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User’s Manual Switchos II

D850R3

4-1

CHROMELEON

®

Control

4.1

Overview

This chapter provides information about how to setup the CHROMELEON

®

Software to control the Switchos II Advanced Microcolumn Switching Unit either
by the RS-232 communication port or by the event outputs of the Switchos II

loading pump. A detailed description of the software features is provided in
CHROMELEON online help (F1 key) and a quick “Getting Started” reference can
be found in the UltiMate User’s Manual. It is assumed that the user has a basic
understanding of the CHROMELEON software and its modules.

When the Switchos

™

II Advanced Microcolumn Switching Unit is used in

conjunction with the LC Packings UltiMate

™

Capillary HPLC System and the

CHROMELEON software, the flow rate of the loading pump as well as the
position of the valves of the Switchos II can be programmed from the

CHROMELEON software. The Switchos II has to be installed as described in
Chapter 2.

The role of the valves of the Switchos II is dependent on the application for

which the unit is configured (e.g. if the FAMOS Microautosampler is included in
the system). The user should ensure that the electrical interface and the fluidic
interface to other devices in the system meet the requirements of the analysis.

Note: All dialog boxes and information refer to CHROMELEON 6.5 SP3.

In this discussion, we will describe how to establish a typical column switching
application with the following parameters:

•

Sample load/clean-up time:

5 min (at a loading flow rate of 30µL/min)

•

Gradient analysis time:

50 min

CHAPTER 4

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Содержание SWITCHOS II

Страница 1: ...D850R3 www lcpackings com Switchos II Advanced Microcolumn Switching Unit User s Manual P N 162013...

Страница 2: ...al solvent hazards and the exposure to pressurized solvents October 2004 LC Packings BV A Dionex Company All rights reserved No part of this manual may be reproduced or transmitted in any form or by a...

Страница 3: ...osampler 2 3 2 3 1 Electrical Connections 2 3 2 3 2 RS 232 Connectors 2 4 2 3 3 COMMUNICATION Connector 2 4 2 3 4 INPUTS Connector 2 5 2 3 5 Triggering a Mass Spectrometer or Other Device 2 5 2 3 6 Po...

Страница 4: ...e 4 12 CHAPTER 5 Testing the Switchos II 5 1 5 1 Overview 5 1 5 2 Checking System Components 5 2 5 2 1 Solvent Bottle Caps and Degassing Unit 5 2 5 2 2 Solvent Selection Valve Basic Test 5 2 5 2 3 Sol...

Страница 5: ...ions 7 2 APPENDIX A Switching Valves A 1 A 1 Overview A 1 A 2 Maintenance A 2 A 3 Disassembly Reassembly of the Valve A 2 A 3 1 Disassembly of the Valve A 2 A 3 2 Reassembly of the Valve A 3 APPENDIX...

Страница 6: ...Table of Contents iv D850R3 User s Manual Switchos II This page intentionally left blank...

Страница 7: ...warranty period it will be repaired or replaced by LC Packings at no charge to the customer the repair replace decision is solely at the option of LC Packings All warranty requests must be received b...

Страница 8: ...negligence or otherwise for special incidental consequential or analogous damages including without limitation damages resulting from loss of use loss of profits loss of business or loss of goodwill e...

Страница 9: ...SAFETY form if applicable is attached outside of the package so that we can properly track and account for your system 2 Please include the following a Company letterhead with the following informatio...

Страница 10: ...Instructions for Returning Instruments viii D850R3 User s Manual Switchos II This page intentionally left blank...

Страница 11: ...understood before continuing Safety Precautions Note The following precautions should be followed to minimize the possibility of personal injury and or damage to property Note Make certain that you ar...

Страница 12: ...gulations Do not allow flammable and or toxic solvents to accumulate Follow a regulated approved waste disposal program Never dispose flammable and or toxic solvents through the municipal sewage syste...

Страница 13: ...wing standard EN61010 1 Safety requirements for laboratory equipment Class I Installation cat II Pollution degree II WARNING LC Packings will not accept any liability for damages direct or indirect ca...

Страница 14: ...CE Declaration xii D850R3 User s Manual Switchos II This page intentionally left blank...

Страница 15: ...present status of the switching valves microfluidic pathways the position of the solvent selection valve and the control mode A broad range of applications can be performed using micro column switchin...

Страница 16: ...D a Solvent Tray Network INPUTS RS 232 1 COMMUNICATION RS 232 2 He Line Check Valve FIGURE 1 1 Schematic Diagram of the Switchos II Advanced Microcolumn Switching Unit The Switchos II Advanced Microc...

Страница 17: ...cs controls the valve actuators and monitors the position of the three valves valve A valve B solvent selection valve Additionally it reads the COMMUNICATION port and reads out the status of the push...

Страница 18: ...in FIGURE 1 2 ON STANDBY Switch Button with LED Valve A LEDs Valve B Button with LED Valve B Backflushing Connections Pump Head Inlet Purge Screw Loading Pump Keyboard LEDs Solvent Selection Valve He...

Страница 19: ...n FIGURE 1 3 SOLVENT SELECTION Button REMOTE LOCAL Switch COMMUNICATION Connector INPUTS Connector Power Outlet to Loading Pump Main Power Switch Main Power Inlet Fuse He Inlet Power Inlet RS 232 1 Co...

Страница 20: ...n an acceptable manner Chapter 6 Maintenance and Troubleshooting describes a variety of maintenance procedures to optimize the performance of the microcolumn switching unit In addition it discusses ho...

Страница 21: ...mn Switching Unit with the LC Packings UltiMate and or CHROMELEON software please refer to the documentation provided with these products for supplemental information and to the online help of CHROMEL...

Страница 22: ...Introduction 1 8 D850R3 User s Manual Switchos II This page intentionally left blank...

Страница 23: ...r software please refer to Sections 2 4 and 2 5 for information about how to control the switching valves by relay outputs e g the Event outputs of the Switchos loading pump or the Auxiliaries of the...

Страница 24: ...ll the Switchos Advanced Microcolumn Switching Unit in areas subject to shock dust or in direct sunlight 2 2 Unpacking When the Switchos Advanced Microcolumn Switching Unit is received carefully unpac...

Страница 25: ...f the instrument FIGURE 2 1 SOLVENT SELECTION Button REMOTE LOCAL Switch COMMUNICATION Connector INPUTS Connector Power Outlet to Loading Pump Main Power Switch Main Power Inlet Fuse He Inlet Power In...

Страница 26: ...he RS 232 2 connector of the Micropump and the RS 232 1 connector of the UV Detector with the same type of cable Switchos II UltiMate PC COM port X SW II Valve Control PC COM port Y Solvent Organizer...

Страница 27: ...er signal from the CHROMELEON software at some event e g on the beginning of the gradient program connect the appropriate input of the mass spectrometer or other device to the EVENT 8 output relay con...

Страница 28: ...e loading pump using the connection cable P N 160172 presented in FIGURE 2 4 Switchos INPUTS Switchos Events EVENT3 EVENT6 EVENT7 to MS or another device GROUND GROUND EVENT8 Micropump Switchos II EVE...

Страница 29: ...f the switching valves A and B and the Solvent Selection Valve SSV are controlled by the INPUTS connector FIGURE 2 6 Contact closure relay TTL and open collector outputs can drive the Switchos inputs...

Страница 30: ...r than 1 5 seconds will switch the valve to the next solvent channel from A to B B to C and finally from D back to A Additionally the SSV can be reset to solvent channel A by applying an active signal...

Страница 31: ...ppendix C CAUTION Caution Do not use a stainless steel nut and or ferrule with the inert PAEK injection switching valves The use of stainless steel nuts or ferrules may damage the valve Use only the s...

Страница 32: ...deposit by a joint it is probable that a leak has occurred and the fitting should be cleaned and tightened When you tighten a fitting do not overtighten Check that the solvent filters are clean if not...

Страница 33: ...the loading pump using 1 16 ID silicon tubing FIGURE 2 11 Purge Valve Knob Outlet FIGURE 2 11 The Purge Valve g Open the purge valve on the loading pump by turning the purge valve knob FIGURE 2 11 ap...

Страница 34: ...nd the user can configure the unit to meet the specific needs of the laboratory In this section we show the fluidic setup of a pre concentration application using the Switchos Advanced Microcolumn Swi...

Страница 35: ...e appropriate tubing for your application Application I D Standard Version Inert Version Capillary LC 50 m P N 161480 P N 161262 1 Nano LC 20 m P N 160179 P N 161260 1 1 use PEEK fingertight fittings...

Страница 36: ...the separation After you have finished using the system flush it with a water methanol or water acetonitrile mobile phase before shutting it down Solvent should be degassed by sparging with Helium Mak...

Страница 37: ...of operation of the Switchos Advanced Microcolumn Switching Unit It includes the following information Powering Up the Switchos Section 3 2 A description of the controls on the front and rear panel Se...

Страница 38: ...atus of the Switchos e g LED 1 2 in the upper front panel indicates that valve A is in position 1 2 Note The initialization procedure takes a few seconds The instrument is ready to use when LED A of t...

Страница 39: ...Switch Button with LED Valve A LEDs Valve B Button with LED Valve B LEDs Solvent Selection Valve He Regulating Valve Valve A Valve B LEDs Valve A Microflow Outlet FIGURE 3 3 Control Elements on the U...

Страница 40: ...ower position the unit can be controlled on a local basis by the push buttons and switches on front and rear panel Any change that is made will override the present condition regardless of whether it...

Страница 41: ...ot used 9 Arrow Keys for Cursor 10 Data Input Keys FIGURE 3 5 The Front Panel of the Loading Pump 3 5 1 Setting the Flow Rate To set the flow rate use the arrow keys to move the cursor to the Flow fie...

Страница 42: ...the purge valve and then press the PURGE key on the pump item 1 FIGURE 3 5 To assure proper purging of the solvent lines set the flow rate to 0 5 mL min Select each of the four solvent bottles A B C...

Страница 43: ...ngs UltiMate Capillary HPLC System and the CHROMELEON software the flow rate of the loading pump as well as the position of the valves of the Switchos II can be programmed from the CHROMELEON software...

Страница 44: ...rt the CHROMELEON Server Configuration Select the name for the server if more than one is configured of which you want to modify the configuration Click the character in front of the server name to vi...

Страница 45: ...232 COM Port To control the unit by serial communication a Add the LC Packings Switchos II SSV device to the Timebase FIGURE 4 1 This will present the LC Packings Switchos II SSV configuration box FI...

Страница 46: ...er as well as the type are indicated on the rear panel of the Micropump e g EVENT 1 TTL EVENT 3 OC etc Make certain that the proper EVENT output is linked to the corresponding signal name of CHROMELEO...

Страница 47: ...ation box and select Virtual Channel Driver from the General tab to present the Virtual Channel Driver box FIGURE 4 5 FIGURE 4 5 The Virtual Channel Driver Box b Check the first channel and click on C...

Страница 48: ...trapcolumnpressure ULT OvenTemperature Oven Temperature FMS TrayTemperature C 1 00 Sampler Temperature Note a ULT UltiMate FMS FAMOS SW II Switchos II A typical setup is presented FIGURE 4 1 FIGURE 4...

Страница 49: ...only used UltiMate configurations are available Refer to TABLE 4 3 and choose the panel which corresponds to your instrument configuration and then load this panel from the Dionex Templates Panels Dio...

Страница 50: ...le in the toolbar to start baseline monitoring item 3 FIGURE 4 8 4 3 5 Creating a Program File Using the Wizard CHROMELEON programs are text files and modifying a program is normally done by editing i...

Страница 51: ...nm the column pressure the trap column pressure and the pump pressure CHROMELEON automatically provides a program wizard when a new program is to be created which guides through creation of the progra...

Страница 52: ...e separation column parameters e g I D length and stationary phase in the UltiMate Pump Options box FIGURE 4 11 FIGURE 4 11 The UltiMate Pump Options Box Note If you do not specify the Column paramete...

Страница 53: ...he CHROMELEON online F1 key help more details j Select the number of UV channels used and the data acquisition time in the Acquisition Options box FIGURE 4 13 FIGURE 4 13 The Acquisition Option Box Th...

Страница 54: ...pendix E of the user s manual of the UltiMate system presents 4 additional examples 4 3 7 Changing a Program File A CHROMELEON program can easily be changed by a double click on the program name in th...

Страница 55: ...operating in an acceptable manner This chapter includes Testing the Solvent Bottle Caps and Degassing Unit Section 5 2 1 Testing for the Solvent Selection Valve Section 5 2 2 Testing of the fluid pat...

Страница 56: ...ff valve on the bottle cap for solvent bottle A by closing the He regulating valve verify that the bubbles of He exit on the side frit of the sparging filter frit Check to make sure that it is possibl...

Страница 57: ...shown in FIGURE 5 2 FIGURE 5 2 Placing the Syringe on the Solvent Inlet Line e Select solvent channel A by the SSV button on the rear of the Switchos The LED corresponding to Valve A should be illumi...

Страница 58: ...e by the Remote Local switch on the back panel The LEDs of the two push buttons on the front panel should be illuminated b Toggle valve A by the corresponding push button on the front panel The LED co...

Страница 59: ...ies of activities that should be performed on a periodic basis to optimize the performance of the system and minimize down time Section 6 2 Replacing Components provides directions for replacing compo...

Страница 60: ...For further information please contact your local LC Packings office or representative TABLE 6 1 Recommended Maintenance Schedule Frequency Operation Every Day Before operating check for any air bubbl...

Страница 61: ...or piston seals For more detailed information refer to the UltiMate Micropump User s Manual 6 3 1 Replacing the Sparging Filter Frit The Sparging Filter Unit is connected via tubing which slides off t...

Страница 62: ...r unit 1 2 FIGURE 6 2 High Pressure Inline Filter f Use the socket driver P N 160073 or a wrench 11 mm to remove the filter holder item 2 FIGURE 6 2 g Remove the filter and place a new filter into the...

Страница 63: ...lf a turn at a time and remove the screws d Remove the pump head Note If the purpose of removing the pump head is to check the piston rods there is no need to disassemble the pump head any further Pul...

Страница 64: ...ay 161400 13 Check Valve Cartridge 160049 14 PEEK Adapter 162300 15 Check Valve Bushing 161071 16 Set Screw 17 Backflushing Connection 18 Connecting Tubing Pump Head 162010 2 161023 3 Stainless steel...

Страница 65: ...side should be placed as indicated in FIGURE 6 5 To re assemble the pump head a Insert the titanium inlays the two check valve cartridges item 13 FIGURE 6 5 and the two PEEK adapters item 14 FIGURE 6...

Страница 66: ...he upper and the lower bushing half a turn at a time d Tighten the two check valve bushings CAUTION Caution Make certain that the orientation mark on each check valve is placed so that the mark is on...

Страница 67: ...evices out of the housing To avoid any damage to the component make certain that it is not allowed to drop as it is being removed from the housing Note When re installing the instrument take care that...

Страница 68: ...use DANGER Danger Disconnect the instrument from the mains before inspecting changing the fuse To change the Fuse a Pull out the fuse holder item 1 FIGURE 6 7 Fuse FIGURE 6 7 The Fuse Compartment b Re...

Страница 69: ...Pmin set in SETUP menu PRESSURE LIMITS Set min value to 0 MPa to disable checking System pressure exceeded the maximum pressure Pmax set in SETUP menu PRESSURE LIMITS or in monitor screen Open the pur...

Страница 70: ...ced into position 1 2 Change the setting of the instrument controlling Switchos according to the needs of the application The Switching Valve A B does not switch Switch OFF the instrument and ON again...

Страница 71: ...re 60 80 bar 100 130 bar Trap Column Flow 30 L min 30 L min Trap Column Pressure 40 70 bar 40 70 bar Connecting Tubing I D c 50 m 20 m Response delay Time Injection to injection peak d 2 5 min 2 5 min...

Страница 72: ...y 4 pieces 160492 PEEK sleeves precision cut and polished for connections with fused silica tubing 300 m O D set of 5 pieces 160266 Set of 20 nuts and ferrules for Valco switching valves 161000 PEEK F...

Страница 73: ...9 Syringe adapter for Valco switching valves Standard Version 160177 0 5 m replacement in line filter frit 161001 Frit Holder Inert Version 161107 0 5 m replacement in line filter frit inert version 1...

Страница 74: ...tchos Standard Version 160056 Connecting Tubing Pressure Sensor to High Pressure Filter Stainless Steel 1 162010 Connecting Tubing for Pump Head Stainless Steel 2 162009 Connecting Tubing Pump Outlet...

Страница 75: ...cal control push button remote control relay open collector or TTL Position Indicator two LEDs per valve 7 1 2 Solvent Selection Valve Wetted Surfaces stator PPS polyphenylene sulfide rotor Valcon E2...

Страница 76: ...are the extreme limits the input pin can be exposed to without causing permanent damage Do NOT connect voltages lower higher than those limits 7 2 3 Communication Switching Valve A and B by RS 232 ser...

Страница 77: ...hese valves are designed to offer optimal results with the switching system as they are made from materials that are inert to materials used in liquid chromatography and are designed to provide exceed...

Страница 78: ...river to remove the socket head screws which secure the cap to the valve FIGURE A 1 Driver Rotor Stator FIGURE A 1 Exploded View of the Valco Model C2 Valve 6 port Version b To insure that the sealing...

Страница 79: ...er and do not affect the sealing force which is automatically set as the screws close the cap against the valve body c Test the valve by pressurizing the system If the valve does not hold pressure it...

Страница 80: ...The Switching Valves A 4 D850R3 User s Manual Switchos II This page intentionally left blank...

Страница 81: ...The serial number must contain six digits If the serial number of the Switchos loading pump contains only 5 digits add a leading zero e g 72835 should be entered as 072835 In Table B 1 we indicate the...

Страница 82: ...Network Identification B 2 D850R3 User s Manual Switchos II This page intentionally left blank...

Страница 83: ...in FIGURE C 1 The following steps are involved in the process a Proteins are injected and trapped onto the ion exchange cartridge b Valve A is placed in position 10 1 and non charged compounds e g det...

Страница 84: ...9 10 Jumper Column 1 Valve B presented in position 10 1 1 2 3 4 5 6 7 8 9 10 Column 3 Waste Waste Microflow from UltiMate Column 4 Microflow to UltiMate UV Detector FIGURE C 1 Fluidic Setup of the tw...

Страница 85: ...use a stainless steel nut and or ferrule with inert PAEK injection switching valves The use of stainless steel nuts or ferrules may damage the valve Use only the supplied fittings PEEK and follow the...

Страница 86: ...are inserted all the way forward into the union for a proper zero dead volume connection d Manually turn the nut into the union until it is finger tight FIGURE D 2 Attaching a PEEK Sleeve Connector t...

Страница 87: ...ng PEEK hex style nuts P N 161007 supplied with the instruments instead FIGURE D 5 FIGURE D 5 The Long PEEK Hex Style Nut To use the supplied long hex nuts a Assemble the fitting as described in Secti...

Страница 88: ...d silica tubing 300 m O D set of 5 b 161007 PEEK 1 16 Universal Fitting for Switchos INERT 10 pc long hex nut and ferrule with groove 160693 1 16 Universal Fingertight Fitting with double ferrule for...

Страница 89: ...ow Rate Setting 3 5 4 8 Fluidics Connections 2 9 2 12 Flushing the Solvent Lines 2 10 Formula 4 5 Front View 1 4 Fuse Replacing 6 10 I Initialization 3 2 Inert Version 1 6 Connecting a Nano Column D 1...

Страница 90: ...re 3 5 Signal Names 4 5 Side Panels Removing 6 8 Solvent Bottles 1 3 Solvent Bottle Caps Testing 5 2 Solvent Lines Testing 5 3 Solvent Selection Valve 1 2 3 3 Testing 5 2 Sparging Filter Frit Replacin...

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