Gas Incubation System
2
Intended Use
The Gas Incubation System provides defined gas control for live cell imaging experiments. It is
intended to be used with stage top incubators and can be applied on various microscopes. The Gas
Incubation System mixes the required gas ratios in the output and actively humidifies this mixed gas
output before the gas enters the incubation chamber.
Two versions of the Gas Incubation System are available: the ibidi Gas Incubation System for CO
2
and the ibidi Gas Incubation System for CO
2
and O
2
. Both versions can control the CO
2
concentration
and humidity. The O
2
version can additionally downregulate the amount of O
2
in the gas mixture to
create hypoxia.
The ibidi Gas Incubation System can be combined with the Heating System, providing a fully con-
trolled stage top chamber.
3
Principle
Physiological Conditions in Live Cell Imaging
Whereas the Heating System
controls the temperature of the incubation chamber, the Gas Incuba-
tion System provides a controlled atmosphere of gas (CO
2
and optionally O
2
) as well as a defined
humidity in the emitted gas. The humidified atmosphere prevents the samples with living cells from
evaporation of the culture medium.
The gas stream is flushed continuously through the stage top incubator and evades through the gaps
of the incubation chamber.
Heated Plate
Heated Lid
40°C
37°
Gas mixture,
Humidity
Humidity Sensor
Humidity
O
2
Levels
CO
2
Levels
Temperature
Cell culture vessel
Figure 1 – Schematic cross-sectional view of the Gas Incubation System combined with the Heating
System.
1
Details about the Heating System are given in the Heating System instructions.
Version 2.2 (May 15, 2019)
14
Instruction Manual
