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Содержание Cielo AIQ030

Страница 1: ...Azure Cielo Real Time PCR Systems User Manual Part Numbers AIQ030 and AIQ060...

Страница 2: ...d to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment...

Страница 3: ...e Biosystems or the company s authorized agent Use of spare parts supplied by anyone other than Azure Biosystems Damage caused by accident or misuse Damage caused by disaster Corrosion caused by impro...

Страница 4: ...um Informationen hinsichtlich der Entsorgung Ihres Ger tes zu erhalten ITA Questo simbolo indica che i rifiuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come...

Страница 5: ...net Network 11 2 2 Connect Azure Cielo Real Time PCR system to PC via Azure Cielo Manager software 11 3 Device Software Overview 14 3 1 The Home Screen 14 3 2 Experiment Setup 14 3 2 1 Protocol Manage...

Страница 6: ...10 50 L recommended 5 150 L 10 50 L recommended Excitation source LED LED Detection channels 3 6 Multiplexing Up to 3 targets Up to 6 targets Thermal element Peltier Peltier Max block ramp rate 6 C se...

Страница 7: ...tic handling Azure Cielo Real Time PCR Systems cannot accommodate a fully skirted 96 well plate Azure Cielo Systems are compatible with only a non skirted or semi skirted 96 well plate Plate color can...

Страница 8: ...ltra clear The Azure Cielo Systems are not compatible with domed caps for tubes or plates C Only a few samples While the Cielo Real time PCR system is designed for 96 well plates many times researcher...

Страница 9: ...or call 925 307 7127 9am 4pm Pacific time Figure 2 Filled orange circles show locations of samples across the 96 well format There are a total of six samples in tubes Panel A shows an uneven distribu...

Страница 10: ...be placed away from interfering electrical signals and magnetic fields If possible a dedicated electrical outlet should be used to eliminate electrical interference from other instrumentation in your...

Страница 11: ...the SEARCH DEVICES button to locate available Azure Cielo Real Time PCR systems within the Wi Fi network Select the instrument to be used and then CONNECT The designated instrument and the PC should n...

Страница 12: ...witch on the instrument using the power switch on the back Wait until the front power button light shows green Once green is visible on the front press and hold the front power button for eight second...

Страница 13: ...e should immediately be removed from the USB port on the front Figure 9 Once the instrument has successfully updated the software it will instruct the user to remove the USB drive and select OK to reb...

Страница 14: ...rotocol see Section 3 2 Data Manager Access run data see Section 3 3 Device Settings Access device settings see Section 3 4 Connection Bar Status and strength of internet connection Table 1 Buttons in...

Страница 15: ...OTOCOL MANAGER screen under Experiment Setup Button Button Name Function Home Back to Home Screen Connection Bar Status and strength of internet connection Sort Sort in ascending or descending order A...

Страница 16: ...can see the new user added to the PROTOCOL MANAGER screen Figure 13 Add New User process flow C Add New Protocol Tap symbol to add a new protocol Tap on Add Protocol to add a new user Enter the name f...

Страница 17: ...delete all existing protocols and data stored under that user The system will prompt you to confirm the deletion Tap OK to delete or CANCEL to abort Figure 16 Delete exisiting user or protocol in PRO...

Страница 18: ...THER button to customize reaction volume Lid Temperature Select lid temperature default setting is 105 C Off lid heating Turn off lid heating Light Source Select light source s Back Back to PROTOCOL M...

Страница 19: ...empty if turned off see images below To deselect wells tap on the well to clear selection Wells are deselected in sets of two columns Press OK to save changes on to the plate and return to PROPERTIES...

Страница 20: ...p and run protocol Email notification with results attached will be sent when the protocol run is successfully completed 3 2 5 Run Setup Figure 23 RUN SETUP screen Button Button Name Function Home Bac...

Страница 21: ...s will delete the highlighted step Go To module Send the program to a cycling parameter in a previous part of the protocol Save This will save the Run settings Camera and or Gradient PCR Advance setti...

Страница 22: ...f the last step All parameters are adjustable and can be changed Additional steps may also be added if something other than a two step protocol is required Tapping the GO TO option will insert two add...

Страница 23: ...ra icon again and the icon will return to the inactive state light gray Alternatively within the step where detection needs to be active tap on the settings icon A dialog box will open with a toggle s...

Страница 24: ...he MODIFY button to retain the new exposure setting or CANCEL to abort it Figure 25 Select channel to be adjusted The switch button turns BLUE when the camera is ON and GRAY when the camera is OFF The...

Страница 25: ...olumn across the plate Users are not able to alter those assigned temperatures and a minimum difference of 1 degree is required for the temperature range The GRADIENT option can be accessed by opening...

Страница 26: ...ssed over a short amount of time adjusting to a faster ramp rate could significantly shorten the overall run time If a particularly long or high GC content primer is in use a slower ramp rate might en...

Страница 27: ...at 1 C this means for every increase of temperature of 1 C the plate will be scanned and data will be recorded Higher resolution means more scans are performed acquiring more data with each scan 0 1 C...

Страница 28: ...re 31 The screen display after RUN button activated The run time elapsed and remaining can be monitored using the status box near the bottom of the run screen The green meter indicates how much of the...

Страница 29: ...een displays the percentage of experiment completed and time remaining At the initiation of a run the instrument will provide a default experiment file name and a location to store the data The user c...

Страница 30: ...tatus and strength of internet connection Sort Sort in ascending or descending order Delete Delete User Delete Protocol Delete Experiment Export Export Data Table 6 Buttons in the DATA MANAGER screen...

Страница 31: ...ation curve window within the preview area The Amplification Curve data will be shown as seen in Figure 36 Color coded buttons to the left of the plot window correspond to all the channels available w...

Страница 32: ...graphs to be visible or hidden To return to the DATA MANAGER screen close the viewing windows by clicking on the CLOSE option at the very top right of the plot window B Delete User From the listed us...

Страница 33: ...xported to a USB drive using one of the two ports on the instrument Data can be exported via email To email files please verify the following Cielo device has been successfully connected to the Intern...

Страница 34: ...ame is listed The device name can be renamed by the end user at their discretion The current system status is available on this page and provides information related to the readiness state of the syst...

Страница 35: ...tant Make sure that the Cielo is successfully connected to the Internet To connect the Cielo Device to the internet please refer Section 2 3 Connect Azure Cielo Real Time PCR system to internet networ...

Страница 36: ...m Port 587 Encryption STARTTLS MSN Server imap mail outlook com Port 993 Encryption SSL TLS Server pop mail outlook com Port 995 Encryption SSL TLS Server smtp mail outlook com Port 587 Encryption STA...

Страница 37: ...ings Verify information has been entered correctly c Restart the device and try again 7 It is important follow these instructions in the order provided to enable the Email feature in the Protocol Setu...

Страница 38: ...ny double stranded DNA fragments in an amplification reaction including nonspecific targets such as primer dimers By running a melt or dissociation curve at the completion of the run specificity of th...

Страница 39: ...ding dye It will bind to any double stranded DNA molecule If there are primer dimer interactions or other off target primer to template binding SYBR Green will not distinguish between the desired targ...

Страница 40: ...ndations on the best quencher to use with the fluorescent dye molecule chosen for detection While the probe is intact the quencher molecule is adjacent to the 5 end of the probe where the detection fl...

Страница 41: ...on two different instruments the final data analysis results could be compared For example if an investigator was running a standard curve and then determining a copy number of a target that result c...

Страница 42: ...ure Biosystems logo Azure Biosystems and Cielo are trademarks of the Company All other trademarks service marks and trade names appearing in this brochure are the property of their respective owners U...

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