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4.6 Amplification program running
Running of the amplification program is controlled via RealTime_PCR software.
The following options could be done during its execution:
pause running;
stop running;
add any number of cycles in a current block or skip execution of the remaining cycles
and proceed to the execution of the next temperature block;
edit the log of optical measurement;
to check optical measurement data of the selected tubes in the form of 3D histogram in
the
Distribution of optical measurements over the plate
window by clicking
(Fig.27).
Fig.27
Distribution of optical measurements over the plate
window
If option
“Turn off device after the run”
was not activated, the instrument passes to the
Holding
mode after completion of the RUN (Fig. 28).
In this case, a blinking message Holding… and a information about storage temperature will
appear on the chart.
Fig.28 Message about the RUN completion
The same message will appear on the instrument display as well.
When the temperature reaches the value close to holding temperature information message
appears on the screen notifying that the RUN has completed and instrument passes to the
holding mode (Fig.29).