Biotek ELx808, Руководство оператора

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Perform the Tests
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95
™
If any set of mean values is out of the expected range, review the
other three sets of mean values for the same channel pair. For
example, if the A1/H12 comparison fails (the wells are not within
the expected range of each other), review the comparisons of
A2/H11, H1/A12, and H2/A11. If two or more sets of mean values
for a channel pair are out of the expected range, there is a
problem with one of the eight read channels. If only one of the
four mean values results in a failure, check the well for debris and
the plate for scratches or fingerprints.
Accuracy Specification:
For comparison in this test, the following accuracy specification is applied,
using Normal reading mode and a 96-well microplate.
± 1.0% ± 0.010 OD from 0.000 OD to 2.500 OD at 405 nm
Perform Liquid Test 2
™
The recommended method of testing the instrument performance is to
use the Absorbance Test Plate to confirm alignment, repeatability, and
accuracy, which will also confirm linearity. If a Test Plate is not
available, Liquid Test 2 can be used for these tests.
Required Materials:
•
A new 96-well, flat-bottom microplate (Corning Costar #3590 is
recommended)
•
Ten test tubes, numbered consecutively, stored in a rack
•
Calibrated hand pipette (Class A volumetric pipette recommended)
•
Stock solution A or B (these are the same solutions as for Liquid Test 1)
•
A 0.05% solution of deionized water and Tween 20
Prepare Dilutions:
Create a percentage of dilution series, beginning with 100% of the original
concentrated stock solution (A or B) in the first tube, 90% of the original
solution in the second tube, 80% in the third tube, all the way to 10% in the
tenth tube. Dilute using the 0.05% solution of deionized water and Tween
®
20. This solution can also be made by diluting the BioTek wetting agent 200:1.
ELx808 Operator’s Manual