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Gas Incubation System

5

Operation

21

5.1

Installation and Connection

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21

5.1.1

Connection to the Gas Supply

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22

5.1.2

Connection of the Heated Tubing Package and the Humidifying Column

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23

5.1.3

Connection of Humidity Sensor Cable

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24

5.1.4

Connection of Power Supply (and USB optionally)

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25

5.2

(Re)-Filling the Humidifying Column

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25

5.3

Switching on the System

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26

5.4

Setting Parameters in the Front Display

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27

5.4.1

Run/Stop Mode

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27

5.4.2

Incubation Parameters

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28

5.4.3

Setup Menu

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29

6

IncuControl Software

31

7

Maintenance

32

7.1

CO

2

Sensor Auto-Recalibration

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32

7.2

Disinfection and Cleaning

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32

7.2.1

Wiping the Outer Surface

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32

7.2.2

Cleaning the Column’s Inner Face

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32

7.3

Influence of Surrounding Temperature and Airflow

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32

8

Troubleshooting

33

8.1

Focus not Stable

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33

8.2

Evaporation Is too High

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33

8.3

Gas Flow too Low

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33

8.3.1

Gas Input Open Without Connected Gas

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33

8.3.2

Gas Pressure too Low

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34

8.3.3

Set Values Are too High or too Low

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34

8.4

Condensation Inside the Stage Top Incubator

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34

Instruction Manual

5

Version 2.2 (May 15, 2019)

Содержание 11920

Страница 1: ...idi Gas Incubation System for CO2 Version 2 2 Instruction Manual ibidi Gas Incubation System 11922 ibidi Gas Incubation System for CO2 and O2 www ibidi com Flow I 9 9 S 10 0 Run CO2 Hum 5 1 5 0 75 2 80 0 Setup ...

Страница 2: ...Gas Incubation System Version 2 2 May 15 2019 2 Instruction Manual ...

Страница 3: ...bation System Contact ibidi GmbH Lochhamer Schlag 11 82166 Gra felfing Germany Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E mail info ibidi de Internet ibidi com Instruction Manual 3 Version 2 2 May 15 2019 ...

Страница 4: ...ting the Gas Incubation System 12 1 10 Repairing the Gas Incubation System 13 1 11 Waste Disposal WEEE RoHS Compliance Statement 13 1 11 1 EU Directive WEEE 13 1 11 2 EU Directive RoHS 13 2 Intended Use 14 3 Principle 14 4 Equipment 15 4 1 Components of the Gas Incubation System 15 4 2 Gas Mixer CO2 or CO2 O2 17 4 3 Humidifying Column 18 4 4 Heated Tubing Package 18 4 5 Humidity Sensor 19 Version ...

Страница 5: ... Mode 27 5 4 2 Incubation Parameters 28 5 4 3 Setup Menu 29 6 IncuControl Software 31 7 Maintenance 32 7 1 CO2 Sensor Auto Recalibration 32 7 2 Disinfection and Cleaning 32 7 2 1 Wiping the Outer Surface 32 7 2 2 Cleaning the Column s Inner Face 32 7 3 Influence of Surrounding Temperature and Airflow 32 8 Troubleshooting 33 8 1 Focus not Stable 33 8 2 Evaporation Is too High 33 8 3 Gas Flow too Lo...

Страница 6: ...meanings in this man ual Do not proceed beyond a signal word until you have performed the indicated actions WARNING A potentially hazardous situation which if not avoided could result in se rious injury or even death Warning messages in the text are displayed in a gray shaded box CAUTION A potentially hazardous situation which if not avoided could result in minor or moderate injury It is also used...

Страница 7: ...e to Humidifying Column DC 24 V max 3 6 A Operating Conditions Operating site Indoor use only Operating temperature 18 30 C 64 86 F Operating humidity max 80 relative humidity RH Operating altitude max 2000 m atmospheric pressure 800 1060 hPa 11 6 15 4 psi Storage Conditions 5 50 C 23 122 F humidity 60 relative humidity RH Outer Dimensions and Characteristics of the Components Gas Mixer 90 170 230...

Страница 8: ... 5 psi optimum 0 8 1 2 bar 11 6 17 4 psi Nitrogen N2 Purity 99 N2 1 bar 14 5 psi optimum 0 8 1 2 bar 11 6 17 4 psi only for the CO2 O2 version CO2 Control Control range 0 15 Accuracy 0 2 vol 3 of reading e g 0 35 at 5 CO2 concen tration Resolution 0 1 Self calibration At least once per week the Gas Mixer must be either turned off for five minutes or the CO2 concentration must be set to 0 for five ...

Страница 9: ...n Humidity Control Humidity sensor type Sensirion SHT31 Control range 20 99 lower limit dependent on environment and gas supply min set humidity is 0 Sensor accuracy 2 5 max Resolution 0 1 Humidifying Column Temperature Control Control range Ambient water temperature to 55 C min set temp 0 C Accuracy 2 C Resolution 0 1 only controllable on the Gas Mixer not in IncuControl Flow Control Mixed Gas Co...

Страница 10: ...es by power cables with inadequate specifications By violating these instructions you risk electric shock and fire Only use extension cables that have a protective ground wire Do not operate the Gas Incubation System under conditions that pose a risk of explosion implo sion or the release of gases Only operate the Gas Incubation System with aqueous solutions Do not operate a damaged Gas Incubation...

Страница 11: ... a table bench or desk upon which the instrument is installed Install the Gas Incubation System in a location that enables easy access for maintenance Do not place heavy objects on the instrument The Gas Mixer needs a gas input of 1 bar 14 5 psi Only operate the Gas Mixer with the pressure indicated in the specifications on page 7 1 7 Regulatory Statement The Gas Incubation System has been designe...

Страница 12: ...g gases 7 Accidents beyond ibidi s control including natural disasters This warranty does not cover consumables such as cell culture chambers and dishes tubes fluidic connectors reagents etc The warranty for all parts supplied and repairs provided under this warranty expires on the warranty expiration date of the original product 1 9 Transporting the Gas Incubation System The weight of the Gas Mix...

Страница 13: ...nion s Waste Electrical and Elec tronic Equipment WEEE Directive The symbol indicates that this product must be recycled dis posed of separately from other household waste It is the end user s responsibility to dispose of this product by taking it to a designated WEEE collection facility for the proper collection and recycling of the waste equipment The separate collection and recycling of waste e...

Страница 14: ...d with the Heating System providing a fully con trolled stage top chamber 3 Principle Physiological Conditions in Live Cell Imaging Whereas the Heating System1 controls the temperature of the incubation chamber the Gas Incuba tion System provides a controlled atmosphere of gas CO2 and optionally O2 as well as a defined humidity in the emitted gas The humidified atmosphere prevents the samples with...

Страница 15: ...ption Components 11920 Gas Incubation System for CO2 Gas Mixer CO2 Humidifying Column Heated Tubing Package Gas Tubing for CO2 and Air Humidity sensor Humidity sensor cable USB cable Power cable USB flash drive Wrench for humidity sensor 11922 Gas Incubation System for CO2 and O2 Gas Mixer CO2 O2 Additional to all parts of 11920 Gas Tubing for N2 Dead end tubing Table 3 Overview of the components ...

Страница 16: ...e the N2 gas inlet Humidity sensor with counter nut to fix on the Heated Lid Humidity sensor cable Cable to connect the Gas Mixer to the hu midity sensor USB cable to connect the Gas Mixer with a computer External power supply for the Gas Mixer Country specific power cord to connect the external power supply to the wall socket USB flash drive with IncuControl software Wrench to fix the humidity se...

Страница 17: ... dry Figure 2 Front view of the Gas Mixer The LEDs on the front indicate the status of the channels connection to the power supply connection to the USB gas control and humidity control Figure 2 Table 4 Control LEDs of the Gas Mixer LED Mode Significance LED off Channel inactive LED on Channel active LED fast blinking Channel error On the back of the Gas Mixer all connections for cables and tubing...

Страница 18: ...as two gas connections input and output as well as an electric plug for the heating of the column connection see Section 5 1 2 On top of the column is an opening for filling it with water filling see Section 5 2 The maximum 130 ml and minimum 50 ml levels are indicated by the red labels inside the column see Figure 4 Figure 4 Humidifying Column 4 4 Heated Tubing Package The Heated Tubing Package c...

Страница 19: ...G merging point where the heating wire is led into the tubing H gas tubing to the Heated Chamber I Caution Do not bend or cut the Heated Tubing The heating wire inside the tubing may be damaged and become non functional 4 5 Humidity Sensor The humidity sensor is fixed in the side wall of the Heated Lid of the Heating System and measures the humidity inside the incubation chamber It sits inside a s...

Страница 20: ... against the outer wall of the Heated Lid using the wrench provided with the Gas Mixer The flat cut away on the counter nut should be facing away from the lid allowing the wrench to fit properly over the nut Be careful not to over tighten the nut Counter nut Through hole Polymer block with humidity sensor Spacer nut Figure 7 Installation of the humidity sensor in the side wall of the Heated Lid Ca...

Страница 21: ...tion The Gas Mixer and Humidifying Column should be placed next to the microscope stand The tubing of the Heated Tubing Package must reach the Heated Lid and Heated Plate on the microscope stage Caution Do not place the Gas Mixer and Humidifying Column on the microscope stage The vibration might disturb the measurement All tubing and cables are connected to the back of the Gas Mixer Figure 8 Figur...

Страница 22: ...9 Connection of the gas tubing to the Gas Mixer The N2 port is closed and not available in the CO2 version To connect the gas tubing to the Gas Mixer push the tubing into the push in fittings The functional principle is shown in Figure 10 Push A Push Push B Pull Figure 10 How to connect and disconnect the gas tubing to the push in fittings Push in to connect A Push and pull to disconnect B Caution...

Страница 23: ...de Gas Mixer output 1 white Blue tubing with white label Gas Mixer output 2 yellow Blue tubing with yellow label Humidifying Column output red Blue tubing with red label Humidifying Column output blue Blue tubing with blue label 1 Connect the two gas outlets of the Gas Mixer to the white 1 and yellow 2 marked gas tubing to Output 1 and Output 2 on the back wall of the Gas Mixer 2 Connect the plug ...

Страница 24: ...ng Column as this can also damage the gas mixer 5 1 3 Connection of Humidity Sensor Cable The humidity sensor should be mounted in the Heated Lid of the Heating System see Section 4 5 To operate the Gas Incubation System with humidity control the humidity sensor cable must be connected between the Gas Mixer and the humidity sensor 1 Plug the humidity sensor cable into the Gas Mixer in the connecto...

Страница 25: ...rs of the experiment 1 Plug the power supply into the connector on the Gas Mixer labeled Power 2 Connect the other end to the wall socket 3 Optional Connect the computer to the Gas Mixer with the supplied USB cable 5 2 Re Filling the Humidifying Column Before the first use the Humidifying Column must be filled with 130 ml of ultra pure water During operation the column should always be refilled be...

Страница 26: ...cause serious burns 5 3 Switching on the System After connecting all cables and tubing and after filling the Humidifying Column ultra pure water the system is ready to start 1 Set your gas regulation valve to the recommended pressure of 1 bar 14 5 psi 2 Switch on the Gas Mixer and wait for self test all systems and heat up only CO2 O2 versions 3 Set the parameters on the display Section 5 4 4 Chec...

Страница 27: ...igure 15 Gas Mixer front display and set buttons The display shows the measured I instantaneous and set S set temperatures of all the chan nels The cursor position is indicated with square brackets You can move the cursor using the left and right buttons If you want to select a parameter or a function press the round button and the square brackets will change to angle brackets Now you are able to ...

Страница 28: ... below 7 l h gas mixes may not reach the highest possible humidities If this is the case augment the gas flow rate until the humidity is reached O2 regulation hypoxia Gas Mixer for CO2 and O2 only To generate hypoxia conditions set the oxygen to the target value The O2 will be replaced by nitrogen N2 until the set value is reached Please keep in mind that in a CO2 air mixture the O2 concentration ...

Страница 29: ...elect the respective channel and confirm with the round button 3 Select On or Off and confirm with the round button 4 To move on navigate to Return and press the round button Table 8 Channel numbers are dedicated as follows Channel number Parameter Channel 1 Flow outlet gas flow rate Channel 2 CO2 Channel 3 Humidity Channel 4 T C Humidifying Column Temperature Channel 5 O2 CO2 O2 version only Alar...

Страница 30: ...ences Set the brightness and contrast of the display in the preferences menu Preferences Setup Backlight 65 Contrast 15 Return Figure 20 Preferences dialog Info Info about serial number and firmware version is shown in the info dialog Figure 21 Info display Version 2 2 May 15 2019 30 Instruction Manual ...

Страница 31: ...d from the ibidi website For more details please refer to the IncuControl instructions Figure 22 Gas Mixer control window in IncuControl Prohibitions on the use of ibidi software Copying software for other than backup purposes Transfering or licensing of the right to use software to a third party Disclosure of confidential information regarding software Modification of software Instruction Manual ...

Страница 32: ...hanol or isopropanol for an extended period of time It can lead to damage of the Humidifying Column 7 2 2 Cleaning the Column s Inner Face Microbial contamination in the Humidifying Column is usually not a problem since distilled water and light starved environments microscopy rooms do not support microbe growth If however contamination has occurred disinfect the Humidifying Column by running the ...

Страница 33: ...y Additionally we suggest using silicone oil e g Anti Evaporation Oil ibidi 50051 to decrease evaporation Covering the medium with sterile silicone oil prevents all evaporation effects and is compatible with cell culture Please do not use mineral oil as this can be harmful to your cultureware Equilibrate oil and medium inside the incubator overnight This step helps to avoid the formation of air bu...

Страница 34: ...that the maximum O2 concentration depends on the set CO2 concentration See page 28 for further details 8 4 Condensation Inside the Stage Top Incubator Check the temperature of the chamber Heated Lid and Heated Plate Make sure the humidity sensor is not in contact with the Heated Lid In case of condensation decrease the humidity and air dry the incubator if necessary Please contact ibidi at techsup...

Страница 35: ...Gas Incubation System Instruction Manual 35 Version 2 2 May 15 2019 ...

Страница 36: ...bH Lochhamer Schlag 11 82166 Gräfelfing Germany Toll free within Germany Phone 0800 00 11 11 28 Fax 0800 00 11 11 29 International calls Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E Mail info ibidi com www ibidi com ...

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