ChemoMetec NucleoCounter, Руководство пользователя

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2 Introducing the NucleoCounter
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The measurement chamber is illuminated by green excitation
light from light source. The green light is passed through an
excitation filter before it reaches the measurement chamber.
The excitation filter allows only green light of the
appropriate wavelengths for the excitation of PI to pass.
The green light will excite the PI bound to DNA and in return
PI emits red fluorescence light. Some of the green light will
pass through the measurement chamber together with the red
fluorescence light. This green light is removed by an emission
filter, which only allows the red light to pass through. Using
an optical lens system the red light is finally focused on the
CCD chip of the camera.
A fluorescent image of the content of the measurement chamber
is thus achieved. Example of such an image is shown in Figure
4. Each spot in the image represents a cell containing DNA
stained with PI.
Figure 4 . Fluorescent image (from NucleoView PC software) of
cell DNA contained in the measurement chamber of a
NucleoCassette
2.1.2 Built-in image analysis
The NucleoCounter is equipped with advanced software for image
analysis. This integral software analyzes the recorded image
and counts the number of nuclei in the image. As the volume of
the cassette measurement chamber and the dilution of the lyzate
is known, the cell density in the initial sample can be
calculated. The concentration of cells in the initial sample is
presented in the NucleoCounter display. If the optional
software (NucleoView) is used, the results and the recorded
fluorescent image can be viewed.