29
Caution!
To measure tubes height, use at least 16 tubes or 2 strips putting them evenly
throughout thermal plate.
4.3 Creation of plate protocol and amplification program
After switching on the instrument create or edit of a plate protocol and amplification program
using RealTime_PCR software.
4.3.1. Filling in of plate protocol
The window for plate protocol filling in (Fig.23) is displayed immediately after starting
RealTime_PCR and comprises: data input table, control buttons for plate protocol filling in
and a field of graphic representation of tubes arrangement in the thermal block.
Fill-in plate protocol in one of the following ways:
by using
Test
procedure with saved standard assay parameters (recommended for
standard assays in clinical laboratories);
by using plate protocol templates saved before.
Note!
Do not fill in the plate protocol without using Test procedure, as window Analysis
Parameters won’t be active, and the optical calibration coefficients of the instrument
will not be used
.
In the course of the filling in the plate protocol, enter the following data:
identification code (description) of each tube;
type of each tube (sample, standard or c/- );;
the number of replicas of each sample;
concentration of calibration samples (in a quantitative assay);
fluorophore is used (fluorescent label) and its purpose (specific target, IC or not de-
tected);
arrangement of tubes in the thermocycler block;
Plate protocol No.;
operator’s name.
The procedure of creating (editing) the plate protocol is described in details in the second part
of the operation manual
Software guidance
.
