INTELLICYT iQue SCREENER, Руководство по оборудованию

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35 iQue
®
Hardware Manual: Part Number 10554 Rev C., iQue Operations: Daily QC
Daily QC
Overview
Running daily quality control is highly recommended for the iQue Screener. A daily QC check not only ensures that
system performance is within specifications, but is also a useful diagnostic tool if issues arise. IntelliCyt makes the
QC process easy by providing reagents and pre-configured analysis templates. A daily QC check will take about 10
minutes to complete.
Required Materials
Part Number Description
90295 6 peak Validation Beads (for Red Laser and FL4 Detector)
90296 8 peak Validation Beads (for Blue Laser and FL1, FL2, FL3 Detectors)
NOTE
Daily QC procedure assumes the standard 3:1 laser configuration and default filters.
Preparing QC Samples
1. Prepare the Water Test sample
•
Add 1 mL of 0.22 μm filtered, distilled water or
equivalent to a tube.
•
It is important to use clean water for an accurate test.
2. Prepare the 8-Peak Beads sample
•
Vortex the black 8-peak validation bead bottle.
•
Dilute 4 drops of the 8-peak validation beads in 500 L of filtered, distilled water in a tube.
•
Vortex the tube.
3. Prepare the 6-Peak Beads sample
•
Vortex the two white 6-peak validation bead bottles.
•
Dilute 4 drops each of the 6-peak validation beads (for 8 drops total) in 500 μL of filtered, distilled water in a tube.
•
Vortex the tube.
These diluted working solutions can be stored at 4˚C and protected from light for re-use. Assign a one-week
expiration from date of preparation. Solutions can also be prepared fresh each day, and discarded after use.
NOTE
Be sure to vortex the bottles of validation solutions before use and to use clean
tubes and water to ensure a proper bead preparation.