2 Introducing the NucleoCounter
5
2 Introducing the NucleoCounter
The NucleoCounter method provides a fast alternative to the
manual techniques, yielding objective and reliable results.
Furthermore the NucleoCounter technology provides improved
safety to the user compared to other comparable methods.
The NucleoCounter system is comprised of the NucleoCassette for
sample handling, the NucleoCounter instrument for particle
analysis, a Lysis buffer for cell lysis (please refer to
appropriate Application Notes provided by ChemoMetec concerning
lysing of cells), and a Stabilizing buffer for optimizing the
pH of the lysed cell suspension (please refer to appropriate
Application Notes provided by ChemoMetec concerning stabilizing
of the lysed cell suspension). Basics of the NucleoCounter
system is explained on the next few pages.
The NucleoCounter system identifies and counts individual cell
containing stained DNA. The sample pre-treatment is intended to
allow the effective staining of cellular DNA but it will also
aid the dissolving of cell aggregates. Depending on cell
species and sample properties some additional effort might be
needed to dissolve cell aggregates (please refer to appropriate
Application Notes provided by ChemoMetec).
2.1
The NucleoCounter instrument
The NucleoCounter is shown in the figure below.
Figure 2.
NucleoCounter instrument
Lid
Control Buttons
Keypad
Display
Weight 3 kg
Height
26 cm
Width
38 cm
Depth
22 cm
Содержание NucleoCounter
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