Bio-Tek FL600 Скачать руководство пользователя страница 12 | Manualshive

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This chapter introduces the Bio-Tek FL600 Fluorescence

Plate Reader. You'll find information on hardware and

software requirements and optional equipment

suggestions.

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Thank you for selecting the Bio-Tek FL600. The FL600 is a powerful
fluorescence and absorbance measurement system which measures
through the use of a photomultiplier tube housed inside of a light-
impermeable detection compartment. The FL600 is capable of quantifying
both soluble and cell-associated fluorescence. The system consists of:

•

The Bio-Tek FL600 Fluorescence Plate Reader

•

KC4™ reader control and data reduction software

•

FL600 System Software for diagnostics

•

Accessories Kit

•

Serial Communications Cable (P/N 75034)

Both software packages are for Windows-based PCs and provides the user
with full command of the operation of the X-Y scanner. FL600’s precision
X-Y mechanism scans a microplate with a sensitive optical detection
system to quantify the fluorescence or absorbance in each well.

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Содержание FL600

Страница 1: ...BIO TEK FL600 FLUORESCENCE PLATE READER HARDWARE GUIDE 29 D5 9 CDBE 5 DC 9 3...

Страница 2: ...29 D5 9 CDBE 5 DC 9 3 HIGHLAND PARK BOX 998 WINOOSKI VT 05404 0998 USA 800 451 5172 802 655 4040 FAX 802 655 7941...

Страница 3: ...BIO TEK FL600 FLUORESCENCE PLATE READER For Research Development Use Only HARDWARE GUIDE Part No 6001000 June 1998 Revision D...

Страница 4: ...anscribed stored in a retrieval system or translated into any language without the written permission of Bio Tek Instruments Incorporated Trademarks Bio Tek is a registered trademark and FL600 and KC4...

Страница 5: ...d instructions for Setting Up the FL600 Reader Page 5 2 Changed electrical specification for fuses Page 5 11 Modified text and data on serial communications to reflect parity differences between KC4 r...

Страница 6: ...anty is VOID if the Product has been repaired or altered by persons not authorized by Bio Tek or if the Product has had the serial number altered effaced or removed This Warranty is VOID if any of the...

Страница 7: ...ternal Voltage Always turn off the power switch and unplug the power cord before cleaning the outer surfaces of the instrument or changing filters Warning Liquids Avoid spilling liquids on the instrum...

Страница 8: ...uld use this instrument Read the manual carefully before operating this instrument Direct current Courant continue Gleichstrom Corriente continua Corrente continua Alternating current Courant alternat...

Страница 9: ...gleitpapiere Atenci n vease los documentos incluidos Attenzione consultare la doc annessa Caution risk of electric shock Attention risque de choc electrique Gef hrliche elektrische Spannung Atenci n r...

Страница 10: ...5 Running System Diagnostics 2 7 What to Do if the Tests Fail 2 8 Exiting the FL600 Diagnostic Software 2 9 3 Replacing Filters and Adjusting Probe Height Adding and Replacing Filters 3 1 Changing the...

Страница 11: ...ditions 5 1 Electrical Specifications 5 2 Optical Specifications 5 2 Scanning Mechanism 5 3 Temperature Control 5 4 Performance Specifications 5 4 CE Mark Approval 5 8 Computer Specifications for FL60...

Страница 12: ...light impermeable detection compartment The FL600 is capable of quantifying both soluble and cell associated fluorescence The system consists of The Bio Tek FL600 Fluorescence Plate Reader KC4 reader...

Страница 13: ...in list of industry standard plates or they can define their own unique plate format s The FL600 is designed to meet international radio frequency emission standards EMC immunity standards and user s...

Страница 14: ...ity floppy disk drive 3 1 serial COM and 1 parallel LPT port Mouse and keyboard ports Microsoft or compatible mouse 5HFRPPHQGHG RPSXWHU 5HTXLUHPHQWV IRU 6 VWHP 6RIWZDUH DQG 6RIWZDUH IRU LQGRZV DQG IBM...

Страница 15: ...ory 640 KB base extended to 16 MB minimum 250 MB hard drive 3ULQWHU Microsoft Windows compatible printer 6RIWZDUH 5HTXLUHPHQWV FL600 System Software version 2 or higher KC4 reader control and data red...

Страница 16: ...computer s serial port installing the software and setting up communications parameters One of the serial ports most likely already has a mouse connected to it the FL600 software since it operates und...

Страница 17: ...ported QVWDOOLQJ Please consult your KC4 User s Guide Place disk 1 into the floppy drive Run Setup exe by choosing the Run option from the File Menu of your Windows program This routine will guide you...

Страница 18: ...drive or the directory or both Setup creates a new group called Bio Tek FL600 Note that this is different from the KC4 group created by the KC4 software NOTE Be sure that TrueType Fonts are enabled i...

Страница 19: ...Step 1 To start the FL600 diagnostic software From within Windows double click on the FL600 icon Depending on your computer speed the FL600 diagnostic software should boot up from within 15 to 60 seco...

Страница 20: ...de of the front panel and check the COM Port Setting NOTE When the Reader has been switched on a green light next the switch lights up and the plate transport moves out ready to load a plate Step 1 Pu...

Страница 21: ...s not correctly connected it is not switched on the incorrect communications port is selected there is a cable problem Check that the reader is switched on and correctly connected to the computer that...

Страница 22: ...1 Pull down the Instrument menu and select Diagnostics You will see the following window Figure 2 5 Initial FL600 Diagnostics Screen Step 2 To start diagnostic tests click on the Start button Informat...

Страница 23: ...ader was will display on the screen Print the log by selecting the File menu and choosing Print Enter the requested details on the fax cover sheet which prints automatically with the log and fax both...

Страница 24: ...and have the door automatically closed click in the Close reader door check box and click on OK to exit the software and close the door make sure you have removed your plate Click Cancel to continue...

Страница 25: ...Getting Started 2 10...

Страница 26: ...step process Changing the excitation and emission or absorption filters in the filter wheels inside the reader Changing the settings to reflect the filter change in the KC4 The settings in the system...

Страница 27: ...reader s power cord Never operate the instrument with the cover off Operating the reader without a cover poses a serious shock hazard and may damage the detection circuit Exposing the inside measurem...

Страница 28: ...ls Locate the two filter assemblies at the rear of the reader adjacent to the printed circuit board Figure 3 3 Excitation Filter Wheel Cover Emission Filter Wheel Cover Figure 3 3 Filter wheel locatio...

Страница 29: ...ters remove the holder containing the filter not the filter itself Step 6 Ensure that the glass surfaces of the filters are free of dust and smudges Step 7 Insert the new filter and note its wavelengt...

Страница 30: ...rinted on the top or side of each filter cell 1 1 Y YW W U U d d 1 1b bb_g b_g Figure 3 5 Aligning the filter with the filter wheel Step 9 Replace the covers on the filter wheels Put any unused filter...

Страница 31: ...are filter settings to accurately reflect the new filters and their position on the wheel The values for filters in the KC4 software must match the values of the filters in the reader to avoid damagin...

Страница 32: ...truding from the top and bottom 5HPRYLQJ WKH LOWHU IURP WKH GDSWHU Step 1 Remove the filter adapter from the filter wheel as described in the section entitled Changing Filters Inside the Reader Step 2...

Страница 33: ...ured with a top probe height that will provide maximum sensitivity for the specified plate thickness Should you wish to change plate types it may be necessary to reset the probe height to prevent the...

Страница 34: ...edge of the plate and shim on the plate carrier Step 7 Turn the X axis and the Y axis drive screws so that the appropriate notch in the shim is located underneath the top probe Step 8 Carefully lower...

Страница 35: ...E W D OO R PO W ER SU PPLY TO P PRO BE M ICRO PLATE X A X IS SCRE W SET SCREW S TOP PROBE PROBE HEIGHT ADJUSTM ENT JIG SET SCRE W FL600 TO P PR OB E POSIT IO N side view TO P PR OB E A D JU STM EN T J...

Страница 36: ...cing its lamp RZ WR OHDQ WKH WHULRU Exterior maintenance consists of simply wiping the reader with a damp cloth and mild detergent RZ WR OHDQ DQG 5HSODFH WKH LU LOWHU Dust can affect flourescence valu...

Страница 37: ...and or rinsing by hand with sudsy water and then clean water Be sure to dry the filter thoroughly before reinserting it into the fan cover Step 4 If the filter is damaged or difficult to clean or if t...

Страница 38: ...as any residue could hamper illumination We recommend that you wear gloves when touching a new lamp Step 1 Make sure the plate transport mechanism is parked To do so press the red button in the recess...

Страница 39: ...ep 4 Remove the top cover and set it aside Figure 4 4 At the back of the FL600 on the left side you will find the lamp assembly as shown below Excitation Filter Wheel Emission Filter Wheel Lamp Assemb...

Страница 40: ...Figure 4 6 Step 9 Carefully remove the lamp assembly from the lamp housing Step 10 Remove the lamp from the lamp assembly by removing the three 3mm hex screws Step 11 Insert the new lamp P N 6000556S...

Страница 41: ...d re route the cables through the cable harness Step 17 Replace the reader cover and attach it by screwing in the five screws Step 18 Plug in the power cable and the computer cable Step 19 Turn on the...

Страница 42: ...cations for the Bio Tek FL600 Fluorescence Plate Reader LR 7HN OXRUHVFHQFH EVRUEDQFH 3ODWH 5HDGHU 3K VLFDO LPHQVLRQV Height 247 mm 97 in Width 707 mm 278 in Depth 426 mm 168 in Weight 23 kg 50 6 lb Sh...

Страница 43: ...bly Lamp Life 2000 hours minimum Excitation Range 300 nm 650 nm filters fitted in a motor driven wheel assembly Emission Range 350 nm 750 nm filters fitted in a motor driven wheel assembly complete wi...

Страница 44: ...ntrol The height of the top reading probe may need to be set by the user for different plate heights See the section entitled Adjusting Top Optical Probe Height in Chapter 3 6FDQQLQJ 0HFKDQLVP Speed 6...

Страница 45: ...thin 30 minutes after switching on Time to Cool Approx 2 5 hours from 37 Celsius to 6 Celsius above the on board ambient temperature reference sensor 3HUIRUPDQFH 6SHFLILFDWLRQV Standard Plate Types Al...

Страница 46: ...ing 5 0mm Probe 20 pg ml solution of Sodium Fluoroscein in PBS 300 l per well Signal to Noise Ratio of 2 or greater Excitation 485 20 Emission 530 25 Dynatech 96 well black plate 160 pg ml solution of...

Страница 47: ...1 read 3 seconds 2 filter sets using Fast Kinetic mode End Point Speed 48 seconds plate including in and out time using rapid mode in fluorescence Static Mode The probe will remain stationary under a...

Страница 48: ...n The references are 1 Black Acetal Reference Disk used for Optical Density 0 transmission and fluorescence background 2 Green Reference Disk for fluorescence with Excitation 485 and Emission 530nm 3...

Страница 49: ...pliance was conducted to the limits and methods of EN 50082 1 1992 IEC 1000 4 2 1995 EN 61000 4 3 1996 and IEC 1000 4 4 1995 U S A F C C C L A S S A Warning Changes or modifications to this unit not e...

Страница 50: ...et pas du bruits radioelectriques depassant les limites applicables aux appareils numerique de la Class A prescrites dans le Reglement sur le brouillage radioelectrique edicte par le ministere des Com...

Страница 51: ...er 640 KB base memory extended to 8 MB Color graphics monitor VGA Color graphics adapter VGA 120 MB hard drive High density floppy disk drive 3 1 serial and 1 parallel port Microsoft Windows compatibl...

Страница 52: ...data to computer 3 TX RX data from computer 4 DTR Not used 5 GND Signal ground 6 DSR Not used 7 RTS Linked to pin 8 8 CTS Linked to pin 7 9 RI Not used Serial Port Parameter KC4 Reader Control and Da...

Страница 53: ...nfigured for minimum switch time of pairs in kinetics applications using two color fluorescence FL500 AND FL600 EXCITATION EMISSION FILTERS Wavelength Bandwidth Part Number FL500 use std 420 700 nm ve...

Страница 54: ...02025 Both Standard Filter 590 35 5002024 Both Standard Filter 620 40 5002013 Both 630 32 5002014 Both 633 10 5002017 Both 635 10 5002019 Both 645 40 5002026 Both Standard Filter 680 30 5002029 Both E...

Страница 55: ...dapter System in Chapter 3 for further information For the latest additions please contact Bio Tek s Customer Service Department FL600 ABSORBANCE FILTERS Wavelength Part Number 2874 plus wavelength 34...

Страница 56: ...echnical Information 5 15 Wavelength Part Number 2874 plus Wavelength 580 2874580 590 2874590 600 2874600 610 2874610 620 2874620 630 2874630 640 2874640 650 2874650 660 2874660 720 2874720 750 287475...

Страница 57: ...5 16 Technical Information...

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