![background image](http://html.mh-extra.com/html/harvard-bioscience/btx-hybrimune/btx-hybrimune_user-manual_601890034.webp)
34
Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
A cell radius of 7 µm is used in the model which is typical. The model shows, that within typical conductivities
of 80 to 200 µS/cm, the Clausius-Mossotti function is generally in the +0.90 to +0.95 range as long as waveform
frequencies are kept within the range of 0.2 to 2 MHz (red rectangle). The Hybrimune System including
waveform and medium was designed to operate in this range. It should be noted that the nominal conductivity
of Cytofusion™ medium is 80 µS/cm. After adding properly washed cells the suspension will generally have a
conductivity of 100 to 200 µS/cm.
Clausius-Mossotti Function
cell radius 7 um
Frequency MHz
1 . 0 0 0
0 . 8 0 0
0 . 6 0 0
0 . 4 0 0
0 . 2 0 0
0 . 0 0 0
- 0 . 2 0 0
- 0 . 4 0 0
- 0 . 6 0 0
- 0 . 8 0 0
- 1 . 0 0 0
0 . 0 0 1
0 . 0 1
0 . 1
1
1 0
1 0 0
1 0 0 0
80 µS/cm
200 µS/cm
K
A computer model of this formula is presented in Figure 13.
K IS NOT UNDER USER CONTROL
Figure 13: Clausius-Mossotti Function
Cell Electrofusion Tutorial