GE Amersham ECl Gel Box, Руководство пользователя

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Sample loading
To load the samples, follow the instructions:
Action Step
Prepare the samples by adding sample and 2× sample buffer in
a 1:1 mixture. 1
Example: for 10 μl sample, add 10 μl 2× sample buffer.
Note:
For native conditions: use a sample buffer without SDS and DTT.
Heat the samples at 95°C for 5 minutes. 2
Spin down the samples quickly in a microcentrifuge and load the
samples directly into the wells in the gel.
Note:
A maximum of 0.5 μg/band per well of sample can be loaded.
Overloading may cause smearing and distortion.
3
Note:
To ensure uniform mobility, load an equal volume of 1× SDS
sample buffer into any unused well.
Place the safety lid on top of Amersham ECL Gel Box. 4
Running conditions
Run the gel according to the recommendations below.
Value Parameter
160 V Voltage
Start: approx. 45 mA
End: 23 to 33 mA Expected current
42 Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC
5 Protocols
5.1 Electrophoresis protocol