INTELLICYT iQue SCREENER, Руководство по оборудованию

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77 iQue
®
Hardware Manual: Part Number 10554 Rev C., iQue Maintenance: Clog Prevention and Management
Clog Prevention and Management
Overview
It is important to take steps to avoid clogs. This article talks about the main reasons for clogging and steps to take
for clog avoidance. It also describes how to recognize when a clog may be developing and how to take steps to
troubleshoot a clog.
Clog Prevention
iQue best practices are designed to minimize clogs. These best practices include:
• Proper system startup and shutdown.
• Proper daily, weekly and monthly cleaning and maintenance.
• Proper sample preparation using the recommended sample concentrations.
• The use of cleaning cycles between plates and within a plate as needed.
Recommended Sample Concentrations
For rapid sampling using common suspension cell lines, IntelliCyt recommends a final cell concentration of between
1-3 x10
6
cells/mL. Cell densities that are higher or lower are possible, but should be tested and optimized.
Certain cell types, particularly some kinds of adherent cells, are prone to aggregation. It may be necessary to
include small amounts of cell detachment reagent in the sample buffer to minimize this effect, or to take additional
precautions to prevent and monitor aggregation. Adequate re-suspension of the cells using the iQue shaker is one
such technique.
Cleaning Between Plates
By default, every new iQue Experiment has a post-plate clean enabled in the
Protocol of every plate:
This runs a standard 30 seconds of Decontamination solution followed by
30 seconds of Cleaning Solution and 60 seconds of water at the end of the
plate. If the samples are prone to clogging, extend the times of this post-
plate clean. IntelliCyt does NOT recommend disabling the post-plate clean.