6
Introduction
The “DTlite” Detecting Thermocycler (hereafter – the “instrument” or “device”) is intended
to conduct qualitative and quantitative PCR analysis dropping out electrophoresis stage of
PCR products in agarose gel when using PCR kits based on the fluorescent detection princi-
ple. Measurement of the quantity of accumulated product of PCR amplification takes place
right in the course of reaction temperature cycles (real-time PCR). The qualitative analysis is
based on evaluation of kinetics of PCR visible part, carried out using relevant mathematical
tool.
The use of fluorescent detection of PCR products has a list of substantial advantages:
high detection specificity (if oligonucleotide probes are applied, which allow detecting
only of certain amplicons);
high efficiency;
reduced assay time;
carrying out detection in a closed tube, which practically eliminates contamination of
further experiments;
availability of quantitative assessment of initial DNA-matrix;
registering and accounting of the data in electronic format.
Fig.1 Instrument general view
