47
Figure 33
. Example: Simple Sequence Wizard Electrokinetic Pinched Injection on a Chip.
Step B– Injection
Running the Sequence
1.
Click
Sequence
in the left pane to open the
Manual Sequence Control Panel
.
2.
Press
A
at the bottom of the control panel to run the
Loading
voltage sequence. On the Manual Sequence
Control Panel you should now see channels A-D maintaining the voltages from
3.
Table 4
. If you are viewing the microchannel on a microscope you should observe a pinched injection as in
Figure 34
.
Figure 34. Pinched Injection
4.
Press
B
to run the
Injection
step. This will sweep our injection plug onto the separation channel (ports D to B
in
Figure 34
above).
Congratulations – you have written and run your first Sequence program!