3
Hybrimune Hybridoma Production System
Publication 015-1010191 Rev 4.0 • www.btxonline.com
SUBJECT
PAGE #
Warranty Information
6
Safety Information
7
Hybrimune™ System Components and Set-Up
8–18
Introduction
8
The Hybrimune™ Waveform Generator
8
The Large-Volume Fusion Chamber
9
Cytofusion® Medium
9
Setting up the Hybrimune™ System
10
Waveform Generator Front Panel Display
10
System Status Group
10
Process Status Group
11
Waveform Generator Interface Software Installation
11
Set-up using a USB-to-Serial Converter
11
System Test
12–13
Elementary Verification Tests
14
The Waveform Generator Interface Software
14
The Tool Bar
15
Tools Area
15
Status Area
15–16
Last Protocol Log Window
16
Control Panel Area
16–17
Running a Protocol
17
File Management
17
Programming Waveform Protocols
18
Programming the Protocol
18
Producing Fusion Products Using Hybrimune
19–26
Cell Preparation Before Electrofusion Day
19
Myeloma Cells
19
Available Myelomas or Heteromyelomas
20
Human
20
Mouse
20
Rat
20
Rabbit
20
B-Cells
20
Immunization
20
Screening
20
Harvesting Spleen Cells
21
In Vitro Activation
21
Cell Preparation on Electrofusion Day
21
Medium
21–22
Chamber Cleaning
22
Washes and Other Preparatory Needs
22
Cell Preparation Process
22
Table of Contents