ChemoMetec NucleoCounter, Руководство пользователя

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Introduction and intended use
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Introduction and intended use
The NucleoCounter
®
is a novel instrument designed specially to
count mammalian cells. The instrument is a part of the
NucleoCounter system, which also comprises the NucleoCassette™,
Lysis buffer (Reagent A100) and Stabilizing buffer (Reagent B).
The NucleoCounter system allows reliable, fast and objective
cell counting to be carried out based on the method of
fluorescence microscopy. It also makes it possible for the user
to stain and count mammalian cells without handling hazardous
dyes.
The NucleoCounter system has been developed to count mammalian
cells in suspension. The mammalian cells could be cultured in
T-flasks, bioreactors or on the surface of micro carriers.
The NucleoCounter is developed as a stand-alone instrument but
optionally it can be connected to a computer via an USB-
interface. When connected to a computer the NucleoView
TM
software offers various features such as documentation of the
obtained results. Read more about the software in the
NucleoView
TM
User´s Guide.
The NucleoCounter type at ChemoMetec A/S is 900-0004.
This user´s guide is valid for NucleoCounter software
version 4.XX
The NucleoCounter system enables the user to obtain cell counts
and viability determinations on cell suspensions from a wide
range of cultured mammalian cell lines.
The cell counts and viability determination rest on the ability
of the fluorescent dye to differentiate between viable and non-
viable cells by means of different permeability of the plasma
membranes. Thereby the concentration of viable and non-viable
cells in suspension may be determined using the NucleoCounter
system.