GE Amersham ECl Gel Box, Руководство пользователя

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Gel specifications
Value Parameter
Acrylamide/bis-acrylamide Gel matrix
%C=2.6% (w/w) Gel concentration
%T=Total concentration (w/v) of acry
bisacrylamide is given as percentage in the
respective Amersham ECL Gel product name.
1.4 mm Gel thickness
7.5 cm x 8.0 cm (W x H) Gel size
2, 10, and 15 well gels are available. Sample well
configuration
100 μl
35 μl
20 μl
Sample well volume
2 wells
10 wells
15 wells
4% Stacking gel
Tris-HCl Buffer system in gel
•
25 mM Tris, 192 mM glycine, 0.1% SDS,
pH 8.3
•
25 mM Tris, 192 mM glycine, pH 8.3
Running buffer:
•
Denatured
conditions
•
Native conditions
e.g. Tris-HCl-SDS or any other buffer suitable
for the application Sample buffer
12 months from date of manufacture Shelf life
32 Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC
3 System description
3.2 Amersham ECL Gel