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Büchi Labortechnik AG

Appendix | 10

Operation Manual Pure Chromatography Instruments

97/102

10.3

Finding out separation conditions with the navigator

10.3.1 Open the navigator

Navigation path

➔

Gradient panel

Navigate to the

Gradient

panel according the navigation path.

Tap the button

[Navigator]

The display shows the dialog

Navigator

10.3.2 TLC Silica

The TLC-Silica tool uses TLC separation data to provide a recommended gradient for
silica separations of 2 or 3 components.
Specification of the samples needed:

Two different solvent concentrations

Two separations

Silica TLC plates

Precondition:

The display shows the dialog

Navigator

. See Chapter 10.3.1 "Open the

navigator", page 97

Select the

TLC- Silica

tab.

Select the radio button for two or three components.

Select the weaker solvent at drop-down list A.

Select the stronger solvent at drop-down list B.

Enter the solvent B concentrations used in the TLC separations.

All manuals and user guides at all-guides.com

Содержание Pure C-810

Страница 1: ...Pure Chromatography Instruments Operation Manual 11593990 E en All manuals and user guides at all guides com ...

Страница 2: ...ake changes to the manual as deemed necessary in the light of experience especially with respect to structure illustrations and technical details This manual is copyrighted Information from it may neither be reproduced distributed or used for competitive purposes nor made available to third parties The manufacture of any component with the aid of this manual without prior written agreement is also...

Страница 3: ...due to connections with other devices or network 11 2 5 3 Damage to the internal memory due to incorrect shutting down of the instrument 12 2 6 Personal protective equipment 12 2 7 Modifications 12 3 Product description 13 3 1 Description of function 13 3 2 Configuration 14 3 2 1 Front view Pure C 810 C 815 14 3 2 2 Front view Pure C 830 C 835 C 850 15 3 2 3 Rear view 15 3 2 4 Connections on gradi...

Страница 4: ...level sensor 26 5 7 Assigning solvent to solvent lines 26 5 8 Calibrating the solvent level sensor 27 5 9 Installing the exhaust ELSD only 28 5 10 Assembling the waste bottle 28 5 11 Installing the waste level sensor 28 5 12 Calibrating the waste level sensor 29 5 13 Assembling the dry air supply option 29 5 14 Assembling the gas connection option 30 5 15 Assembling the solvent bottle platform opt...

Страница 5: ... a separation 54 6 4 1 Introducing a sample into the prep system 54 6 4 2 Injecting a sample into the flash system 55 6 4 3 Installing and removing a cartridge 57 6 4 4 Installing and removing a column 58 6 4 5 Inserting the fraction collection tray 60 6 4 6 Selecting an existing method 61 6 4 7 Selecting values on the dialog box Sample Loading 61 6 5 Performing a Flash separation using a method 6...

Страница 6: ...DF to USB 72 7 Cleaning and servicing 73 7 1 Regular maintenance work 73 7 2 Carrying out a data backup 74 7 3 Calibrating the display 74 7 4 Cleaning the nebulizer 75 7 5 Cleaning the check valve 76 7 6 Cleaning the instrument 77 7 7 Cleaning the solid loader 78 7 8 Removing solvent from an used cartridge 78 8 Help with faults 79 8 1 Faults possible causes and solutions general 79 8 2 Faults poss...

Страница 7: ...ies 93 10 1 2 Sample introduction accessories 94 10 1 3 Spare parts 95 10 1 4 Tubes 95 10 1 5 Tools and adapter kits 95 10 1 6 Wear parts 96 10 2 Folder locations 96 10 3 Finding out separation conditions with the navigator 97 10 3 1 Open the navigator 97 10 3 2 TLC Silica 97 10 3 3 LC C18 98 10 3 4 LC Transfer 99 All manuals and user guides at all guides com ...

Страница 8: ...ices warn you of dangers that can occur when handling the device There are four danger levels each identifiable by the signal word used Signal word Meaning DANGER Indicates a danger with a high level of risk which could result in death or serious injury if not prevented WARNING Indicates a danger with a medium level of risk which could result in death or serious injury if not prevented CAUTION Ind...

Страница 9: ...p like this Dialog Dialogs are marked up like this Button Buttons are marked up like this Field names Field names are marked up like this Menu Menu item Menus or menu items are marked up like this Status Status is marked up like this Signal Signals are marked up like this 1 3 Trademarks Product names and registered or unregistered trademarks that are used in this document are used only for identif...

Страница 10: ...ed to greater dangers The device may only be operated by suitably qualified laboratory staff These operating instructions are aimed at the following target groups Users Users are persons that meet the following criteria They have been instructed in the use of the device They are familiar with the contents of these operating instructions and the applicable safety regulations and apply them They are...

Страница 11: ... if the device is used incorrectly Appropriate warnings in this manual serve to alert the user to these residual dangers 2 5 1 Faults during operation If a device is damaged sharp edges moving parts or exposed electrical wires can cause injuries u Regularly check device for visible damage u If faults occur switch off the device immediately unplug the power cord and inform the operator u Do not con...

Страница 12: ...ear appropriate personal protective equipment such as safety goggles protective clothing and gloves u Make sure that the personal protective equipment meets the requirements of the safety data sheets for all chemicals used 2 7 Modifications Unauthorized modifications can effect safety and lead to accidents u Use only genuine BUCHI accessories spare parts and consumables u Carry out technical chang...

Страница 13: ... to separate gram size samples in short period Prep HPLC has the ability to separate complex samples at high resolution Pure instruments allow 4 different solvents Injection of liquid or solid sample Separation on a cartridge or column Identifying the compounds by UV and or ELS detection Collecting the desired fractions Pure instrument schematic Gradient valve UV detector Fraction collection Solve...

Страница 14: ... Front view Pure C 810 C 815 5 10 9 8 1 2 3 4 6 7 Fig 2 Front view Pure C 810 C 815 1 Control panel 2 RFID reader 3 On Off switch 4 USB Port 5 Injection port 6 Cartridge holder 7 Flash pump 8 ELSD flow split valve C 815 only 9 ELSD nebulizer C 815 only 10 Fraction collection bay All manuals and user guides at all guides com ...

Страница 15: ...iew Pure C 830 C 835 C 850 1 Control panel 2 RFID reader C 850 only 3 On Off switch 4 USB Port 5 Column holder 6 Prep HPLC pump 7 ELSD flow split valve C 835 C 850 only 8 ELSD nebulizer C 835 C 850 only 9 Prep sample injection valve 10 Fraction collection bay 3 2 3 Rear view NOTE All electrical connections are not limited energy All manuals and user guides at all guides com ...

Страница 16: ...iew 1 Ventilation slot 2 Gradient valve See Chapter 3 2 4 Connections on gradient valve page 17 3 Exhaust used for instruments with ELSD only 4 Signal connection for external air supply 5 USB ports 6 Power supply connection 7 Fuse 8 On Off master switch 9 Pressurized air inlet 10 LAN port All manuals and user guides at all guides com ...

Страница 17: ...r side 1 Waste line 2 Solvent line 4 3 Solvent line 3 4 Waste level sensor 5 Solvent line 2 6 Solvent line 1 7 Solvent level sensor line 1 8 Solvent level sensor line 2 9 Solvent level sensor line 3 10 Solvent level sensor line 4 3 3 Type plate The type plate identifies the instrument The type plate is located at the rear of the instrument All manuals and user guides at all guides com ...

Страница 18: ...y NOTE The scope of delivery depends of the configuration of the purchase order Accessories are delivered as per the purchase order order confirmation and delivery note 3 5 Technical data 3 5 1 Pure Chromatography Instruments Pure C 810 Pure C 815 Pure C 830 Pure C 835 Pure C 850 Dimension W x D x H 365 x 570 x 680 mm 365 x 570 x 680 mm 365 x 570 x 680 mm 365 x 570 x 680 mm 365 x 570 x 680 mm Weig...

Страница 19: ...lector bay closed closed closed closed closed Lighted fraction col lector bay Yes Yes Yes Yes Yes Solvent level sensor Yes Yes Yes Yes Yes Waste level sensor Yes Yes Yes Yes Yes Vapor sensor Yes Yes Yes Yes Yes Pump Flash Mode Pure C 810 Pure C 815 Pure C 830 Pure C 835 Pure C 850 Gradient binary binary binary Pressure range 0 50 bar 0 50 bar 0 50 bar Flow rate 0 250 mL min 0 250 mL min 0 250 mL m...

Страница 20: ...m 200 800 nm 200 800 nm Light sources Halogen Deuterium Halogen Deuterium Halogen Deuterium Halogen Deuterium Halogen Deuterium Detector DAD DAD DAD DAD DAD DAD scan entire range entire range entire range entire range entire range ELSD Detector Pure C 810 Pure C 815 Pure C 830 Pure C 835 Pure C 850 Light output laser 0 3 mW 1 mW 1 mW Pressure carrier air 0 8 bar 0 8 bar 0 8 bar Pressure spray air ...

Страница 21: ...Component Materials of construction Housing Powder coated steel 1 4301 Fraction collection bay PMMA PET Pump head PEEK Pump cover PP Pump excenter housing Aluminum Tubings FEP Fitting POM Valve screw fitting POM Ferrule ETFE Cone ring POM Radial seal PTFE Pistons Ceramic All manuals and user guides at all guides com ...

Страница 22: ... transit u After transportation check the device for damage u Damage that has occurred in transit should be reported to the carrier u Keep packing for future transportation 4 2 Storage u Make sure that the ambient conditions are complied with see Chapter 3 5 Technical data page 18 u Make sure a clean solvent like ethanol or isopropanol is in the pump u Wherever possible store the device in its ori...

Страница 23: ...nsportation The possible consequences are crushing injuries cuts and breakages u The instrument should be transported by two persons at the same time u Lift the instrument at the points indicated u Lift the instrument this requires two persons each lifting at one of the points indicated on the bottom of the instrument All manuals and user guides at all guides com ...

Страница 24: ... 200 mm Do not expose the instrument to any external thermal loads such as direct solar radiation Do not expose the instrument to increased electromagnetic emissions Electromagnetic fields in the frequency range between 200 to 300 MHz can cause the instrument to operate incorrectly Make sure that the installation site meets the requirements of the safety data sheets for all solvents and samples us...

Страница 25: ...ower cables The power supply cables supplied with the product by BUCHI precisely match the requirements of the device If other power cables that do not meet those requirements are used the device may be damaged and or its performance diminished u Use only the power supply cables supplied with the product or ordered separately from BUCHI u If using any other power supply cables make sure that they ...

Страница 26: ... solvent level sensor See Chapter 5 6 Installing the solvent level sensor page 26 5 6 Installing the solvent level sensor Precondition R Make sure that the instrument is not connected to the power supply u Attach all solvent level sensors to the instrument Connections see Chapter 3 2 4 Connections on gradient valve page 17 u Calibrate the solvent level sensors See Chapter 5 8 Calibrating the solve...

Страница 27: ...r Line 1 is assigned ð The drop down list closes u Repeat the solvent selection for each line u Activate the checkbox next to the line you wish to prime u Tap the button Auto Prime u Wait till priming finished u Tap the button Close ð All lines are assigned with solvents ð The dialog box closes 5 8 Calibrating the solvent level sensor Navigation path Tools Calibration and Defaults Precondition R T...

Страница 28: ...es u Make sure that there is a decline between the connection on the instrument and the waste bottle u Put the waste line into the waste bottle 5 11 Installing the waste level sensor Precondition R Make sure that the instrument is not connected to the power supply u Attach the waste level sensor to the instrument Connections see Chapter 3 2 4 Connections on gradient valve page 17 u Calibrate the w...

Страница 29: ...quid waste u Navigate to the Calibration dialog according to the navigation path ð The display shows the dialog box Calibration u Tap Zero for Waste u Tap the button Close ð The dialog box closes 5 13 Assembling the dry air supply option u Connect the signal cable from the air supply to the instrument u Connect the gas connection See Chapter 5 14 Assembling the gas connection option page 30 All ma...

Страница 30: ...s with the specifications See Chapter 3 5 Technical data page 18 u Cut 50 mm from the gas tubing u Attach the cut tubing to the air filter u Secure the tubing in place u Attach the tubing from gas supply to the other side of the air filter u Secure the tubing in place u Connect the tubing from the gas supply to the instrument All manuals and user guides at all guides com ...

Страница 31: ... bottle on top of the instrument can cause property damages u Do not place the waste bottle an the solvent bottle platform u Turn the solvent bottle platform upside down u Attach the drain line adapter to the solvent bottle platform u Attach the drain line to the drain line adapter u Attach the solvent bottle platform to the instrument with screws All manuals and user guides at all guides com ...

Страница 32: ...nik AG 32 102 Operation Manual Pure Chromatography Instruments 5 16 Assembling the sample loop Prep instruments only u Attach the sample loop to the instrument at the position indicated All manuals and user guides at all guides com ...

Страница 33: ...anel Shows the properties and default settings of the installed column cartridge See Chapter 6 1 4 Conditions panel page 37 3 Wavelength panel Shows available wavelengths and scan options 4 Collection panel Shows collection options See Chapter 6 1 5 Collection panel page 38 5 Gradient panel Shows chromatograms and gradient ta ble See Chapter 6 1 6 Gradient panel page 39 6 Run panel Shows the opera...

Страница 34: ... 1 9 Detector selection panel page 41 9 Slope detection panel Shows selectable slope detection options See Chapter 6 1 10 Slope detection panel page 42 6 1 2 Enter value Enter numbers u Tab on an entry field ð The display shows a dialog box with a numeric input box u Enter the value u Tap the button OK ð The value is saved ð The dialog box closes Enter names u Tab on an entry field ð The display s...

Страница 35: ... 52 Open Run Load a completed run Print Run Report See Chapter 6 10 1 Printing a run re port page 71 Print PDF to USB See Chapter 6 10 3 Sending PDF to USB page 72 Exit Exit the Pure software to Windows sys tem software Shut down The instrument shuts down Mode Flash Select the Flash mode See Chapter 6 5 2 Selecting the flash mode Pure C 850 only page 62 Preparative Select the Prep mode See Chapter...

Страница 36: ...g the solvent level sensor page 27 Calibrate the waste level sensor See Chapter 5 12 Calibrating the waste level sensor page 29 Set alarms Manual control Maintenance actions See Chapter 7 6 Cleaning the instru ment page 77 See Chapter 7 7 Cleaning the solid loader page 78 See Chapter 7 8 Removing solvent from an used cartridge page 78 See Chapter 8 8 Resetting the Fraction collector arm page 89 NP...

Страница 37: ... of the column in stalled in the instrument Flow Rate Enter value Edit the default flow rate Duration Units Choice of the duration type The following types are available minutes column volumes Equilibration Enter value Indicates the period of time or num ber of column volumes that the mo bile phase flows through the column before the sample is injected Run Length Enter value According to the curre...

Страница 38: ... the trays The trays are detected by the auto recognition The estimated number of vials required for the separation is displayed below the solvent usage list in the lower left corner of the Setup window The fraction collection area contains the following settings Action Option Explanation Fraction collection options Select value The following options are available Collect Peaks Collect All Collect...

Страница 39: ...or Finding out Flash sepa ration conditions See Chapter 10 3 Finding out sepa ration conditions with the navigator page 97 UV scan details View Shows the following charts 3D UV Time Wavelength 2D Time Wavelength Absorption maxima All scan maxima Zoom Function Zoom the graph Options Select values Graph options Edit Select between the op tions The graph is in edit mode See Chap ter Editing the gradi...

Страница 40: ...s over the set thresh old 6 1 7 Run panel The run panel shows available functions according to the current operation Symbol Description Meaning Start Is used to start the run or re start the actual operation if the system has been paused Stop Is used to terminate the operation of the system Pause Is used to stop the actual operation If the system is paused due to an er ror this button will change ...

Страница 41: ...panel Select solvents for a separation See Chapter 6 2 3 Selecting a solvent page 45 6 1 9 Detector selection panel Action Option Explanation ELSD Enable Disable Select value Action enabled Select between Low High Enter values for threshold Action disabled No selection All manuals and user guides at all guides com ...

Страница 42: ...above the fraction collector starts to collect fractions 6 1 10 Slope detection panel Action Option Explanation Slope detection Enable Disable Action enabled Fraction collection triggered by slope Action disabled No detection 6 2 Editing a method 6 2 1 Selecting a cartridge Flash mode NOTE The automatic reading of the cartridge information can only be done with specific BUCHI RFID tagged cartridge...

Страница 43: ...ormation about the selected col umn Navigation path Conditions panel u Navigate to the Conditions selection panel according the navigation path u Tap the input box next to Column ð The display shows a menu with selectable cartridges u Select the cartridges you wish to use All manuals and user guides at all guides com ...

Страница 44: ...ap the input box next to Column ð The display shows a dialog with an alphanumeric input box u Enter a name for the column u Tap the button OK u Tap the input box next to Flow rate ð The display shows a dialog box with a numeric input box u Enter a value for the flow rate u Tap the button OK u Tap the input ox next to Max Pressure ð The display shows a dialog box with a numeric input box u Enter th...

Страница 45: ...u Tap the drop down list next to A ð The display shows a drop down list with the assigned solvents u Tap the required solvent ð The solvent is selected ð The drop down list closes u Select more solvents for the mobile phase according to your needs 6 2 4 Editing the gradient The composition of the mobile phase as a function of time can be indicated by entering the gradient Four solvent lines can be...

Страница 46: ...e in col umn 2nd is reached Solvents Select value Select solvent line combinations 2nd Enter value Enter the percentage rate for the second solvent Navigation path Gradient panel u Navigate to the Gradient panel according the navigation path u Tap the button Table ð The display shows the dialog Gradient Table u Tap the cell for Min ð The display shows a dialog box with a numeric input box All manu...

Страница 47: ...st with selectable actions u Select if the line should be added above or below the selected line ð A line is added Deleting lines from the Gradient table u Tap the number field e g 1 ð The display shows a drop down list with selectable actions u Select delete ð The line is deleted Editing the gradient in graphic mode Navigation path Gradient panel u Navigate to the Gradient panel according the nav...

Страница 48: ...cking on the AB box to reveal a drop down list 6 2 5 Editing detector selection Navigation path Detector selection panel u Navigate to the Detector selection panel according the navigation path u Activate checkbox for the detector you wish to use ð The detector is activated 6 2 6 Editing the wavelength for the UV detector All manuals and user guides at all guides com ...

Страница 49: ...ctor and does not col lect fractions Navigation path Wavelength selection panel Precondition R The UV detector is selected u Navigate to the Wavelength panel according the navigation path u Activate the checkbock next to UV u Tap the input box next to UV ð The display shows a dialog box with a numeric input box u Enter a value for the wavelength u Tap the button OK ð The dialog box closes ð The wa...

Страница 50: ...t is the maximum volume of the vial Navigation path Collection panel u Tap the radio button next to criteria you wish to use ð The criteria is selected u Tap the input box next to Peak ð The display shows a dialog box with a numeric input box u Enter a the volume you wish to collect u Tap the button OK ð The dialog box closes ð The value for the volume is saved u Tap the input box next to Non Peak...

Страница 51: ...lay shows the dialog Program collect u Tap the input box ð The display shows a dialog box with a numeric input box u Enter the time u Tap the button OK ð The time is saved ð The dialog box closes Add additional lines to the program collection u Tap the number field 1 ð The display shows a drop down list with selectable actions u Select if the line should be added above or below the selected line ð...

Страница 52: ...ween the low and high setting Low Used for volatile or semi volatile solvents Off The vapor sensor is off Navigation path Tools Vapor Sensor and Limits u Navigate to the Vapor Sensors dialog according to the navigation path ð The display shows the dialog box Vapor Sensors u Tap the drop down list next Vapor alarm sensitivity u Select the sensitivity value you wish to use u Tap the button Close ð T...

Страница 53: ...lvent Definition u Navigate to the Solvent Definition dialog according to the navigation path ð The display shows the dialog box Solvent Definition u Tap the button Add Solvent ð The display shows the dialog box Solvent u Tap the input box next to Name ð The display shows a dialog with an alphanumeric input box u Enter the name for the solvent u Tap the button OK ð The dialog box closes u Tap the ...

Страница 54: ...a solvent Navigation path Tools Solvent Definition u Navigate to the Solvent Definition dialog according to the navigation path ð The display shows the dialog box Solvent Definition u Select the solvent you wish to delete u Tap the button Delete Solvent u Answer the secure question with Yes ð The solvent is deleted 6 4 Tasks during a separation 6 4 1 Introducing a sample into the prep system u Con...

Страница 55: ...ct the syringe at the inert loop port 6 4 2 Injecting a sample into the flash system Injecting a sample into the flash system at the luer port CAUTION Not removed syringe after injection A not removed syringe after injection can lead to spill of solvent and injuries u Remove the syringe after injection All manuals and user guides at all guides com ...

Страница 56: ... the syringe at the luer port u Press the plunger u Disconnect the syringe at the luer port Injecting a sample into the flash system with a solid loader NOTE Removing is done in reverse sequence u Loosen the solvent line on the top of the cartridge holder All manuals and user guides at all guides com ...

Страница 57: ...idge NOTE Removing is done in reverse sequence NOTE Instead of a cartridge a bypass can be installed NOTE The automatic reading of the cartridge information can only be done with specific BUCHI RFID tagged cartridges u The indication for recognizing the cartridge is a sound u Hold the cartridge above the RFID reader u Wait until the instrument has taken over the cartridge data All manuals and user...

Страница 58: ...artridge holder u Install the cartridge by pushing the cartridge inlet down into the luer fitting at the bottom plate u Lower the upper part of the cartridge holder over the luer connection u Align it u Firmly press the arm in place to ensure a proper seal 6 4 4 Installing and removing a column NOTE Removing is done in reverse sequence All manuals and user guides at all guides com ...

Страница 59: ... Instruments 59 102 u Install the column in the bottom plate of the column holder u Lower down the upper part of the column holder u Remove the tubing from the upper part of the column holder u Attach the tubing to the column All manuals and user guides at all guides com ...

Страница 60: ...e tubing to the column 6 4 5 Inserting the fraction collection tray NOTE By default the first available tube on each tray is reserved for waste Changing the setting See Chapter 6 4 7 Selecting values on the dialog box Sample Loading page 61 1 2 1 Tab 2 Optical sensor slot u Place the collection tubes in the collection tray All manuals and user guides at all guides com ...

Страница 61: ...Sample Loading during the starting phase of a separation The following settings are possible Action Explanation Lower flow rate for specified time after in jection Reduce flow rate for a specified period when the pressure increases significantly at the start of the run after sample injection Flush 2nd solvent at the end of the run Automatically cleaning the system and column Override Start Vial Se...

Страница 62: ...ge 29 u Open the protection shield u Place the fraction collection trays inside the instrument See Chapter 6 4 5 Inserting the fraction collection tray page 60 u Close the protection shield u Open an existing method See Chapter 6 4 6 Selecting an existing method page 61 u Tap the button OK u Tap the button Start on the Run panel u According the requirements adjust the file name u Tap the button OK...

Страница 63: ...eted u Follow the instructions on the display ð Purging the instrument with air See Chapter 7 8 Removing solvent from an used cartridge page 78 ð Removing the cartridge See Chapter 6 4 3 Installing and removing a cartridge page 57 6 5 6 Shutting down the instrument Navigation path File Precondition R The separation process has ended u Purge the instrument with purging solvent See Chapter 7 6 Clean...

Страница 64: ... the fraction collection trays inside the instrument See Chapter 6 4 5 Inserting the fraction collection tray page 60 u Close the protection shield u Select a cartridge See Chapter 6 2 1 Selecting a cartridge Flash mode page 42 u Tap the drop down list next to A on the Solvent Selection panel ð The display shows a drop down list with the assigned solvents u Tap the required solvent ð The solvent i...

Страница 65: ... extends automatically by 5 minutes if the baseline at the end of the separation is not below the set threshold Precondition R The display shows the dialog box Separation End u According to the requirements extend the separation time by 5 minutes Precondition R The display shows the dialog box Run completed u Follow the instructions on the display ð Purging the instrument with air See Chapter 7 8 ...

Страница 66: ...e Chapter 6 7 1 Preparing the instrument page 66 R The required solvents are connected and assigned See Chapter 5 7 Assigning solvent to solvent lines page 26 R The sample is prepared R The waste bottle is empty u Calibrate the solvent level sensor See Chapter 5 8 Calibrating the solvent level sensor page 27 u Calibrate the waste level sensor See Chapter 5 12 Calibrating the waste level sensor pag...

Страница 67: ... panel page 39 Auto gradient hold See Chapter 6 1 6 Gradient panel page 39 6 7 5 Ending a Prep separation NOTE The separation extends automatically by 5 minutes if the baseline at the end of the separation is not below the set threshold Precondition R The display shows the dialog box Separation End u According to the requirements extend the separation time by 5 minutes 6 7 6 Shutting down the inst...

Страница 68: ...le is prepared R The waste bottle is empty u Calibrate the solvent level sensor See Chapter 5 8 Calibrating the solvent level sensor page 27 u Calibrate the waste level sensor See Chapter 5 12 Calibrating the waste level sensor page 29 u Open the protection shield u Place the fraction collection trays inside the instrument See Chapter 6 4 5 Inserting the fraction collection tray page 60 u Close th...

Страница 69: ...E Parameters that can be edited are highlighted in green Possibilities to edit the gradient during a separation Change the gradient See Chapter Editing the gradient in graphic mode page 47 Hold the gradient See Chapter 6 1 6 Gradient panel page 39 Auto gradient hold See Chapter 6 1 6 Gradient panel page 39 6 8 5 Ending a Prep separation NOTE The separation extends automatically by 5 minutes if the...

Страница 70: ...wn 6 9 Identifying fractions 6 9 1 Identifying fractions by peak Fig 9 Identifying fractions Navigation path Gradient panel Precondition R A separation is finished u Navigate to the Gradient panel according the navigation path u Tap the peak with the target value ð The Collection panel shows the corresponding vial All manuals and user guides at all guides com ...

Страница 71: ... the navigation path u Tap the target vial ð The Gradient panel shows the corresponding peak 6 10 Importing and exporting data 6 10 1 Printing a run report Navigation path File Print Run Report Precondition R The instrument is in past run mode u Navigate to the Print Run Report dialog according to the navigation path ð The display shows the Windows print dialog u Select your printer u Tap the butt...

Страница 72: ...ata to USB ð The instrument saves an Excel file to the USB storage device u Confirm the complete message ð The data is stored 6 10 3 Sending PDF to USB Navigation path Run panel Precondition R The instrument is in past run mode u Connect a USB storage device to the instrument u Navigate to the Run panel according the navigation path u Tap the button PDF to USB ð The instrument saves a PDF file to ...

Страница 73: ...leaks If a leak is observed resolve the issue before continuing Daily Fittings u Inspect fittings if solid material is deposited on a fitting clean and tighten replace the fitting before continuing Daily Drain lines u Check all drain lines to ensure that liquid can flow through them to the waste container Daily Waste bottle u Empty the waste bottle Daily Filters u Check the filters in the solvent ...

Страница 74: ...Confirm the secure question ð The Pure software is shutting down ð The display shows a Windows system u Connect a USB storage device to the instrument u Open the Windows Explorer u Navigate to the data you wish to backup See Chapter 10 2 Folder locations page 96 u Copy the needed data to the USB storage devices 7 3 Calibrating the display Navigation path Tools Calibration and Defaults u Navigate t...

Страница 75: ...izer u Disconnect the gas inlet from the nebulizer u Remove the knurled screws u Remove the nebulizer from the drift tube u Place the nebulizer in a beaker filled with 50 50 methanol water solution u Sonicate the nebulizer in an ultrasonic bath for 10 minutes u If the nebulizer is still completely blocked connect a high pressure air line to the nebulizer inlet to help remove the blockage u If the ...

Страница 76: ...quid and gas inlet lines to the nebulizer 7 5 Cleaning the check valve NOTE Most check valve problems can be solved by pumping a strong solution of liquid laboratory grade detergent through the check valves at a rate of 20 mL min for one hour Pumping detergent through the check valves Materials needed Liquid Laboratory Detergent Isopropanol Water 50 50 or Methanol Water 50 50 All manuals and user ...

Страница 77: ...ect the mobile phase outlet tubing from the top of the pump head using the 5 16 open end wrench u Remove both check valve housings from the pump head using the open end wrench ð The check valve capsule is accessible u Sonicating the check valve for 10 min in the appropriate solvent u Install the check valve u Install the check valve housings back into the pump head u Tighten the check valve housin...

Страница 78: ... a bypass See Chapter 6 4 4 Installing and removing a column page 58 u Navigate to the Solid Loader Flushing dialog according to the navigation path 7 8 Removing solvent from an used cartridge Navigation path Tools Manual Control Air Purging Precondition R The cartridge is installed u Navigate to the Air Purging dialog according to the navigation path u Enter the purging time according to your nee...

Страница 79: ...interrupted Power Supply line The touch screen is not responsive The touch screen is out of cali bration u Recalibrate the touch screen 8 2 Faults possible causes and solutions cartridge Malfunction Possible cause Solution Cartridge is not de tected RFID tag is not facing the RFID reader u Turn cartridge so that RFID tag faces RFID reader RFID tag is bad u Use new cartridge 8 3 Faults possible cau...

Страница 80: ... such as diethyl ether and methylene chloride u Premix the solvents to reduce solvent volatility u Place the highly volatile solvent bottle in an ice bath to eliminate boiling System pump pres sure is higher than expected Blocked solvent lines u Find the blocked lines and replace it Over tightened fitting u Loosen the fitting or replace it Blocked columns or fluidic path u Locate the component tha...

Страница 81: ...ten it up 8 5 Faults possible causes and solutions fraction collection Malfunction Possible cause Solution Liquid not being col lected in fraction tubes Incorrect fraction collection settings u Verify that fraction collection information is set properly Liquid not centered in fraction tube Fraction collector is not cali brated u Recalibrate the fraction collector Fraction collector FC arm does not...

Страница 82: ... that there is gas flow to the ELSD sampling valve Sample stuck on column u Use stronger solvent or change column chemistry ELSD not equilibrated long enough u Restart run to re stabilize and rezero ELSD baseline Blocked nebulizer u Sonicate nebulizer to clean or replace the nebulizer Blocked ELSD line u Trace the blockage and replace the blocked line Rotor and or stator in ELSD Sampling valve is ...

Страница 83: ... exhaust u Install the exhaust correctly See Chapter 5 9 Installing the exhaust ELSD only page 28 Electronics Preamp not grounded properly u Verify that preamp grounding cable is in place Nebulizer is partially blocked u Sonicate nebulizer to clean or replace Silica or packing material leaked out of cartridge u Replace the cartridge u Flush the system No UV signal UV light burned out u Replace the...

Страница 84: ...mp defect u Check the lamp and restart instrument u Contact BUCHI Customer Service No lamp Hardware error UV detector Instrument Alarm UV detector Bad power supply of lamp heater volt age Hardware error UV detector u Contact BUCHI Customer Service Instrument Alarm UV detector Bad power supply of lamp anodic volt age Hardware error UV detector u Contact BUCHI Customer Service Instrument Alarm UV de...

Страница 85: ...t peak caused by unworkable light in tensity Lamp defective Flow cell dirty u Check the lamp and restart instrument Instrument Alarm UV detector Low light intensity was found on some photo elements of CCD sensor Hardware error UV detector u Contact BUCHI Customer Service Instrument Alarm UV detector Spon taneously failure on analog or digital power supply Hardware error UV detector u Contact BUCHI...

Страница 86: ...tware Solvent Defaults File The solvent in formation file and the default solvent information file are missing Software damaged u Uninstall and reinstall software Using Default Sol vent List System defaults used in stead of actual re source Solvent file has been deleted u Restart the instrument Solvent Pressure Pressure limit has been exceeded Blockage in the line u Remove column u Replace with by...

Страница 87: ... The pressure gauge is not set correctly u Check pressure gauge setting to make sure that it is set to deliver 2 5 L min gas flow Inlet Pressure with Air State off around 85 115 psi Inlet Gas Pressure Out of Range LOW PRESSURE Before run starts Blockage in the system u Check the gas lines in the system for blockages The pressure gauge is not set correctly u Check pressure gauge setting to make sur...

Страница 88: ...the alarm Use the instrument in a hood or well ventilated area with no open solvents near the system Vapor sensor no sig nal Vapor sensor is not connected or is defect u Check the cable to the vapor sensor Instrument Alarm Watchdog Timing communication issue in software If in setup mode u Restart the instrument u If in run mode u Press reset u Restart after the run is finish Solvent Pressure Solve...

Страница 89: ... button to stop the alarm u Contact BUCHI Customer Service Prep Injection Valve Failure Prep Injection Valve failed to switch Only Flash mode runs can be performed u Press Reset button to stop the alarm u Contact BUCHI Customer Service Low Disk Space The disk space on this unit is almost full u Back up and delete data u Contact BUCHI Customer Service Solvent Safety Sen sor alert More solvent requi...

Страница 90: ... 8 9 Replacing the shuttle valve rotor Materials needed Torx wrench T10 Pure sampling valve kit u Switch the On Off master switch to Off Precondition R Make sure that all tubings are marked for later installation u Remove all tubings u Loosen the screws with the Torx wrench u Remove the valve head u Remove the valve rotor All manuals and user guides at all guides com ...

Страница 91: ... Pure Chromatography Instruments 91 102 u Make sure that the holes in the new rotor and the pins inside the valve match u Attache the valve head to the instrument with screws u Attach all tubings to the instrument All manuals and user guides at all guides com ...

Страница 92: ...vice 9 2 Disposal The operator is responsible for proper disposal of the instrument u When disposing of equipment observe the local regulations and statutory requirements regarding waste disposal u When disposing observe the disposal regulations of the materials used Materials used see Chapter 3 5 Technical data page 18 9 3 Returning the instrument Before returning the instrument contact the BÜCHI...

Страница 93: ...ck type 1 Rack for 12 x 75 mm glass tubes 1 pc 11066672 Pure rack type 2 Rack for 13 x 100 mm glass tubes 1 pc 11066673 Pure rack type 3 Rack for 16 x 125 mm glass tubes 1 pc 11066674 Pure rack type 4 Rack for 16 x 150 mm glass tubes 1 pc 11066675 Pure rack type 5 Rack for 18 x 150 mm glass tubes 1 pc 11066676 Pure rack type 6 Rack for 25 x 150 mm glass tubes 1 pc 11066677 Pure rack type 7 Rack fo...

Страница 94: ... Pure column holder XL Column holder for column diameters 50 to 70 mm 11068467 Pure nebulizer set 11069464 10 1 2 Sample introduction accessories Order no Pure Solid loader S set incl adapter set sleeve tubes 20 pcs and frits 40 pcs 11068975 Pure Solid loader M set incl adapter set sleeve tubes 20 pcs and frits 40 pcs 11070505 Pure Solid loader frits S 40 pcs 11068969 Pure Solid loader frits M 40 ...

Страница 95: ...8 044867 Pure transfer line ELSD 11069409 Touch screen stylus 11068360 Sample injection stator 11069688 10 1 4 Tubes Order no Pure Glass Tubes 13 x 100 mm 1000 pcs 148623414 Pure Glass Tubes 16 x 150 mm 1000 pcs 148623416 Pure Glass Tubes 18 x 150 mm 500 pcs 148623410 Pure Glass Tubes 25 x 150 mm 500 pcs 148623411 Pure Squared bottles 480 mL 24 pcs 148623412 10 1 5 Tools and adapter kits Order no ...

Страница 96: ...P 1 8 FEP PEEK 1 16 SS nut and ferrule Reductions Luer Lock adapters Fuses Injection valve 11068363 Pure solvent line kit Package with 4 solvent lines and 5 level sensing lines 11068215 10 1 6 Wear parts Order no Image Pure air filter 11066049 O Ring for Pure nebulizer 11066421 Pure nebulizer 11066423 Pure UV flow cell 0 3 mm For all C 8xx 11068210 Pure UV flow cell 1 3 mm For all C 8xx for enhanc...

Страница 97: ...aration data to provide a recommended gradient for silica separations of 2 or 3 components Specification of the samples needed Two different solvent concentrations Two separations Silica TLC plates Precondition R The display shows the dialog Navigator See Chapter 10 3 1 Open the navigator page 97 u Select the TLC Silica tab u Select the radio button for two or three components u Select the weaker ...

Страница 98: ...n is selected ð The display shows the default flow rate u Adjust the flow rate according to your needs u Select the radio button for Speed or Purity u Tap the button Calculate ð The display shows the results u Adjust the following values if necessary Column Flow rate Speed Purity u Tap the button Accept ð The gradient is saved for a run ð The dialog box closes 10 3 3 LC C18 The LC C18 Tool uses is...

Страница 99: ...elect the weaker solvent at drop down list A u Select the stronger solvent at drop down list B u Enter the solvent concentrations used in the HPLC separations u Enter the retention times for each separation under each chromatograph u Select the column you wish to use from the drop down list ð The column is selected ð The display shows the default flow rate u Adjust the flow rate according to your ...

Страница 100: ...the HPLC column type used from the drop down list u Enter the flow rate used in the HPLC separation u Enter the following HPLC gradient conditions lower and higher B times t1 t2 and t3 u Select the cartridge you wish to use for the Flash separation from the drop down list ð The cartridge is selected ð The display shows the default flow rate u Adjust the flow rate according to your needs u Tap the ...

Страница 101: ...All manuals and user guides at all guides com ...

Страница 102: ... buchi com www buchi com in en Indonesia PT BUCHI Indonesia ID Tangerang 15321 T 62 21 537 62 16 F 62 21 537 62 17 indonesia buchi com www buchi com id in Japan Nihon BUCHI K K JP Tokyo 110 0008 T 81 3 3821 4777 F 81 3 3821 4555 nihon buchi com www buchi com jp ja Korea BUCHI Korea Inc KR Seoul 153 782 T 82 2 6718 7500 F 82 2 6718 7599 korea buchi com www buchi com kr ko Malaysia BUCHI Malaysia Sd...

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