing red background. Always engage
the filter for photography, however.
• Open the aperture diaphragm.
• Open the field diaphragm until the whole field
of view is just illuminated.
If necessary center the field diaphragm.
Fig. 49
Leica DM1000 with
Fluorescence illuminator and 106z lamphousing
Содержание DM1000 LED
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