Azure Cielo Real-Time PCR Systems User Manual
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A. Access run data (Amplification Curve and Temperature Curve)
• By selecting the user, protocol and the data file name, the corresponding Amplification Curve and
Temperature Curve will appear to the right in a PREVIEW window.
Figure 35. The Amplification Curve and
Temperature Curve are accessible on DATA
MANAGER screen upon selecting the data name.
Figure 37. Temperature Curve.
1. Amplification Curve
• To examine the Amplification Curve more closely, click on the amplification curve window
within the preview area. The Amplification Curve data will be shown as seen in Figure 36.
• Color coded buttons to the left of the plot window correspond to all the channels available
within that run file. The user can select individual amplification traces by selecting one specific
channel from the options presented at the left of the plot window.
• Each of the color coded buttons will toggle between showing the raw data plot associated
with that dye channel, and turning off the dye channel to simplify viewing of the raw
data plots.
• To return to the DATA MANAGER screen, close the viewing windows by clicking on the CLOSE
option at the very top right of the plot window.
Figure 36. Amplification Curve.