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18
Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
This section describes various methods to program waveform
protocols. As stated previously there generally are three waveform
components:
•
Pre-fusion Pulse AC
• Fusion Pulse
• Post-fusion Pulse AC
The waveform generator user interface uses the concept of Groups.
A group is defined as a set of AC + Pulse +AC waveforms in which
the parameters of the first AC, the pulse and the last AC do not
change. The one exception is the change in AC amplitude. The
AC amplitude can be varies by changing the Ramp k parameter. A
group does not have to consist of all thee components, only one of
the three is required for a valid group.
•
To inhibit or cause an AC waveform not to appear select
zero for the duration
• To inhibit or cause the pulse not to appear select zero for
the number of pulses
The software automatically concatenates all of the Groups in the
proper order to be executed in series.
The programming of the recommended protocol will be
demonstrated.
Programming the Protocol
This protocol requires two groups because the pre fusion pulse AC
has two parts:
Note that setting pulse number equal to zero inhibits pulses and
setting AC duration to zero inhibits AC.
To program:
• Enter the values for Group 1 in the user interface
• Click Add
• Enter the values for Group 2 in the user interface
• Click Add
• Save as BTX
This can also be programmed as four groups:
• AC1
• AC2
• Pulse
• AC
GROUP 1
Pre-sine
Start
40 volts peak
Stop
40 volts peak
Function
Linear (Ramp k =1)
Duration
15 seconds
Frequency
0.8 Mhz
Pulse
Amplitude
set at Group 2 value
Width
set at Group 2 value
Interval
set at Group 2 value
Number
0
Post-sine
Start
set at Group 2 value
Stop
set at Group 2 value
Function
set at Group 2 value
Duration
0
Frequency
set at Group 2 value
GROUP 2
Pre-sine
Start
75 volts peak
Stop
75 volts peak
Function
Linear (Ramp k =1)
Duration
20 seconds
Frequency
0.8 Mhz
Pulse
Amplitude
800 volts
Width
40 µs (entered as 0.040
milliseconds)
Interval
0.125 seconds
Number
1
Post-sine
Start
60 volts peak
Stop
5 volts peak
Function
Linear (Ramp k=1)
Duration
30 seconds
Frequency
0.8 MHz
Programming Waveform Protocols
Hybrimune
™
System Components & Set-Up