Azure Cielo Real-Time PCR Systems User Manual
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2. Time settings:
• Tap on the time display button for each cycle to alter the time spent at that step. Time can
be entered in either minutes or seconds. Enter the time using the key pad on the screen.
Example:
To set the time to 2 minutes, select Min on the screen and enter the number 2
using the key pad on the screen. Press DONE to save.
Example 2:
To set time to 1 minutes 30 seconds, tap on Min on the screen and enter the
number 1. Tap on Sec and enter the number 30 using the key pad on the screen. Press
DONE to save.
• Tap C to clear the entered numerical digits.
3. Go To module and Cycle Settings:
The Go To module and the Cycle Settings are closely linked. Regardless of which step is
highlighted, by tapping on the GO TO button, a two-step set of conditions will be added to the
end of the run (but before the melt curve portion of the protocol). The first step is a 95ºC for 10
seconds followed by a 60ºC for 10 seconds. A repeat cycling default of 30 cycles will also be
visible in a small purple oval with an arrow indicating to which step of the cycle it will go after
completion of the last step. All parameters are adjustable and can be changed. Additional
steps may also be added if something other than a two-step protocol is required
• Tapping the GO TO option will insert two additional steps at the end of the protocol. The
cycles will be repeated for a default of 40 times. User can freely add or delete steps with
the and buttons and adjust temperature and time for these new steps
• Tap on the purple display button
at the end of the CYCLING portion of the RUN
SETUP display. The number listed in this purple oval is how many times the conditions
will be repeated. By tapping on the purple display, the number of repeats (cycles) and the
GO TO location can be adjusted (GO TO which step). Be sure to tap the MODIFY button to
save the changes.
• The default setting is for two-step cycling at 95ºC and 60ºC. The default experiment is set
with camera ON (to detect) at the end of the 60ºC step. Please note, if steps are deleted
or added, the default camera setting is OFF. Please review the camera settings to ensure
detection is ON and is happening at the appropriate step.
Example:
To set 40 cycles consisting of step 2 and 3, tap on the purple button that
displays number of cycles. A dialogue box will open, and the number of cycles can be
adjusted as well as the step to GO TO.
Figure 24. Go To module on the
RUN SETUP screen.