Azure Cielo Real-Time PCR Systems User Manual
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Button
Button Name
Function
Pause
Pause the run
Run
Start the run or continue the run after pause
View curves
View amplification and temperature curves of the run
Export
Export data file to USB drive
Back
Go back one screen. Selection of this button will return user
to the RUN screen.
Table 5. Icons and buttons in RUN screen of a selected protocol.
• If at any time the user wishes to stop the experiment, select the STOP
icon.
• Selection of the pause icon will pause the experiment. Once paused, the button changes to
a RUN button . Press the RUN button to resume the run.
• The progress bar at the bottom of the screen displays the percentage of experiment
completed and time remaining.
• At the initiation of a run, the instrument will provide a default experiment file name and
a location to store the data. The user can select an alternative experiment file name or
destination folder. Whether retaining the default options or customizing, the instrument will
automatically save the AZD raw data experiment file as selected at the completion of the run.
• LID TEMP and SAMPLE TEMP provide information regarding the lid and sample temperatures
at any point during the run. These can be found to the left of the status bar.
• To view the amplification curve and temperature curve during the PCR run, select the VIEW
CURVES icon from the menu. The following screen will appear. Tap the BACK button
to
return to the RUN screen.
Figure 33. Amplification curve and temperature
plot during a run.