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Table 3-2 Characteristics of Organic Solvents

Polarity
E

(Al

)

Viscosity
(cP20



Refractive
Index

UV Cut
off (nm)

Flash
Point (



Ignition Point

Vapor
Density
(Air: 1)

Boiling
Point (



Specific
Gravity
(Water: 1)



Lower
Limit

Upper
Limit

Fluoroalkanes

-0.25

1.25

n-Pentane

0.00

0.23

1.358

210

< -40

308.9

1.5

7.8

2.5

36.1

0.6

Hexane

0.00

1.375

210

-21.7

233.9

1.2

7.5

3.0

68.9

0.7

Isooctane

0.01

1.404

210

Petroleum ether

0.01

0.3

210

n-Decane

0.04

0.92

1.412

46.1

207.8

0.8

5.4

4.9

173.9

0.7

Cyclohexane

0.04

1.00

1.427

210

-20

260

1.3

2.9

81.7

0.8

Cyclopentane

0.05

0.47

1.406

210

Diisobutylene

0.06

1.411

210

i-Pentene

0.08

1.371

-17.8

272.8

1.5

8.7

2.4

0.7

Carbon disulfide

0.15

0.37

1.626

380

-30

100

1.3

2.6

46.1

1.3

Carbon tetrachloride

0.18

0.97

1.466

265

Amyl chloride

0.26

0.43

1.413

225

12.8

343.3

1.6

8.6

3.7

106.1

0.9

Butyl chloride

0.26

1.436

220

-9.4

460

1.8

10.1

3.2

0.9

0.81

†

o-17.2

463.9

1.0

6.0

144.4

Xylene

0.26

0.61

†

~ 1.50

290

m-25

527.8

1.1

7.0

3.7

138.9

0.9

0.64

†

p-25

528.9

1.1

7.0

138.3

i-Propyl ether

0.28

1.368

220

-27.8

443.3

1.4

3.5

68.9

0.7

i-Propyl chloride

0.29

0.37

1.378

225

-32.2

593.3

2.8

10.7

2.7

0.9

Toluene

0.29

0.33

1.496

285

4.4

536.1

1.4

6.7

3.1

110.6

0.9

n-Propyl chloride

0.30

0.59

1.389

225

< -17.8

2.6

11.1

2.7

46.1

0.9

Chlorobenzene

0.30

0.35

1.525

32.2

637.8

1.3

7.1

3.9

132.2

1.1

Benzene

0.32

0.80

1.501

280

-11.1

562.2

1.4

7.1

2.8

0.9

Ethyl bromide

0.37

0.65

1.424

511.1

6.7

11.3

3.8

37.8

1.4

Ethyl ether

0.38

1.353

220

-45

180

1.9

2.6

0.7

Ethyl sulfide

0.38

0.23

1.442

290

Chloroform

0.40

0.45

1.443

245

Methylene chloride

0.42

0.57

1.424

245

-50

518.9

3.8

15.4

2.2

38.5

0.9

Methyl i-butyl ketone

0.43

0.44

1.394

330

Tetrahydrofurane

0.45

1.408

220

-14.4

321.1

11.8

2.5

66.1

0.9

Ethylene dichloride

0.49

1.445

230

13.3

412.3

6.2

3.4

83.9

1.3

Methyl ethyl ketone

0.51

0.79

1.381

330

-6.1

515.6

1.8

2.5

0.8

i-Nitropropane

0.53

1.400

380

48.9

420.6

2.6

3.1

131.1

1.0

Acetone

0.56

1.359

220

-17.8

537.8

2.6

12.8

2.0

56.7

0.8

Dioxane

0.56

0.32

1.422

260

12.2

180

2.0

3.0

101.1

1.0

Ethyl acetate

0.58

1.54

1.370

260

4.4

460

1.8

3.5

0.9

Methyl acetate

0.60

0.45

1.362

210

-10

501.7

3.1

2.6

0.9

Amyl alcohol

0.61

0.37

1.410

32.8

300

1.2

10.0

3.0

137.8

0.8

Dimethyl sulfoxide

0.62

4.1

Aniline

0.62

2.24

1.586

617.2

1.3

3.2

184.4

1.0

Dimethyl amine

0.63

4.4

1.387

275

< -17.8

312.2

1.8

10.1

2.5

56.7

0.7

Nitromethane

0.64

0.38

1.394

380

418.3

7.3

2.1

101.1

1.1

Acetonitrile

0.65

0.67

1.344

210

5.6

1.4

81.7

0.8

Pyridine

0.71

0.37

1.510

305

1.8

12.4

2.7

115

1.0

Butyl cellosolve

0.74

0.94

220

i-Propanol n-Propanol

0.82

1.38

210

11.7

398.9

2.0

2.1

82.8

0.8

Ethanol

0.88

2.3

1.361

210

12.8

422.8

4.3

1.6

78.3

0.8

Methanol

0.95

1.20

1.329

210

11.1

463.9

7.3

1.1

63.9

0.8

Ethylene glycol

1.11

0.60

1.427

210

111.1

412.8

3.2

197.2

1.1

Acetic acid

Large

19.9

1.372

Water

Large

1.26

1.333

Salts & buffer

Larger

* L.R.Snyd

ar, Dekker. “Principles of Adsorption Chromatography”

†

The Handbook of Chemistry, 2nd ed., The Chemical Society of Japan

Solvent with low boiling point as shown in cannot be used.

Содержание PRIMAIDE 1440

Страница 1: ...PRIMAIDE 1440 荧光检出器 ...

Страница 2: ...China Copyright C Hitachi Instruments Dalian Co Ltd 2021 5th Edition 2021 All rights reserved Printed in China Part No 8DD 9705 Before using the instrument read the safety instructions and precautions carefully Be sure to observe the safety instructions in the manual and the WARNING CAUTION labels on the instrument Keep this manual in a safe place nearby so it can be referred to whenever needed ...

Страница 3: ...tted or disclosed to a third party in any form or by any means without the express written permission of Hitachi Instruments Dalian Co Ltd 3 Hitachi Instruments Dalian Co Ltd assumes no liability for any direct indirect or consequential damages arising from use not described in this manual 4 This document does not provide any warranty or permission for industrial properties or any rights to grant ...

Страница 4: ...ences adverse to safety Note that it is allowed only for persons having a basic knowledge of chemical analysis procedures to use this instrument Carefully read this instruction manual before attempting operation For proper use of the software please acquaint yourself with it The liquid chromatograph system comprises a pump unit for delivering mobile phases a sample introduction unit for injecting ...

Страница 5: ...Section 1 OUTLINE Basic Operation Section 2 FUNCTIONS Name and Function of Each Part on FL detector Section 3 OPERATION Basic Operation Section 4 MAINTENANCE TROUBLESHOOTING Operational Check Section 5 SPARE PARTS Replacement Parts and Consumables Section 6 INDEX APPENDIX1 INSTRATTION APPENDIX2 DESCRIPTION ON CONTACT SIGNAL COMMUNICATION APPENDIX3 GLOSSARY First of all read IMPORTANT and SAFETY SU...

Страница 6: ... of Warranty Any parts which prove to be defective in design or workmanship during the warranty period will be repaired adjusted or replaced without charge A substitute part may be used for repair or replacement with an equivalent product may be made instead of repair Such system components as a personal computer and printer to be updated frequently for improvement may not be available in original...

Страница 7: ...rements specified by the manufacturer b Failure due to power supply voltage frequency other than specified by the manufacturer or due to abnormality in power supply c Corrosion or deterioration of the tubing due to impurities contained in reagent gas air or cooling water supplied by the user d Corrosion of the electric circuits or deterioration of the optical elements due to highly corrosive atmos...

Страница 8: ...uld foresee or has been advised of the possibility of such damages Disclaimer of Liability for Industrial Properties of a Third Party Hitachi Instruments Dalian Co Ltd does not assume any liability for a third party s complaint regarding infringement of any patent rights or industrial properties with respect to products manufactured through use of the equipment supplied by Hitachi Instruments Dali...

Страница 9: ... of the instrument other than maintenance parts and consumables may not be available in the event of discontinuance of manufacture of the main unit If repair cannot be provided in the case of instrument malfunction we recommend that you consider discontinuing use and replacing the instrument Technical Seminars and Training Courses for Users We offer technical seminars and training courses at eithe...

Страница 10: ...chargeable battery mounted on the PC refer waste disposal treatment servicing to an authorized waste collection agent or send the battery for recycling after providing insulation on its positive and negative terminals with adhesive vinyl tape or the like Precautions on CE Conformity Marking In consideration of use in the European countries this instrument bears the CE mark indicating the conformit...

Страница 11: ...product indicates the requirement NOT to dispose of the equipment as unsorted municipal waste but use the return and collection systems available RoHS Requirement This instrument also conforms to the RoHS Directive 2011 65 EU and has the CE conformity marking for it The instrument is designed to RoHS the European Norm EN IEC63000 2018 Information on Disposal for Users In the European Union If you ...

Страница 12: ... to this instrument because of possible infection with bacteria or viruses Disturbance by Electromagnetic Wave This instrument conforms to Class A in the EN standards EN61326 2013 Avoid installing this instrument near equipment whose data will be affected by electromagnetic noise within the permissible range of this standard Also data of this instrument may be affected by electromagnetic noise or ...

Страница 13: ...related to personal injury hazards but concern the proper use of the product The following signal word NOTICE or NOTE is used to indicate precautionary instructions concerning possible property damage NOTICE Used to indicate a potentially hazardous situation which if not avoided will or can result in damage to user s property serious damage to the instrument damage to data or environmental polluti...

Страница 14: ...tric shock or fire Radiation from the Laser Light Source WARNING The internal CD ROM or CD R W drive of the PC is provided with a laser beam source In normal operation of the PC containing the laser beam source no laser beam harmful to human health will be emitted outside Note however that if a laser beam leaking out of the PC accidentally gets into the eye eye injury could occur When using the PC...

Страница 15: ...age of solvent or waste solution Leakage of solvent inside the instrument When using flammable chemicals be careful about possible ignition due to static electricity To prevent the build up of static electricity use a conductive container for waste solution employ and provide proper grounding connection to it Explosion of Vapor from Flammable Chemicals If a flammable chemical such as organic solve...

Страница 16: ...ble may result in an electric shock hazard Connect the 3P power cable to a grounded 3P power outlet b If a grounded 3P power outlet is not available use a grounded 3P table tap or a 3P 2P adapter In this case be sure to provide proper grounding connection For grounding connection use a screw having a diameter of M4 or higher and a turn count of 3 or more in threading and a wire having a thickness ...

Страница 17: ...rs 890 2019 Long life xenon lamp 1 000 hours At replacement Before removing the xenon lamp for replacement turn off the xenon lamp turn off power to the instrument and then wait for at least one hour until the xenon lamp becomes sufficiently cool to reduce its internal pressure the normal safety level If a strong shock or impact is applied to the xenon lamp or if the surface of its quartz glass pa...

Страница 18: ...de consumed excessively is used continuously pressure inside the lamp bulb may become too high causing possible explosion To prevent this replace the lamp with a new one immediately if its cathode has been consumed substantially If the nut on the lamp base and wiring part is loose the contact resistance between them will increase due to poor contacting This could generate a large amount of heat to...

Страница 19: ...irectly when it is lit Be sure to wear tinted safety glasses to prevent possible eye damage Heavy Instrument This instrument weight as much as 26 7 kg When carrying this instrument exercise care not to incur injury by dropping it accidentally Be sure to hold the right and left parts of the instrument securely when moving it Prevention of Instrument Tipover Personal injury could be incurred if the ...

Страница 20: ...ual parts Periodically check the appearances of these warning labels to see if they are clean to allow easy reading over a safe distance If any one of the warning labels becomes illegible due to deterioration contact your local Hitachi High Tech Science Corporation sales representative or service office of Hitachi High Tech Science Corporation sales representative for replacement with a new one Re...

Страница 21: ...strument Front View 図 2 Explosion of Vapor from Flammable Chemicals 3 Electric Shock due to Contact with Light Source Power Supply 6 Explosion of Xenon Lamp 7 Burns due to Contact with Hot Part 5 Ignition of Flammable Chemicals 4 Before Turning on Xenon Lamp ...

Страница 22: ...s too small Furthermore leakage of a highly volatile solvent may not be detected Be sure to check for liquid leakage regularly to prevent a possible serious accident regardless of whether the leak sensor is activated or not c In routine inspection of the instrument visually check for liquid leakage before attempting measurements d In periodic inspection of the instrument check that the leak sensor...

Страница 23: ... of occurrence of a carryover Otherwise a correct judgment may not be formed Since fluororesin and quartz materials are used for the wetted parts of piping particular attention should be paid when analyzing samples that are likely to be adsorbed by these materials Before Turning on Xenon Lamp Again After the xenon lamp is turned off it remains very hot for a while Under this condition it is not al...

Страница 24: ...Y 1 General Safety Precautions SAFETY 2 WARNING Precautions in the Manual SAFETY 3 CAUTION Precautions in the Manual SAFETY 7 WARNING and CAUTION Labels on Instrument SAFETY 8 NOTICE Precautions SAFETY 11 1 OUTLINE 1 1 1 1 Isocratic System 1 1 1 2 Low Pressure Gradient System 1 2 2 FUNCTIONS 2 1 2 1 Name and Function of Each Part on FL detector 2 1 2 2 Name and Function of Each Part on UI Pad Opti...

Страница 25: ...am stopping 3 33 3 4 4 Time program operation examples 3 33 3 4 5 Spectrum measurement 3 37 3 4 6 Recorder and spectrum output factors setting 3 41 3 4 7 Marker operation 3 45 3 5 Set Up Setting 3 46 3 5 1 Communication channel CH setting 3 46 3 5 2 Liquid crystal display contrast setting 3 47 3 5 3 Standard xenon lamp lifetime setting 3 49 3 6 Before Performing Analysis 3 51 3 6 1 Selection of mo...

Страница 26: ...nt Parts and Consumables 5 1 5 2 Lamp Replacement 5 3 5 2 1 Xe lamp replacement 5 3 5 2 2 Hg lamp replacement 5 17 5 3 Fuse Replacement 5 20 6 INDEX 6 1 APPENDIX APPENDIX 1 INSTALLATION APPENDIX1 1 1 Unpacking APPENDIX1 1 2 Installation Conditions APPENDIX1 2 3 Installation Place APPENDIX1 5 4 Installation Environment APPENDIX1 5 5 Checking the Contents APPENDIX1 6 6 Assembling APPENDIX1 7 6 1 Rem...

Страница 27: ...nd other optional devices are incorporated in the system 1 1 Isocratic System The isocratic system has a simple configuration in which single solvent analysis is performed Fig 1 1 Isocratic System 1 An eluent is fed by the pump through the degassing device 2 A sample is injected from the autosampler 3 The injected sample is separated through the column which is maintained at a constant temperature...

Страница 28: ... pressure unit the eluents are mixed at a pre specified ratio 3 Through the mixer the mixture eluent is delivered 4 A sample is injected by the autosampler 5 The injected sample is separated through the column which is maintained at a constant temperature by the column oven 6 Each component separated from the sample is detected as a signal by the detector Primaide 1440 Fluorescence Detector Primai...

Страница 29: ...ed for indicating the following states Drain tray A tray for liquid leakage Designation Color LED Indication CH1 blue orange Blue CH1 operation mode Orange An error has occurred in CH1 operation mode CH2 blue orange Blue CH2 operation mode Orange An error has occurred in CH2 operation mode Xe LAMP blue Blue Xe lamp is lit PROG RUN blue Blue Time program is in execution LED Power switch UI pad Drai...

Страница 30: ...eive the instrument parameter data via the e line 1 The florescence intensity data is output 2 Used for input output of each contact signal 2 START IN The program is initiated ERROR IN An error condition in another instrument is checked ERROR OUT An error condition on this instrument is indicated BUSY OUT The operational status of the instrument is indicated 2 LAMP OFF IN The lamp is turned off by...

Страница 31: ...og output for data processor integrator 1 The florescence intensity value at each point of time 2 Stored spectral data The florescence intensity value data is output 2 2 Name and Function of Each Part on UI Pad Option A UI pad will be used when a chromato data station won t be used for an instrument controlling The UI pad will be invalid when the chromato data station is connected Description is g...

Страница 32: ...elength accuracy Displays and resets lamp logbook 2 Sets the key lock 2 Press the ESC key to release the key lock 5 PROG INSERT Set program Selects and sets the time program 6 Arrow 1 The monitor screen key Accesses the previous screen key Accesses the next screen 2 Editing program key Displays the previous screen Indicates the last line if it is the first line key Displays the next screen Indicat...

Страница 33: ...ointed while editing a time program Deletes the step which the cursor is pointed in the input part of TIME Deletes the item when the cursor points a part other than the input part of TIME When the ESC key is pressed while is displayed on the screen the screen returns to the monitor screen and cancels the DEL key processing is displayed after pressing the DEL key and the processing will be determin...

Страница 34: ...olecule for the various processes involved in absorption fluorescence and phosphorescence Radiationless transition Radiationless transition Absorption Excitation light Excited triplet state Excited state V Phosphorescence Fluorescence Ground state V 3 0 1 2 3 0 1 2 Light Light Excitation Stable condition Ground state Fluorescence or Phosphorescence Unstable condition Excited state ...

Страница 35: ...absorbed by the substance is lost as vibrational energy the energy emitted by the excited state is less than that absorbed by the compound i e the fluorescence wavelength is longer than the excitation wavelength Stokes Law The ratio of the number of photons emitted during fluorescence to the number of photons absorbed is called the quantum efficiency of fluorescence Fluorescence Yield If two compo...

Страница 36: ...he uncertainty in the concentration measurement is 20 In contrast in fluorometry a difference in the signal is directly proportional to the concentration of sample An analysis of the error in the measurement is as follows Output signal level at measurement of sample Value corresponding to blank 100 0 1 0 0 1 Difference proportional to concentration of sample 100 0 2 As is evident from this example...

Страница 37: ... wavelength is different from the excitation wavelength incident beam wavelength scattering due to the excitation radiation is negligible Fig 2 5 Comparison of Absorptiometry and Fluorometry In addition to providing high sensitivity fluorescence detection can provide a fluorescence spectrum and an excitation spectrum which is very similar to the absorbance spectrum If the sample contains two compo...

Страница 38: ...f a Sample Containing Two Components Table 3 1 compares the analytical information obtained via absorptiometry and fluorometry Table 2 4 Comparison of Analytical Information Attainable with Absorptiometry and Fluorometry Absorptiometry Fluorometry Absorption spectra only corresponding to excitation spectra Excitation spectra Fluorescence spectra Absorption spectra Component A Component B Excitatio...

Страница 39: ...changes Both Rayleigh and Raman scatterings occur due to a solvent Take care not to mistake them for fluorescence peaks Table 3 2 presents the position of the Raman peak for a variety of excitation wavelengths In liquid chromatography the Raman scattering appears as a background rise This means that baseline noise increases to disturb high sensitivity measurement Table 2 5 Raman Spectral Peak Posi...

Страница 40: ...e excitation beam is substantially absorbed near the entrance slit of a cell and it cannot reach the cell center adequately An extreme situation is shown in Fig 3 8 Fluorescence is emitted near the entrance slit of a cell but it will not be taken into the emission monochromator Fig 2 8 Sample Having Extremely High Concentration Raman scattering 80 0 20 40 60 350 400 Relative intensity Excitation w...

Страница 41: ...orescence occurs when the tail of the shorter wavelength side of fluorescence spectrum overlaps the tail of the long wavelength side of the excitation spectrum When this phenomenon occurs the fluorescence spectrum appears to be somewhat shifted toward the longer wavelength This phenomenon will very rarely cause a substantial error in quantitation when fluorescence detection is used and should not ...

Страница 42: ...nd and third order radiation it is simply necessary to place a short wavelength cutoff filter in the path of fluorescing radiation When the second and or third order radiation poses a particular problem it is recommended to use the optional filter set accessory In measurement with a chromatograph the second and third order radiation appears as a background rise Therefore baseline noise will increa...

Страница 43: ...cording to the explanation given above 5 Temperature Dependency of Fluorescence Intensity In most cases the fluorescence intensity decreases by 1 to 2 as the temperature of sample increases by 1 C It should be noted however that in some biochemical samples the fluorescence intensity decreases by approx 10 as the temperature of sample increases by 1 C ...

Страница 44: ...ere it is dispersed by the monochromator The selected radiation strikes the measuring detector On the other hand the excitation beam which passed through the sample enters the monitor detector via the diffusion plate Photomultiplier high voltage circuit Xenon lamp starter Recorder or integrator Xenon lamp ballast Xenon lamp Sample fluorescence amplifier Electrical connection Monitor detector ampli...

Страница 45: ... the instrument 2 4 Specifications 1 Optical system Ratio photometry intensity of transmitted beam is monitored 2 Light source Xe lamp 150 W Hg lamp for checking wavelength 3 Excitation wavelength range 200 to 850 nm Zero order 4 Emission wavelength range 250 to 900 nm Zero order Photomultiplier must be changed at emission wavelength greater than 731 nm 5 Spectral bandwidth on excitation side 15 n...

Страница 46: ...e Tangent method b With emission side spectral bandwidth set at 30 nm S N ratio for Raman peak of water 900 or more Baseline method Wavelength 350 nm Time constant 2 0 s 14 Auto Zero range 0 to 1000 FLU 15 Offset range 0 to 1000 FLU settable in steps of 1 16 Spectrum memory Memorized contents Excitation and fluorescence spectra Number of spectra memorized 4 Measuring wavelength range Changeable in...

Страница 47: ...communication 22 External output terminal Option Processor 1 V Full scale Recorder 10 mV Full scale 23 External I O contact terminals Time Program Start Error Input Output Busy Output Above contacts are incorporated in e Line connector and effective with analog connection Auto Zero Input Lamp Off Input 24 Flow cell Standard 25 Flow cell capacity Standard 12 L irradiated capacity 26 Flow cell withs...

Страница 48: ...2 20 31 Dimensions 340 W mm 464 D mm 297 H mm 32 Weight Approx 26 7 kg NOTE Please contact your local Hitachi High Tech Science Corporation sales representative about a purchas of the Thermo cell ...

Страница 49: ...lay monitor for a long time take a break for 10 to 15 minutes per hour for health of your eyes and body 3 1 Basic Operation First turn on the power supply to display the 1st LCD monitor screen Spend one hour or more for warming up time to stabilize the xenon lamp The numerical value indicated before input is default values or currently set values 3 1 1 Power supply The power activation operation o...

Страница 50: ...arried out The current channel setting LED and the Xe LAMP LED will light in blue Initial screen FL Detector 8DD8440 00 Hitachi High Tech Science Corp 2017 Initializing screen WL DRIVE LAMP INITIALIZING The sequence of wavelength drive check of reference point for drive lighting of the lamp and auto zeroing is performed 3 The first monitor screen will be indicated after initialize processing is co...

Страница 51: ...lamp and OFF stands for the extinguished status d Fluorescence intensity FL is indicated within a range from 1000 0 to 1000 0 If the intensity is outside this range is indicated e Status indicators should be read as follows I Initial status R Time program running status B Busy status L Key locked status 4 Turn off the power switch of the detector Elapsed time Excitation wavelength Emission wavelen...

Страница 52: ...5s 3 4 1 0s 4 2 0s 5 4 0s 6 8 0s 7 d Program setup screen TIME Ex Em BASE PMT 0 0 250 350 1 3 1 Input procedure i The cursor is indicated on the front line of the input part Currently set values are indicated in the input part ii Numeric keys and decimal points are accepted by the upper limit Parameter name Input range Input part Parameter name Input item Input range Input part Parameter name Sele...

Страница 53: ... entering data and HAVE pressed the ENT key press the ESC key for return to the 1st monitor screen then re edit the value c Press the DEL key to delete the item in the input part while editing the program When the ESC key is pressed while setting plural parameters the item which the ESC key has pressed will be updated 3 For canceling the setup conditions press the ESC key for return to the 1st mon...

Страница 54: ...3 1 4 Wavelength setting Specify Ex Excitation wavelength and Em Emission wavelength 1 Press the WL key 2 The following display will be presented Set an excitation wavelength Ex cursor appears under Ex Ex wavelength setting screen Ex Em 200 850 0 nm 250 350 Press numeric keys 2 0 0 to 8 5 0 and then the ENT key This wavelength is settable within a range from 200 to 850 nm in increments of 1 nm Def...

Страница 55: ...ength 4 Wavelength setting is now completed and the 1st monitor screen returns At Ex and Em the set values are indicated 1st monitor screen TIME Ex Em FL PROG LAMP I 0 0 250 350 0 0 1 Xe 3 1 5 Shutdown procedure Turn the power supply of the detector off to shut it down The set measurement conditions are retained by the memory even when the power is switched off When power is turned on again the co...

Страница 56: ...put 1 Press the UTLTY SET UP key 2 The following display will be presented Utility initial screen UTILITY OFFSET 1 TIME CONSTANT 2 _1 USE PROG 3 PMT VOLT 4 OTHERS 5 Select OFFSET Press numeric key 1 and then the ENT key 3 The following display will be presented Offset setting screen OFFSET 0 1000 _ 0 Set an offset value Press numeric key 0 to 1 0 0 0 usually input 0 and then the ENT key An offset ...

Страница 57: ...rocessor see Fig 3 2 b Fig 3 2 Example of Offsetting 4 Offset setting is now completed and the 1st monitor screen returns Though no value is indicated for FL will be displayed when selecting a value other than 0 1st monitor screen TIME Ex Em FL PROG LAMP I 0 0 250 350 0 0 1 Xe 3 2 2 Time constant setting Specify electrical response time 1 Press the UTLTY SET UP key 2 The following display will be ...

Страница 58: ... is now completed and the 1st monitor screen returns 3 2 3 Use time program setting It is selectable whether or not to use automatic change of measurement parameters in response to measurement time time program 1 Press the UTLTY SET UP key 2 The following display will be presented Utility initial screen UTILITY OFFSET 1 TIME CONSTANT 2 _3 USE PROG 3 PMT VOLT 4 OTHERS 5 Select USE PROG Press numeri...

Страница 59: ...ogram is avoided is indicated for PROG 1st monitor screen TIME Ex Em FL PROG LAMP I 0 0 250 350 0 0 Xe 3 2 4 PMT voltage setting Set photomultiplier voltage for fluorescence detection 1 Press the UTLTY SET UP key 2 The following display will be presented Utility initial screen UTILITY OFFSET 1 TIME CONSTANT 2 _4 USE PROG 3 PMT VOLT 4 OTHERS 5 Select PMT VOLT Press numeric key 4 and then the ENT ke...

Страница 60: ...ing A bandwidth value of the emission monochromator is settable Standard is 15 nm and wide setting is 30 nm a Press the UTLTY SET UP key b The following display will be presented Utility initial screen UTILITY OFFSET 1 TIME CONSTANT 2 _5 USE PROG 3 PMT VOLT 4 OTHERS 5 Select OTHERS Press numeric key 5 and then the ENT key c The following display will be presented Others setting screen OTHERS Em BA...

Страница 61: ...or screen returns 2 ERROR OUT setting It is selectable whether to enable or disable the error signal output When enabled the following errors can be indicated Xenon lamp error ADC error DAC error Data buffer overflow CDS system Overheat Refer to Section 4 7 a Press the UTLTY SET UP key b The following display will be presented Utility initial screen UTILITY OFFSET 1 TIME CONSTANT 2 _5 USE PROG 3 P...

Страница 62: ...1 Invalid 0 or valid 1 status of error out signal output is settable Press numeric key 0 or 1 and then the ENT key Default value 1 e Error out setting is now completed and the 1st monitor screen returns 3 LEAK SENSOR setting Whether to use the liquid leak sensor or not is settable a Press the UTLTY SET UP key b The following display will be presented Utility initial screen UTILITY OFFSET 1 TIME CO...

Страница 63: ...n the ENT key Default value 0 When use ON 1 of the leak sensor is selected an error message will be indicated upon detection of liquid leakage Refer to Section 4 6 When power supply is restored setting will be use ON 1 of the leak sensor e Selection or avoidance of the leak sensor is now completed and the 1st monitor screen returns 4 LAMP setting ON or OFF status of the xenon lamp is selectable in...

Страница 64: ...non lamp Press numeric key 0 or 1 usually input 1 and then the ENT key Default value 1 e Xenon lamp setting is now completed and the 1st monitor screen returns When the xenon lamp is lit Xe is displayed for LAMP And when the lamp is extinguished OFF is displayed for LAMP 1st monitor screen TIME Ex Em FL PROG LAMP I 0 0 250 350 0 0 1 OFF The Xe lamp will light up when this unit is powered on NOTE A...

Страница 65: ...ar as desired is already indicated it must be reentered without fail 3 3 1 Xe lamp logbook data display Total on period of the Xe lamp and its standard lifetime in parentheses under Xe LAMP the number of switching on times and the date of the last lamp change can be indicated 1 Press the GLP key 2 The following display will be presented GLP initial screen GLP LOGBOOK 1 KEY LOCK 2 1 WL CHECK 3 Xe C...

Страница 66: ... change will be indicated Logbook report screen Xe LAMP SWITCHING LAST CHANGED DATE 123h 500 23 times 9 1 2010 Press the ENT or ESC key 6 Logbook display is now completed and the 1st monitor screen returns 3 3 2 Xe lamp logbook data resetting Total on period of the Xe lamp the number of switching on times and the date of the last lamp change are reset 1 Press the GLP key 2 The following display wi...

Страница 67: ... Xe lamp change date setting screen for setting the date of xenon lamp change 5 Set the date of xenon lamp change in the following procedure a Input a month for MM cursor appears under MM Xe lamp change date setting screen month Xe LAMP CHANGE MM DD YYYY 1 12 9 1 2010 Press numeric key 1 to 1 2 and then the ENT key The cursor moves to DD b Input a day for DD Xe lamp change date setting screen day ...

Страница 68: ...on period of the Xe lamp and the number of switching on times will be automatically reset accordingly 3 3 3 Hg lamp logbook data display Total on period of the Hg lamp the number of switching on times and the date of the last lamp change can be indicated 1 Press the GLP key 2 The following display will be presented GLP initial screen GLP LOGBOOK 1 KEY LOCK 2 1 WL CHECK 3 Xe CHECK 4 Select LOGBOOK ...

Страница 69: ...CHANGED DATE 12h 56 times 9 1 2010 Press the ENT or ESC key 6 Logbook display is now completed and the 1st monitor screen returns 3 3 4 Hg lamp logbook data resetting Total on period of the Hg lamp the number of switching on times and the date of the last lamp change are reset 1 Press the GLP key 2 The following display will be presented GLP initial screen GLP LOGBOOK 1 KEY LOCK 2 1 WL CHECK 3 Xe ...

Страница 70: ... procedure a Input a month for MM cursor appears under MM Hg lamp change date setting screen month Hg LAMP CHANGE MM DD YYYY 1 12 _9 1 2010 Press numeric key 1 to 1 2 and then the ENT key The cursor moves to DD b Input a day for DD Hg lamp change date setting screen day Xe LAMP CHANGE MM DD YYYY 1 31 9 1 2010 Press numeric key 1 to 3 1 and then the ENT key The cursor moves to YYYY c Input a year f...

Страница 71: ...splay will be presented GLP initial screen GLP LOGBOOK 1 KEY LOCK 2 2 WL CHECK 3 Xe CHECK 4 Select KEY LOCK Press numeric key 2 and then the ENT key 3 The following display will be presented Key lock selecting screen KEY LOCK YES 1 NO 0 1 Set selection 1 or avoidance 0 of KEY LOCK Press numeric key 1 or 0 usually input 1 and then the ENT key Default value 0 Even with the keys locked the monitor sc...

Страница 72: ...appears and it is checked whether wavelength is normal or not WL accuracy checking screen running CHECKING WL ACCURACY If an Hg lamp lighting error occurs the error message shown below will appear Return to the 1st monitor screen by pressing the CL key Hg lamp error screen Hg LAMP ERROR PRESS CL KEY TO CLEAR MESSAGE 4 The result of excitation wavelength Ex check will be displayed automatically Ex ...

Страница 73: ...e ENT or ESC key the system will return automatically to the 1st monitor screen 6 When the wavelength accuracy check is finished the system returns to the 1st monitor screen 3 3 7 Xe lamp energy check Energy level of the xenon lamp can be checked using the xenon lamp beam at 254 nm 1 Press the GLP key 2 The following display will be presented GLP initial screen GLP LOGBOOK 1 KEY LOCK 2 4 WL CHECK ...

Страница 74: ...pses without pressing ENT or ESC key the system will return automatically to the 1st monitor screen 5 When the Xe lamp energy check is finished the system returns to the 1st monitor screen NOTE When the temperature of the Xe lamp is high the sealed gas pressure will fluctuate and the lamp may be difficult to ignite Before restarting the instrument or re lighting the lamp wait at least five minutes...

Страница 75: ...y to go back to the preceding step or the key to advance to the next step When the setting at each step is finished press the ESC key to return to the 1st monitor screen and the time program is registered NOTE When setting the time program be sure to set the TIME for the first step at 0 0 This cannot be changed or deleted And for the final step of the program be sure to set the measurement complet...

Страница 76: ...creen appears 0 is always indicated here Mode setting screen EDIT MODE MODIFY 0 NEW 1 1 Press a numeric key 0 or 1 and then the ENT key to either use MODIFY 0 or delete NEW 1 the conditions in the time program No selected above 0 Conditions in the time program No selected above are used additions or changes can be made in the program 1 Conditions in the time program No selected above are deleted a...

Страница 77: ... the bottom right of the screen The cursor now moves to Ex b Set an Ex excitation wavelength Step setting screen Ex indicated TIME Ex Em BASE PMT 0 0 250 1 Press numeric keys 2 0 0 to 8 5 0 and then the ENT key Setting range 200 to 850 nm It is recommended to set the same wavelength as was set for Ex of the measuring wavelengths Initial default value blank The cursor next moves to Em c Set an Em e...

Страница 78: ...RO is executed Data baseline is set to the zero point of the recorder 2 HOLD is executed Baseline is held so that it doesn t fluctuate HOLD function is released by executing AUTO ZERO Initial default value 1 e Set the PMT photomultiplier voltage Step setting screen PMT indicated TIME Ex Em BASE PMT 0 0 250 350 1 3 1 Press a numeric key 1 to 5 and then the ENT key f When the setting for the first s...

Страница 79: ...crements of 0 1 minute The cursor now moves to Ex b Set an Ex excitation wavelength to be changed Step setting screen Ex indicated TIME Ex Em BASE PMT 10 0 400 1 1 Press numeric keys 2 0 0 to 8 5 0 and then the ENT key The cursor next moves to Em c Set an Em emission wavelength to be changed Step setting screen Em indicated TIME Ex Em BASE PMT 10 0 400 500 1 Press numeric keys 2 5 0 to 9 0 0 and t...

Страница 80: ... 1 Press a numeric key 1 to 5 and then the ENT key f When the setting for the second step is completed return to the TIME setting on step setting screen If more parameters will be changed then repeat the operation in this subsection If parameters will not be changed advance to the final step setting 6 Setting of final step of time program a Set the measurement end TIME Step setting screen TIME ind...

Страница 81: ...e program starting Press the START key to start the time program Status indication on the 1st monitor screen becomes R time program is running and the elapsed time is indicated at TIME The PROG RUN LED lights up in blue 1st monitor screen TIME Ex Em FL PROG LAMP R 250 350 0 0 1 Xe 3 4 3 Time program stopping Press the STOP key then the time program under execution stops and the 1st monitor screen ...

Страница 82: ... photomultiplier voltage is changed from MID to LOW excitation wavelength from 250 to 270 nm emission wavelength from 350 to 370 nm and hold function is applied then measurement is completed after 20 minutes 2 250 350 270 370 ON ON 3 2 0 0 10 0 20 0 START STOP Ex nm Em nm A Z HOLD PMT Auto zero HOLD TIME minutes ...

Страница 83: ... and then the ENT key TIME Ex Em BASE PMT 0 0 250 350 1 3 1 Press 1 0 keys and then the ENT key Press 2 7 0 keys and then the ENT key Press 3 7 0 keys and then the ENT key Press 2 key and then the ENT key Press 2 key and then the ENT key TIME Ex Em BASE PMT 10 0 270 370 2 2 1 Press 2 0 keys and then the ENT key TIME Ex Em BASE PMT 20 0 1 Press the ESC key Time program setting is now finished TIME ...

Страница 84: ...he ENT key EDIT MODE MODIFY 0 NEW 1 0 Press 0 key and then the ENT key Step at startup is indicated TIME Ex Em BASE PMT 0 0 250 350 1 3 1 Press key to access the next step TIME Ex Em BASE PMT 10 0 270 370 2 2 1 Press key to access the next step TIME Ex Em BASE PMT 20 0 1 Press the ESC key and the 1st monitor screen returns TIME Ex Em FL PROG LAMP I 0 0 250 350 0 0 1 Xe ...

Страница 85: ...t more than 30 nm apart for measurement NOTE The maximum wavelength scan range from end to start wavelength for both excitation and emission is 400 nm For spectrum acquisition in a range greater than 400 nm the range should be divided into two for measurement NOTE Saved spectral data will not be retained when the power is turned OFF 1 Measurement and Recording of Spectrum for Excitation Ex Scan Ra...

Страница 86: ...1 WL2 nm FL Ex 250 600 200 600 Press numeric keys 2 5 0 to 8 5 0 and then the ENT key Input value Value of less than 850 within WL1 50 to WL1 400 Setting range 250 to 850 nm Initial default value 600 Pressing key prior to the ENT key will return to WL1 e A memorize spectrum setting screen will now appear Memorize spectrum setting screen MEMORIZE SPECTRUM NO 1 4 1 Set a memorize spectrum number for...

Страница 87: ...t above g When the spectral data memorization is completed the system returns to the 1st monitor screen automatically 2 Measurement and Recording of Spectrum for Emission Em Scan Range a Press the SPECT key b The scan mode setting screen will appear Scan mode setting screen SPECTRUM MODE FL Ex 1 FL Em 2 2 Press numeric key 2 and then the ENT key to select FL Em Now proceed to the scan range settin...

Страница 88: ... 400 Setting range 300 to 900 nm Initial default value 700 Pressing key prior to the ENT key will return to WL1 e A memorize spectrum setting screen will now appear Memorize spectrum setting screen MEMORIZE SPECTRUM NO 1 4 1 Set a memorize spectrum No for the spectral data Press a numeric key 1 to 4 and then the ENT key Initial default value blank Upon entering a memorize spectrum No the previous ...

Страница 89: ...emission due to solvent or impurities in it and second or third order emission may be superimposed on the emission of the analyte element Similarly an excitation spectrum may be affected by scattered light at the emission wavelength by Raman scattering due to solvent by emission due to solvent or impurities contained in it and by second or third order scattered light of the excitation beam These f...

Страница 90: ...l scale range setting screen RCD RANGE 1 1000 1000 Press numeric keys 1 to 1 0 0 0 and then the ENT key to set a recorder full scale range Initial default value 1000 d When the setting of recorder full scale range is finished the system returns to the 1st monitor screen 2 Setting of Recorder Output Speed a Press the RECORD key b The recorder setting screen appears Recorder setting screen RECORD RC...

Страница 91: ...E 1 RCD SPEED 2 3 SPECT RCD 3 Press numeric key 3 and then the ENT key to select SPECT RCD spectral recording c The output data spectrum No setting screen appears Output data spectrum No setting screen RECORD SPECTRUM NO 1 4 1 Press a numeric key 1 to 4 and then the ENT key to set a spectrum No to be output Initial default value 1 d The background spectrum No setting screen now appears The backgro...

Страница 92: ...ssage indicated below will appear Press the CL key and the 1st monitor screen will return Error display SPECTRA ARE MISMATCHED PRESS CL KEY TO CLEAR MESSAGE e The ready for output monitor screen will appear Ready for output monitor screen RCD NO SPEED WL RANGE Em WIDTH STD 1 60 200 600 PRESS START Press the START key Pressing the ESC key prior to the START key will cancel the process The output sp...

Страница 93: ...r screen will return automatically 3 4 7 Marker operation A marker line is indicated only on the recorder It is usable as an event marker to indicate for example the start of measurement or the point where set conditions were changed during measurement Press the 1 MARKER key to indicate a marker line on the recorder data ...

Страница 94: ...n on the power switch with the UTLTY SET UP key held down 2 The following display will be presented Setup initial screen SETUP DETECT THERMO CONTRAST S NO CH1 NO USE 4 02AB 111 Press the ENT key 3 The following display will be presented Setup items selecting screen SETUP ITEMS DETECTOR 1 CONTRAST 2 1 EXCHANGE STANDARD 3 Select DETECTOR Press numeric key 1 and then the ENT key 4 The following displ...

Страница 95: ...s indicated Setup initial screen SETUP DETECT CONTRAST S NO CH1 4 02AB 111 When you press the ENT key here the setup items selecting screen is presented to allow reentry Or you can advance to LCD contrast setting The selected setting will be fetched upon turning on power supply at the next time 6 Turn off the power switch ...

Страница 96: ...ems selecting screen SETUP ITEMS DETECTOR 1 CONTRAST 2 2 EXCHANGE STANDARD 3 Select CONTRAST Press numeric key 2 and then the ENT key 4 The following display will be presented LCD contrast setting screen LCD CONTRAST 1 7 3 Set a LCD CONTRAST value Press a numeric key 1 to 7 usually input 3 and then the ENT key 1 Dark 7 Bright Default value 4 5 LCD contrast setting is now completed and the setup in...

Страница 97: ...e will rise abnormally to cause a danger of burst etc Replace the xenon lamp with a new one before its cumulative turn on time exceeds the guaranteed useful lifetime The standard life time of the xenon lamp is settable This setting activates the following function If the total operation time of the Xe lamp is longer than the standard lifetime upon turning on the lamp an error message is indicated ...

Страница 98: ...ame Guaranteed Life Limit Allowable Input 650 1500 150 W Xe lamp 150 hours 500 hours 500 or less 890 2019 Long life Xe lamp 500 hours 1 000 hours 1000 or less Default value 500 hours 650 1500 Xe lamp is attached with shipment 5 The setting of a standard Xe lamp lifetime is now completed and the following display will be presented Setup initial screen SETUP DETECT CONTRAST S NO CH1 3 02AB 111 When ...

Страница 99: ...top operation immediately Leakage of solvent or waste solution Leakage of solvent inside the instrument When using flammable chemicals be careful about possible ignition due to static electricity To prevent the build up of static electricity use a conductive container for waste solution and provide proper grounding connection to it WARNING Explosion of Vapor from Flammable Chemicals If a flammable...

Страница 100: ...teristics of Organic Solvents 2 When performing high sensitivity analysis with a UV detector use solvents whose UV absorption characteristics are especially small Use solvents for liquid chromatograph commercially available Absorption characteristics of special grade reagents such as methanol ethanol and acetonitrile generally and widely used are greatly different in the short wavelength range dep...

Страница 101: ...hich contain halogen ions such as HCl KCl NaCl NH4Cl are typical reagents Reagents which may be restricted are shown in Table 4 1 use the table as a rough standard NOTE When unsuitable reagents absolutely necessary for ingredient separation or reaction of a constituent have been used or when mobile phase which deposits salt has been used wash all the flow paths with distilled water after completin...

Страница 102: ...lorate Ammonium phosphate Boric acid Formic acid up to pH3 Hydrochloric acid up to pH3 Potassium nitrate Sodium hydrogencarbonate Sodium carbonate Ammonium chloride Potassium chloride Sodium chloride Tolufluoroacetic acid Note 1 Max concentration of above reagents should be 5 6 Note 2 When the above reagents have been used necessarily wash all the flow paths with distilled water completely without...

Страница 103: ...n abnormality is found stop operation immediately Leakage of solvent or waste solution Leakage of solvent inside the instrument When using flammable chemicals be careful about possible ignition due to static electricity To prevent the build up of static electricity use a conductive container for waste solution and provide proper grounding connection to it WARNING Explosion of Vapor from Flammable ...

Страница 104: ...n or injury For details of the properties of each solvent and how to handle it refer to the relevant Safety Data Sheets SDS Be sure to handle each solvent properly a Wear proper protective clothes e g safety goggles so that a solvent will not come into direct contact with the skin b Ventilate the laboratory room adequately to prevent accidental inhalation of harmful solvent vapor ...

Страница 105: ... 37 8 1 4 Ethyl ether 0 38 1 353 220 45 180 1 9 48 2 6 35 0 7 Ethyl sulfide 0 38 0 23 1 442 290 Chloroform 0 40 0 45 1 443 245 Methylene chloride 0 42 0 57 1 424 245 50 518 9 3 8 15 4 2 2 38 5 0 9 Methyl i butyl ketone 0 43 0 44 1 394 330 Tetrahydrofurane 0 45 1 408 220 14 4 321 1 2 11 8 2 5 66 1 0 9 Ethylene dichloride 0 49 1 445 230 13 3 412 3 6 2 16 3 4 83 9 1 3 Methyl ethyl ketone 0 51 0 79 1 ...

Страница 106: ...Use solvents whose boiling point is 55 C or higher Solvents of low boiling point are not only dangerous but also generate bubbles when sucked by pump so that normal pumping will be impossible 3 When performing high sensitivity analysis with UV detector use solvents which absorb especially small amount of UV light Since special grade reagents such as methanol and acetonitrile generally used absorb ...

Страница 107: ...onductive container for waste solution and provide proper grounding connection to it Since flammable organic solvents are used for the high speed liquid chromatograph be careful about heat and fire Since thin tubings are used for the liquid chromatograph static electricity is easily generated If charged liquid is collected into an insulated waste container for example and an electric discharge occ...

Страница 108: ...es d Variation of pump pressure e Reduced precision of gradient mixing ratio 2 For degassing mobile phase two methods are available Degassing unit and Manual Degassing method is explained in 3 7 2 3 Use mobile phase without dust Dust in mobile phase causes trouble in pumping and the pressure may vary 3 7 2 Degassing mobile phase There are various ways of degassing Perform degassing suitable for th...

Страница 109: ... generated first then gradually larger bubbles are generated Continue degassing until bubbles are not generated anymore It takes 5 to 10 minutes to finish d When bubble generation finishes turn off the power of ultrasonic cleaner e Take out mobile phase container from ultrasonic cleaner wipe the water from the container and set the container to liquid chromatograph 2 Example of Degassing by Reduci...

Страница 110: ...trasonic cleaner with water and aspirator to decrease pressure Fix the degassing time b Insert rubber tube of aspirator into mouth of reagent bottle c Turn on the power of ultrasonic cleaner d Open the faucet of city water for aspirator to decrease pressure e When degassing small bubbles are generated first then gradually larger bubbles are generated Continue degassing until bubble generation fini...

Страница 111: ...tion ratio of mobile phase may be varied by degassing time boiling point of mobile phase would vary with a reduced pressure 3 7 3 Removing dust from mobile phase There are various ways to remove dust from mobile phase Remove dust in the way suitable for the purpose of use 1 Example of Filtering of Mobile Phase by Using Membrane Filter Removing dust and degassing can be performed simultaneously by ...

Страница 112: ...lity of data e g generating abnormal peaks etc a Example of filtering samples Dust in sample can be removed easily as follows Take sample in Syringe attach cartridge type 0 45 µm membrane filter and perform filtering See the figure left NOTE There are various ways for filtering sample Filtering sample in the way suitable for the sample amount Filtering by cartridge type 0 45 µm membrane filter Fil...

Страница 113: ...lumn foreign matters can be removed as follows Take sample in Syringe attach Normal Phase cartridge and perform filtering See the figure left NOTE There are various ways for removing foreign matters in sample Remove in the way suitable for the purpose of analysis Cartridge of silica gel Clean sample after removing foreign matters ...

Страница 114: ...rformance Positional adjustment of the lamp is necessary for an accurate performance check NOTE When this detector unit is used in combination with Primaide System Manager ChromAssist Data Station the lamp energy wavelength accuracy and sensitivity can be automatically calculated For details refer to the instruction manual of Primaide System Manager ChromAssist Data Station 4 2 1 Xe lamp energy ch...

Страница 115: ...red for the measurement The following items are required Pump Recorder or D 2500 chromato data processor Distilled water 1 Injection of Distilled Water Connect tubing as shown in Fig 4 1 supply distilled water at a flow rate of 1 mL min for 3 minutes with the Primaide 1110 Pump in Manual mode then set the flow rate to 0 and stop the supply of water Remove the flow cell unit and check for any bubbl...

Страница 116: ... nm Time constant response 2 seconds Photomultiplier voltage MID Emission spectrum bandwidth STANDARD 15 nm or WIDE 30 nm Emission spectrum measuring wavelength 350 to 450 nm Spectrum No Any of 1 to 4 a Turn ON the power switch and the 1st monitor screen appears b Set an excitation wavelength i Press the WL key and the wavelength setting screen appears Set an excitation Ex wavelength Primaide 1440...

Страница 117: ... a photomultiplier voltage and an emission spectrum bandwidth i Press the UTLTY SET UP key and the utility setting screen appears Utility setting screen UTILITY OFFSET 1 TIME CONSTANT 2 _2 USE PROG 3 PMT VOLT 4 OTHERS 5 Press numeric key 2 and then the ENT key ii The time constant setting screen appears Time constant setting screen TIME CONSTANT 0 05s 1 0 1s 2 0 5s 3 5 1 0s 4 2 0s 5 4 0s 6 8 0s 7 ...

Страница 118: ...and the utility setting screen reappears Utility setting screen UTILITY OFFSET 1 TIME CONSTANT 2 5 USE PROG 3 PMT VOLT 4 OTHERS 5 Press numeric key 5 and then the ENT key viii The others setting screen appears Others setting screen OTHERS Em BANDWIDTH 1 ERROR OUT 2 1 LEAK SENSOR 3 LAMP 4 Press numeric key 1 and then the ENT key ix The Em bandwidth setting screen appears Em bandwidth setting screen...

Страница 119: ...for WL1 Scan range setting screen SCANNING WL RANGE WL1 WL2 nm FL Em 250 850 350 700 Press numeric keys 3 5 0 and the ENT key Enter an end wavelength maximum value for WL2 Scan range setting screen SCANNING WL RANGE WL1 WL2 nm FL Em 400 750 350 450 Press numeric keys 4 5 0 and the ENT key iii The memorize spectrum No setting screen appears Memorize spectrum No setting screen MEMORIZE SPECTRUM NO 1...

Страница 120: ... RECORD RCD RANGE 1 RCD SPEED 2 1 SPECT RCD 3 Press numeric key 1 and then the ENT key ii The recorder full scale setting screen appears Recorder full scale setting screen RCD RANGE 1 1000 1000 Press numeric keys 1 0 0 0 and then the ENT key iii The 1st monitor screen returns iv Press the RECORD key And the recorder setting screen appears Recorder setting screen RECORD RCD RANGE 1 RCD SPEED 2 2 SP...

Страница 121: ...O 1 4 1 Press a numeric key 1 to 4 and then the ENT key Set the same number as was entered when making the setting for spectrum measurement ix The background spectrum No setting screen appears Background spectrum No setting screen BACKGROUND SPECTRUM NO 0 4 0 Press numeric key 0 and then the ENT key x The output ready monitor screen appears Output ready monitor screen RCD NO SPEED WL RANGE Em WIDT...

Страница 122: ...E If the detector and data processor are connected via E Line digital start the output by pressing the ALL START key of the data processor 3 Determination of Raman Signal Intensity Fig 4 2 Measuring the Raman Signal Intensity Determine the voltage value corresponding to S in the figure When using the D 2500 chromato data processor calculation can be made using the length L in mm of S on the chart ...

Страница 123: ...rd the baseline A suitable attenuator setting is ATT 0 when using the D 2500 Drift can also be measured if the measurement is continued for 15 minutes or longer NOTE In measurement with the emission spectrum bandwidth set at WIDE 30 nm the peak emission wavelength is shifted toward the shorter wavelength side from 397 nm Due to the WIDE setting the spectrum bandwidth widens for the scattered light...

Страница 124: ...minute Fig 4 3 Noise Calculation extracted from JAIMAS 0005 2005 NOTE The Raman spectrum of water and the noise level may be affected by the purity of the water used and or the presence of air bubbles 7 Measurement of Raman Spectrum of Water Using a Time Program when Raman signal intensity obtained in the preceding subsection 2 is low If the sensitivity S N ratio of Raman peak of water is low rech...

Страница 125: ...bubbles may remain in the cell To prevent this first inject ethanol or the like and then water iii Change the method of spectrum measurement via time program Measure the Raman spectrum of water using a time program But first check the fluctuation of FL value via wavelength shift Set the excitation Ex wavelength at 350 nm and emission Em wavelength at 450 nm using the WL key Then zero the FL value ...

Страница 126: ... 5 415 1 6 430 1 8 450 2 0 NOTE The above time program shows an example where the minimum FL value was obtained at 380 nm and the maximum at 400 nm For the top Em of the program set a wavelength that includes the wavelengths where the maximum and minimum FL values were obtained For instance if the maximum FL value was obtained at a wavelength of 397 nm then the wavelength at 1 1 minutes should be ...

Страница 127: ... Refer to previous subsections 4 through 6 for measurement and calculation of noise and calculation of S N ratio 8 Measurement of Drift Drift is measured by using the data and baseline obtained from the Raman spectrum of water measurement conditions are the same as in preceding subsection 4 but measuring time is 15 minutes For the sake of simplicity calculation can be performed on the assumption t...

Страница 128: ...amp As for the wavelength accuracy of the excitation side monochromator the Em side is fixed at the wavelength position where the abovementioned bright line was detected then the Ex wavelength where a Rayleigh scattering peak of 254 nm is produced is measured D Raman spectrum of water S tangent method Baseline baseline method A S Max wavelength 450 nm Emission wavelengths Baseline emission wavelen...

Страница 129: ...ration 2 Calibration of Wavelength Accuracy If there is a wavelength deviation then adjust the wavelength in the wavelength calibration mode special mode a Calibration of excitation wavelength i Press the UTLTY SET UP key and the utility setting screen appears Utility setting screen UTILITY OFFSET 1 TIME CONSTANT 2 99 USE PROG 3 PMT VOLT 4 OTHERS 5 Press numeric keys 9 9 and then the ENT key ii Th...

Страница 130: ...E PROG 3 PMT VOLT 4 OTHERS 5 Press numeric keys 9 8 and then the ENT key ii The emission wavelength calibration mode screen appears Emission wavelength calibration mode screen Em WL ADJUST 1 20 10 ONLY FOR FACTORY USE 2 The present offset value is indicated at Em WL ADJUST Indicated at the bottom right of the screen is the wavelength check result deviation of wavelength indication range of 10 to 1...

Страница 131: ...eak 1 While a solvent is used the leak sensor function should be kept active 2 The leak sensor function of this instrument may not work when liquid leaks in a small amount Leakage of a highly volatile solvent may not be detectable either Even when the leak sensor function is activated you should be careful not to overlook liquid leakage for avoiding a serious situation 3 As a routine checkup visua...

Страница 132: ...g to the liquid leak sensor and drain tray and then fasten the sensor to the drain tray again NOTE While cleaning the liquid leak sensor be careful not to damage its tip If damaged the liquid leak sensor may not work properly or an error may occur Fig 4 7 Liquid Leak Sensor Fastening Screw NOTE Mount the leak sensor at the correct position If the sensor is mounted at a wrong position it may not wo...

Страница 133: ...ent will not come into direct contact with the skin b Ventilate the laboratory room adequately to prevent accidental inhalation of harmful solvent vapor Refer to the following procedure for the drain tray check 1 Remove retaining screws to detach the drain tray NOTE Avoid breakage of the detached leak sensor to prevent malfunction 2 Pull out the drain tray from the unit 3 Wipe up the drain tray wi...

Страница 134: ...curacy Yearly Check wavelength by using bright line of Hg lamp at 254 nm See 4 2 4 NOTE First adjust the xenon lamp position see 5 2 1 3 then make an energy check of the xenon lamp and a wavelength accuracy check using the Hg lamp Correct measurement results will not be obtained unless the lamp position is adjusted 4 3 1 Xe lamp energy check Check the energy level of the Xe lamp at a wavelength of...

Страница 135: ...gth calibration is required Refer to section 4 2 4 2 for the calibration 4 3 3 Lamp logbook check The cumulative turn on time number of lightings and last replacement date for the Xe and Hg lamps can be checked via the GLP function see 3 3 ...

Страница 136: ... or waste solution Leakage of solvent inside the instrument When using flammable chemicals be careful about possible ignition due to static electricity To prevent the build up of static electricity use a conductive container for waste solution and provide proper grounding connection to it WARNING Explosion of Vapor from Flammable Chemicals If a flammable chemical such as organic solvent leaks from...

Страница 137: ... flow cell unit straight out 3 Check the inside of the cell visually to see if it is contaminated with any foreign substance crystallized substance etc 4 Check the outside of the cell for any leakage 5 If contamination is observed inside the cell it must be cleaned with an appropriate solution If an aqueous solvent has been used distilled water should be used for washing And if an organic solvent ...

Страница 138: ...ace the wash fluid with the mobile phase that is used for separation and repeat the procedure in step 5 7 If air bubbles are observed in the flow cell supply solution into the cell with the pump at a flow rate within 1 0 mL min and a pressure within 1 0 MPa While the solution is being supplied into the flow cell pinch the end of the drain tube with your fingers for a few seconds to increase the pr...

Страница 139: ...and cleaning of flow cell 1 Disassembly of Flow Cell If contamination is not removed by just flushing the interior of the flow cell then disassemble and clean the cell in the procedure below Fig 4 11 Flow Cell Unit a Loosen retaining screws 1 and 2 and detach the shielding plate b Detach the cell window Shielding plate Retaining screw 1 Retaining screw 2 Setscrew 1 Setscrew 2 From the pump ...

Страница 140: ...container and then place it in the ultrasonic cleaner so as to avoid scratching the cell b Wipe the surface of the flow cell with gauze that is moistened with acetone or ethanol NOTE Be careful not to scratch the flow cell surface when disassembling it NOTE Make sure to put the flow cell in a paper or plastic container when cleaning it The use of a glass container may scratch the cell or preclude ...

Страница 141: ... cell surface when assembling it NOTE Take care about the orientation of the polished surfaces of the flow cell when putting it into the cell holder see Fig 4 13 A mistake in the orientation may preclude obtaining the proper performance from the instrument NOTE Tighten setscrew 1 fully with hand and then tighten setscrew 2 about 30 manually or tighten setscrew 1 further 30 with a wrench Excitation...

Страница 142: ...ormally setscrew tightening may be slightly inadequate Retighten setscrew 1 lightly about 10 to 20 with a wrench or setscrew 2 lightly about 10 to 20 manually 4 Wipe up any liquid spilt on the outside of the flow cell 5 Before returning the flow cell unit into position supply liquid with the pump and make sure there is no more leakage NOTE It is important that the OUTLET tube of the flow cell be c...

Страница 143: ...han a few days clean the flow cell interior thoroughly by pumping distilled water or alcohol through it and then store it in a safe place If the period of nonuse is less than a few days the inside of the cell may be filled completely with a solvent such as ethanol or acetonitrile and then stored ...

Страница 144: ...ck the monitor screen Press the ESC key The READY status will be presented The xenon lamp does not light The xenon lamp is still hot Turn on the lamp again For turning on the lamp again wait at least 5 minutes after the Xe lamp went out The Xe lamp has reached the end of its useful life Check the cumulative turn on time of Xe lamp Replace the Xe lamp with a new one Excessive noise The flow cell is...

Страница 145: ...tion Check lamp energy level Adjust lamp position 5 2 1 Error in flow cell mounting Visual check Correctly mount flow cell 4 4 Flow cell surface scratched Visual check Replace flow cell 4 4 Poor wavelength accuracy Water is not injected in the flow cell Inject water into the flow cell Bubbles have entered the flow cell Visual check Remove bubbles 4 4 Poor adjustment of xenon lamp position Check la...

Страница 146: ... 1st monitor screen returns 2 Turn on power again 3 Contact your nearest service office of Hitachi High Tech Science sales representative Ex Em SIDE OF WL DRIVE MECHANISM ERROR Failure in detecting Ex or Em wavelength origin during self diagnosis test initialization 1 Cannot be released by key operation 2 Turn on power again 3 Contact your nearest service office of Hitachi High Tech Science sales ...

Страница 147: ...IME PRESS CL KEY TO CLEAR MESSAGA The cumulative turn on time of xenon lamp has exceeded its standard exchange time 1 Press CL key and 1st monitor screen returns 2 Replace the lamp with a new one A Z OVER RANGE PRESS CL KEY TO CLEAR MESSAGE Allowable auto zero range has been exceeded 1 Press CL key and 1st monitor screen returns 2 Change the wavelength 3 Replace the solvent with another one 4 Clea...

Страница 148: ...ce sales representative DAC ERROR Data transfer to DAC has been interrupted 1 Cannot be released by key operation 2 Turn on power again 3 Contact your nearest service office of Hitachi High Tech Science sales representative An error message will be displayed together with the alarm buzzer sounding NOTE An input value stored in this instrument may be deleted if a certain error message appears In th...

Страница 149: ...elivery to customer 890 2019 Long life xenon lamp Special accessory Guaranteed life 500 hours Limit 1000 hours Warranty period 6 months after delivery to customer 890 2662 Mercury lamp Guaranteed life 100 hours 2 years J821399 Time lag fuse 5 A 890 2932 Flow cell 12 L 9600 hours 5 years 890 2684 Drain tube ID 0 33 0 62 T 9600 hours 5 years Length 2 m Outside diameter 1 57 mm 893 5860 Inlet tube ID...

Страница 150: ...mber Part Name Estimated Service Life Remarks 8DD 9109 Ferrule SUS 9600 hours 5 years Example use Connecting pipe 893 0825 Fitting 9600 hours 5 years Example use Column outlet connection For outer diameter 1 57 mm ...

Страница 151: ...ching the hot xenon lamp or its housing could result in severe burns Before proceeding to lamp replacement turn off the power switch and then wait for at least one hour until the lamp and its housing cool down sufficiently For replacing the xenon lamp immediately after the use the lamp must be cooled first of all because it is still hot Once the Xe lamp has been cooled adequately you can proceed t...

Страница 152: ...d then the ENT key c The Xe lamp setting screen appears Xe lamp setting screen Xe LAMP ON OFF OFF 0 ON 1 0 Press numeric key 0 and then the ENT key d The 1st monitor screen appears and OFF is indicated at LAMP 1st monitor screen TIME Ex Em FL PROG LAMP I 0 0 250 350 0 0 1 OFF e The xenon lamp is thus turned off Although the lamp is off the fan continues rotating so as to cool down the lamp Wait fo...

Страница 153: ... Injury due to Xenon Lamp Explosion If a strong shock or impact is applied to the xenon lamp or if the surface of its quartz glass part is scratched it may explode and scatter glass pieces resulting in personal injury Be sure to wear proper protective gear such as safety goggles safety mask thick long sleeves and gloves when handling the xenon lamp a Turn OFF the power switch and unplug the power ...

Страница 154: ... main body and loosen the lamp house retaining screw Fig 5 2 Removal of Connector and Retaining Screw e Lift the grip of the lamp house lightly and pull it toward you then detach the lamp house from the main body Fig 5 3 Removal of Lamp House Lamp house Retaining screw Grip Connecter Lamp house Grip ...

Страница 155: ...ed cotton cloth completely and crush its glass part with a hammer or the like Then discard the xenon lamp as a dangerous waste item properly If the xenon lamp is discarded without being crushed it could explode due to possible impact at the time of disposal scattering glass pieces to cause personal injury WARNING Injury due to Xenon Lamp Explosion at mounting When loosening or tightening the retai...

Страница 156: ...at a thin metal wire is attached near the center of the bulb which facilitates ignition of the lamp Take care not to break this wire when handling the lamp g Loosen the cathode nut and remove the cathode lead plate Fig 5 5 Fig 5 5 Detachment of Xenon Lamp Anode cord Anode nut Metal fixture Cathode nut Cathode lead plate Lamp bulb Ceramic plate Cathode cord Lamp protrusion Metal wire ...

Страница 157: ...enon lamp in the specified direction If the mounting direction polarity is wrong the cathode will be consumed significantly to disable turn on of the lamp Mount the lamp so that the anode mark on it will be positioned at the support metal of the lamp holder If the lamp with its cathode consumed excessively is used continuously pressure inside the lamp bulb may become too high causing possible expl...

Страница 158: ...nt front panel Fig 5 7 Be careful not to touch the lamp bulb Note that a thin metal wire is attached near the center of the bulb which facilitates ignition of the lamp Take care not to break this wire when handling the lamp Also if the thin metal wire is not located on the side of the lamp having the small protrusion slide the wire so it is properly positioned there Fig 5 6 The Xenon Lamp New xeno...

Страница 159: ...n the bulb faces the instrument front panel Fig 5 4 A mistake in the orientation may cause an increased light loss and preclude obtaining the desired performance from the instrument j After mounting the Xe lamp in the lamp house assemble it into the main body Fig 5 2 Raise the lamp house and insert it into the main body then lower it and press it downward into place The lamp house will not move if...

Страница 160: ...ectly when it is lit Be sure to wear tinted safety glasses to prevent possible eye damage The position of the beam slightly differs from one lamp to another so it is necessary to adjust the lamp position before the detector is first used or when the lamp is replaced Use the three adjusting screws on the right side of the instrument for the adjustment HORIZONTAL For adjusting horizontal position FO...

Страница 161: ...ment a Coarse adjustment Set the excitation Ex wavelength at 550 nm and make the adjustment while observing the image of the light beam Take the following steps for the adjustment see section 3 1 4 HORIZONTAL VERTICAL FOCUS Lamp position adjustment screw cover Cover fastening screw ...

Страница 162: ...reen appears 1st monitor screen TIME Ex Em FL PROG LAMP I 0 0 250 350 0 0 1 Xe ii In the present status pull out the flow cell unit see Fig 5 2 will be indicated at FL on the 1st monitor screen 1st monitor screen TIME Ex Em FL PROG LAMP I 0 0 250 350 1 Xe ...

Страница 163: ... monitor screen appears 1st monitor screen TIME Ex Em FL PROG LAMP I 0 0 550 350 1 Xe vi In this status place a paper strip at the Ex beam exit center of the sample compartment as shown in Fig 5 9 and observe the condition of the excitation beam Repeat the VERTICAL HORIZONTAL and FOCUS adjustments in this order little by little until the excitation beam rainbow color becomes brightest The coarse a...

Страница 164: ... given below refer to sections 3 1 1 3 1 4 i Re mount the flow cell unit ii Press the WL key and the wavelength setting screen appears Set an excitation Ex wavelength Wavelength setting screen Ex indicated Ex Em 200 850 0 350 350 Press numeric keys 3 5 0 and then the ENT key iii Emission Em wavelength will not be set here Leave the present setting as it is Observe excitation beam on paper strip ...

Страница 165: ...il this value is maximum When a maximum value is reached the fine adjustment of the lamp position is completed vii Press or ESC key and the 1st monitor screen will return 4 Entry of Lamp Replacement Date When the above procedure is finished enter a lamp replacement date see 3 3 2 With this entry the cumulative on time is set to 0 and the number of lightings to 1 5 2 2 Hg lamp replacement Item to p...

Страница 166: ... screw retaining the mercury lamp cover Fig 5 12 Fig 5 12 Removal of Hg Lamp Cover d Pull the Hg lamp cover straight out and the Hg lamp will be seen attached at the right side interior of the main body Fig 5 13 Hg lamp cover Retaining screw Retaining screws ...

Страница 167: ... the two retaining screws then connect the connector with the main body g Reattach the flow cell unit and light source cover in their original positions 2 Entry of Lamp Replacement Date When the above procedure is finished enter a lamp replacement date see 3 3 4 With this entry the cumulative on time is set to 0 and the number of lightings to 1 Close up view Connector Hg lamp Retaining screws ...

Страница 168: ...er cord before proceeding to fuse replacement Item to prepare Item to prepare 2 new fuses P N J821399 T5AH 1 Items to Check Prior to fuse replacement make sure the power switch of the instrument is off and the power cord is unplugged 2 Replacement of Fuse a Hold the tabs on both sides of the fuse holder and detach the holder Fig 5 14 Fuse Replacement b Replace two fuses with new ones then reattach...

Страница 169: ...nt 4 15 E E Line 2 2 3 46 ENT Enter 2 5 ERROR IN 2 2 Error Messages 4 33 ERROR OUT 2 2 ESC Escape 2 5 F FOCUS 5 12 Fastening between Component Units APPENDIX1 15 Flow Cell Check and Washing 4 24 Flow Cell Disassembly 4 26 Flow Cell Leakage 4 29 Fluorometry Notes on Handling Samples That Contain a High Concentration of the Compound of Interest 2 12 Fluorometry Advantages of Fluorometry 2 8 ...

Страница 170: ...rometry Temperature Dependency of Fluorescence Intensity 2 15 Frequency APPENDIX1 2 Fuse Replacement 5 20 G GLP 2 4 3 17 4 22 H Hg Lamp Replace 5 17 HORIZONTAL 5 12 HOLD 3 30 I INSERT 3 33 Installation APPENDIX1 1 Installation Conditions APPENDIX1 2 Installation Place APPENDIX1 5 Installation Unpacking APPENDIX1 1 L LAMP 4 1 LAMP OFF IN 2 2 LCD 3 1 LED 3 2 LOCK OFF 2 5 LOGBOOK 4 22 M MARKER 2 5 MA...

Страница 171: ...oltage APPENDIX1 2 PROCESSOR option 2 3 PROG 2 4 Power consumption 2 19 R Raman Scattering 2 11 Raman Signal Intensity Measuring 4 9 Raman Spectrum of Water Measuring 4 3 READY BUSY OUT 2 2 Rear Panel 2 2 RECORD 2 5 RECORDER option 2 3 RECORDER Background Spectrum 3 43 S Sample Pretreatment 3 65 Sensitivity Check of Sensitivity 4 2 SET UP 2 4 Specifications 2 17 SPECT 2 5 START 2 4 START IN 2 2 ST...

Страница 172: ...option 2 1 UTILTY Utility 2 4 V VERTICAL 5 12 W WL Wavelength 2 4 Wavelength Accuracy Checking by using Hg Lamp 4 2 4 21 Wavelength Calibration of Wavelength Accuracy 4 17 Wavelength Setting 3 6 Wiring Arrangement APPENDIX1 10 X Xenon Lamp Positional Adjustment 5 12 Xenon Lamp Cool Down 5 3 Xenon Lamp Replacement 5 3 ...

Страница 173: ...r to carry out installation at the time of delivery For ensuring safety and high accuracy in operation of the instrument installation of the instrument shall be carried out by qualified service personnel trained by the manufacturer or its authorized maintenance service agent Before installation however the user shall make adequate preparations for installing the instrument referring to this instru...

Страница 174: ...re for the total system The total system shown comprises Primaide organizer Primaide 1110 pump Primaide 1210 autosampler Primaide 1310 column oven and Primaide 1410 1430 1440 detector Check and prepare a power supply meeting the power supply requirements for your actual system configuration and OA equipment to be used in combination Power outlet A power outlet shall be available near the installat...

Страница 175: ...ilable use a grounded 3P table tap or a 3P to 2P adapter In this case be sure to provide a proper grounding connection For grounding connection use a screw having a diameter of M4 or higher and a turn count of 3 or more in threading and a wire having a thickness of 1 25 mm 2 Ground Terminal Shall have a grounding construction for a ground resistance of 100 or less a Ordinary Grounding Connection G...

Страница 176: ...ry Primaide1440 fluorescence detector Copper plate Connector Rear panel 300 mm 10 mm Ground electrode Underground Ground wire green Screw diameter M4 or larger Turn count 3 or more Wire thickness 1 25 mm b 1 With 2P Power Outlet Connection Ground terminal Power outlet having a ground terminal 3P to 2P adapter Primaide1440 fluorescence detector Connector Rear panel Ground wire green Screw diameter ...

Страница 177: ...d Primaide 1440 fluorescence detector stacked in two blocks are included Check and prepare an installation place satisfying the space requirements for your actual system and OA equipment to be used in combination 4 Installation Environment The following environmental conditions must be satisfied 1 Operating Ambient Temperature Within a range of 4 to 35 C And temperature variation should be minimum...

Страница 178: ...ic welder high frequency furnace pole transformer etc f Protect the instrument from excessive dust Dust would degrade the performance g Connect the instrument to a line that is free from abrupt voltage fluctuations Such would increase the detector noise h If a motor driven apparatus such as stirrer or vibrator not having a noise suppressing device is connected to the same power line as this instru...

Страница 179: ...m of the instrument Remove the transport screws with a phillips screwdriver Appended Fig 1 3 Removal of Transport Screws 2 Pull out the lamp house from the instrument see to in section 5 2 1 2 Transport screw 1 For the instrument fixing Transport screw 2 For the instrument fixing Transport screw 3 For lamp house fixing ...

Страница 180: ...tall the xenon lamp in the lamp house And mount it to the instrument see to in section 5 2 1 2 4 Adjust the position of the xenon lamp see section 5 2 1 3 NOTE After removing the transport screws please store them Xenon lamp Lamp house Anode nut Cathode nut ...

Страница 181: ... Isocratic System Low Pressure Gradient System stacked in one block Connect the power cables of the organizer and column oven to AC power source For the Primaide1110 pump connect the round connector cable from the organizer to the instrument power connector For the Primaide1210 autosampler connect the round connector cable from the organizer to the instrument power connector For the Primaide1410 1...

Страница 182: ...tion to AC power source For the Primaide 1110 pump Primaide 1210 autosampler plug the connector cable from the organizer into the power connector of each unit Table1 1 AC adapter option P N 8DD 0756 sold separately Please use Primaide1110 Pump Primaide 1210 Autosampler Primaide 1310 Column Oven To AC power supply Primaide 1440 Fluorescence Detector Primaide 1410 1430 UV DAD detector Primaide 1000 ...

Страница 183: ...ies it is required to connect the e Line cable between the instruments On the left rear of each instrument insert the e Line cable into the e Line connector Appended Fig 1 6 e Line Connector on the Rear of Instrument e Line connector Rear side of the instrument ...

Страница 184: ... Low Pressure Gradient System stacked in one block Primaide1110 Pump Primaide1210 Autosampler Primaide1310 Column Oven Primaide1410 1430 UV DAD Detector Primaide Organizer e Line cable e Line cable e Line cable Interface Control Board ...

Страница 185: ...o each unit e Line cable 80 cm P N 8DD 0579 attached to Fluorescence unit e Line cable 200 cm P N 8DD 0583 attached to Autosampler unit e Line cable e Line cable e Line cable Primaide 1110 Pump Primaide1210 Autosampler Primaide1310 Column Oven Interface Control Board Primaide1440 Fluorescence Detector Primaide1410 1430 UV DAD Detector Primaide Organizer ...

Страница 186: ...ng device to the inlet of the pump Connect the drain valve of the pump to port 1 of the injection valve of the autosampler Connect port 2 of the injection valve to the column inlet Connect the column outlet to the inlet tube of the detector cell For washing solution provide connection to port 1 of the syringe valve of the autosampler through the degassing device Drain tray 1 1 6 110 OO T 3 2 Inlet...

Страница 187: ...injection valve of the autosampler Connect port 2 of the injection valve to the column inlet Connect the column outlet to the inlet tube of the detector cell For detergent provide connection to the three way solenoid valve of the syringe valve of the autosampler through the degassing device 1 2 6 3 1 Column Mobile phase Wash fluid Degassing device Primaide 1440 Fluorescence detector Primaide 1310 ...

Страница 188: ...utosampler Connect port 2 of the injection valve to the column inlet Connect the column outlet to the inlet tube of the detector cell For washing solution provide connection to port 1 of the syringe valve of the autosampler through the degassing device 1 1 6 3 2 110 OO T Mixer Proportioning valve Drain Mobile Phase Column Injection valve Drain valve Detector cell Primaide1210 autosampler Primaide1...

Страница 189: ...mixer of the pump to port 1 of the injection valve of the autosampler Connect port 2 of the injection valve to the column inlet Connect the column outlet to the inlet tube of the detector cell For detergent provide connection to the three way solenoid valve of the syringe valve of the autosampler through the degassing device 1 2 6 3 1 Primaide 1440 Fluorescence detector Primaide 1310 Column oven D...

Страница 190: ... CLAMP SHEET Degrease the surface by ethanol etc Paste clamps Arrange eluent waste pipings as shown a stacked in one block Primaide Organizer Primaide1410 1430 UV DAD Detector Primaide 1310 Column Oven Primaide1210 Auto Sampler Primaide1110 Pump Clamp P N 8DD 1921 Clamp Sheet P N 8DD 1095 Eluent Waste Clamp P N 8DD 1920 Caution Do not tile to prevent backflow Caution Do not tile to prevent backflo...

Страница 191: ... two blocks Appended Fig 1 10 Tubing Diagram for Drain when stacking module units in two blocks Drain Primaide 1440 Fluorescence detector Primaide 1310 Column oven Primaide 1210 Autosampler Primaide Organizer Primaide 1110 Pump ...

Страница 192: ... tips over When installing the instrument provide a proper means for fastening between module units On the rear of each module unit a cabling cover is attached The adjacent models are fastened with each other by attaching the fastening plate between the cabling covers Appended Fig 1 11 Attachment of Cabling Cover Fastening plate Fastening screw ...

Страница 193: ...e contact signals that are input and output via 3 pin connector Their names and functions vary with the unit A name that indicates the function is given at each connector Example of output contact signal EVENT of pump Example of input contact signal LAMP OFF IN of detector 2 Contact Signals Entering the e Line Connector Each unit has two e Line connectors for connection at the rear of the main uni...

Страница 194: ...L 2000 Series Use the e DIO cable 8DD 0584 for connection To L 2000 series instrument To Primaide1000 series instrument e Line connector 3 pin connector BUSY ERROR STOP START e Line connector e DIO cable 8DD 0584 e DIO cable 8DD 0584 ...

Страница 195: ... e DIO cable 8DD 0585 for connection Connect the START L and G terminals of the e DIO cable 8DD 0585 to the EXTERNAL COM terminal of the Model D 2500 D 2500 START COM EXTERNAL H L BUSY H L G START BUSY e Line connector To Primaide1000 series instrument ...

Страница 196: ...tact signal Output Terminals These are contact signal input terminals for control using contact signals of an external unit The output circuit is shown in Fig 4 The rating of contacts used is 12 V 0 1 A Make sure the load connected does not exceed this rating Fig 1 e Line Cable Connection Fig 2 e Line Contact Circuit Configuration 5 V 320 IN Max 10 mA unit Max 0 1 A OUT 4 7 k ON 5 V OFF 0 V Max 12...

Страница 197: ...horting contacts of external unit Fig 3 Contact Signal Input Circuit Fig 4 Contact Signal Output Circuit 5 V 320 Primaide1000 series unit External unit Max 10 mA 0 1 A max 30 V max 1 2 3 Primaide1000 series unit External unit 1 2 3 ...

Страница 198: ...measurement Key Lock This protective function prevents an erroneous key input during operation of the instrument GLP The confidence function is used to check the status of instrument Fluorescence A kind of luminescence which is emitted from a sample when a stimulating excitation beam is given to it Emission Fluorescence intensity Unit of fluorescence Relavive intensity Emission Fluorescence wavele...

Страница 199: ...n arrangement a filter is used to remove or cut off the 2nd order scattered light Full scale Used to determine the magnitude of output to such an external device as data processor chromato integrator Processor The terminal for analog output to the data processor Flow Cell Contains a sample for measurement Photomultiplier Used to detect fluorescence emission Marker in The external contact signal ca...

Страница 200: ...lative time Indicates a cumulative total time of lamp turn on periods Ex Excitation wavelength Used for exciting a sample Recorder RECORDER Used for analog output to a recorder Logbook Means the information containing records of instrument operating conditions Utility Used to set up operating conditions of the instrument ...

Страница 201: ... tube Numbers Inside diameter 1 Isocratic System stacked in one block 1 1 6 110 OO T 3 2 Primaide1110 Pump Primaide1310 Column Oven Primaide1210 Autosampler Primaide1410 1430 UV DAD Detector Primaide Organizer Mobile Phase Drain Washing Solution Degassing device 1 5 TT Inlet tube Detector cell 0 33 TT Injection valve Syringe valve Drain valve 1 5TT 0 25 TT 0 25 ST 0 8 ST Drain OUT IN ...

Страница 202: ...locks 1 5 TT 1 5 TT 0 25ST 0 8ST 0 25TT 0 33TT 1 2 6 3 1 Column Mobile Phase Wash Solution Degassing device Primaide 1440 FL Detector Primaide 1310 Column Oven Primaide 1210 Autosampler Drain Detector cell Primaide Organizer Primaide 1110 Pump Drain ...

Страница 203: ... 2 110 OO T Primaide1110 Pump Primaide1310 Column Oven Primaide1210 Autosampler Primaide1410 1430 UV DAD Detector Degassing device Mobile Phase Organizer option Drain 1 5 TT Detector cell Inlet tube 0 25 TT Injection valve Syringe valve Drain valve 0 25 ST Column 0 8 ST 0 33 TT Drain OUT IN ...

Страница 204: ... two blocks Column 0 25ST 0 8ST 0 25TT 1 5 TT 1 5 TT 0 33TT 1 2 6 3 1 Primaide 1440 FL Detector Primaide 1310 Column Oven Drain Detector cell Primaide 1210 Autosampler Mobile Phase Wash Solution Primaide Organizer Primaide 1110 Pump Degassing device Mixer ...

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