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Caution, hot surface!
During the instrument operation, the matrix of thermal block and hot
lid may get hot up to 100 and 105 ºC respectively. When execution of the PCR RUN (operat-
ing mode) the thermal block matrix and the hot lid are not available for operator. Upon com-
pletion of the PCR RUN operator is not allowed to remove the tubes from the thermo block
until the instrument enters in storage mode (low temperature mode) with the following mes-
sage:
4.5 Startup of the amplification program
Enter necessary notes such as the particularities and characteristics of the upcoming run and
the PCR mix volume in tubes;
Be sure that the instrument is on and properly connected to the computer, which is confirmed
by yellow
or green
indicator in the software status line.
Start the amplification program by clicking
.
When running the amplification program, select a file name, data format and folder to save
future results of the upcoming RUN.
Note!
If
Run
is started while the instrument is in the process of warming up (yellow indicator
in the software status line), blinking message
Warming
will be appeared
in
Temperature Profile
window:
The same message will appear at the instrument’s display as well. When warming up is com-
pleted, the instrument will automatically proceed to the RUN execution mode.
In case of necessity of instrument’s autonomous functioning after the RUN completion and its
automatic turning to sleep mode perform the following:
1. In
Running
window activate the option - turn off device after the RUN
2. Close the Real Time PCR software by clicking
in the upper right corner of its win-
dow.
3. When RUN is completed the instrument will switch to the sleep mode, what is confirmed
by blinking diode on the front panel of the instrument.
Note!
The instrument will proceed to the sleep mode only if “
Turn off device after the run”
option is activated and the executed amplification program finishes as
Holding
mode.
