Jenway 6300 Ser Man
27
6.0
Warning
This section gives an overview of the software functions, it is not
meant to be a detailed analysis of the software routines or code. It
must not be treated as a substitute Instruction Manual, its purpose
is to enable basic navigation through the operation and set up of the
6300 spectrophotometer sufficient to verify basic operation.
6.1 Start Up Routine
When the power to the unit is switched on a self-test routine is
activated. During these start-up tests the following parameters are
checked and must be passed before operation can continue:
Dark Level Test:
- The light beam is blocked by the dark shutter
solenoid switching in. This test checks that the output of the
detector is below a threshold level when there is no light falling on
it. The test will be failed if the sample chamber lid is left open
during the start up routine, if the dark shutter (solenoid 1) is faulty
(electrical or mechanical) or if the Detector or Detector PCB is
faulty.
Wavelength Calibration:
- This test checks for the zero order
(white) light that is reflected through the sample chamber when the
grating is in a position where it acts as a mirror. Each time the unit
is switched on this position is used as a physical reference point
against which the stored wavelength calibration data is applied.
This test is carried out in the following manner; the microprocessor
instructs the grating to drive to its minimum value of –200nm. This
should ensure that the vane attached to the grating mount breaks
the light path of the opto-coupler mounted on the monochromator
base plate that is set at a position of approximately –50nm.
If it does not receive a signal from the opto-coupler then error code
Err 7, grating position sensor not detected, is returned when the
motor stops.
In correct operation a signal is returned when the vane reaches the
opto-coupler. Then the microprocessor instructs the grating to
rotate in the reverse direction, in 1nm, steps for 200nm, or until a
signal greater than 200mV is returned from the detector (this level,