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Maintenance and care
AZURA® Detector DAD 6.1L, DAD 2.1L, MWD 2.1L Instructions, V6700
Process with syringes
Process 2
1.
Disconnect the capillaries.
2.
Connect the syringes to the liquid ports of the flow cell.
3.
Introduce each cleaning solution into the flow cell.
4.
Flush each cleaning solution back and forth between the syringes
10–12 times.
5.
Repeat the procedure until there is no noticeable improvement in
flow cell performance.
6.
Continue with the “Final Rinsing Process” below.
Final rinsing process
Process
1.
Identify the point where subsequent cleaning cycles no longer
improve the performance of the flow cell.
2.
Flush the unit with ultrapure water for a period of at least 15 minu-
tes to ensure all cleaning solutions have been completely remo-
ved and there are no persistent residues that might affect flow cell
perfor mance or stability.
The flow cell performance and stability improve noticeably.
Flush the flow cell with the solution that is going to be used in your next
application. Afterwards you can start your application
9.3.3 Cleaning protocol for KNAUER LightGuide Flow
cells
KNAUER recommends to carry out this cleaning procedure at regular
intervals (every two weeks) when using acetonitrile-containing eluents in
order to extend the life of the flow cells.
Potassium hydroxide (pellets)
Hydrogen peroxide solution (30%)
Water (MilliQ)
A syringe with Luer Lock adapter for UNF 10/32 threading
(Volume: at least 5 ml)
Waste tube
Hole plug
Production of the cleaning solution
5.7 g of potassium hydroxide are carefully dissolved while
stirring in 10 ml of water under heat generation.
The solution is added slowly with 6 ml of 30% hydrogen
peroxide solution while stirring (heat and gas evolution).
The resulting solution is finally mixed with 10 ml of water and used
directly for cleaning.
The cleaning solution should always be freshly prepared for cleaning
to ensure an optimum cleaning performance.
Result
Next step
Preliminary note
Materials
