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4
Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
SUBJECT
PAGE #
Electrofusion
23
Pre-Fusion Pulse AC
23
Fusion Pulse
23
Post-Fusion Pulse AC
23
Cell Handling After Electrofusion
24
Immediate Post-Fusion Care of Cells
24
Materials Needed for Post Electrofusion Culture
24
Cloning Medium 1
24
Cloning Medium 2
24
Complete Growth Medium
24
HAT Stock (50X)
24
HT Stock (50X)
24
Method, Culture of Cell and Clone Selection
25
Culture of Cells
25
Feeding Cells
25
Cloning Cells
25
Weaning Clones
25
Method of Analysis
25
Demonstration Experiment to Illustrate Turbulence
26
Optimization
26
Chamber Cleaning
26
Hybrimune Electrofusion Protocol Summary
27–28
Cell Electrofusion Tutorial
29–43
Applications
29
Hybridoma Production
29
Immunotherapy
30
Nuclear Transfer
30
Cell Fusion Methods
31
Electrofusion
32
Cell Alignment (Dielectrophoresis)
32–37
Waveforms for Electrofusion
37–43
Cell Alignment and Compression
38–39
AC Sine Wave Waveforms
39–41
Electrofusion Pulse
42
Post Fusion Pulse Sine Wave
42
Parameters Range of Hybrimune Waveform Generator
42
Published Waveform Protocols
43
Specifications
44–45
Maintenance
46
Fuse Replacement
46
Ordering Information
47
References
48
Appendices
49–51
Appendix A: Medarex Data
49–50
Appendix B: MEDCOAT 200 Summary Test Results
51
Table of Contents