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Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
Chamber:
This box displays whether or not the
chamber cable is connected and ready to
receive pulses.
OK:
The chamber cable is connected; the
safety interlock is satisfied.
Open:
The chamber cable is not connected;
the safety interlock is not satisfied.
High Voltage:
Displays the state of the internal
high voltage power supply (HVPS).
On:
The power supply is on and the reservoir
capacitor is charging.
Off:
The power supply is off.
Status:
This box shows the current functional status.
It displays:
Ready:
The system is ready to accept
instructions to begin a protocol.
Download:
The computer is loading
protocol to the internal microprocessor.
Charging:
The system is charging the
reservoir capacitor to the set voltage.
Pulsing:
The protocol is being delivered to
the chamber.
Monitors
Power Supply Voltmeter:
Displays the voltage
of the reservoir capacitor, not the pulse
amplitude. This display is operational at all
times except when pulses are being delivered.
During charging:
Displays the voltage,
updated every second or so.
After charging:
Displays the final voltage on
the reservoir capacitor.
W. Generator at reset:
Displays the system
rest voltage.
Last Protocol Log Window
The window at the bottom center of the screen is the Last Protocol
Log window. The window displays the last protocol executed.
Control Panel Area
The Electroporation Mode Control Panel Area in the center of the
screen is where the pulse-protocol parameters are set, edited, and
reviewed. The following is a description of each of the sections,
and input/display boxes found in the Control Panel.
• Group ID:
Displays the group number whose
characteristics are shown in the rest of the
Control Panel Area. The Group ID is also shown
highlighted in the Group List.
• Pre-SINE
Beg. Voltage:
Beginning voltage
Duration:
Duration in seconds
Frequency:
Frequency in Megahertz
End Voltage:
End voltage
Ramp k:
Non-linear ramp constant
• Pulse
• Amplitude:
Voltage of the electroporation fusion pulse
• Duration:
Length of the pulse
• Pulses:
Number of pulses to deliver
• Interval:
Delay between the start of each pulse
• Post-Sine
Beg. Voltage:
Beginning voltage
Duration:
Duration in seconds
Frequency:
Frequency in Megahertz
End Voltage:
End voltage
Ramp k:
Non-linear ramp constant
Hybrimune
™
System Components & Set-Up