Ankom ANKOM200 FIBER ANALYZER, Руководство оператора

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Operator’s Manual
Rev 1/24/20
pg. 23
Crude Fiber Analysis
Crude Fiber Calculation
Crude Fiber contained within a food or feed sample can be calculated using the following formula:
% Crude Fiber = 100 x (W
3
– (W
1
x C
1
))
W
2
Where: W
1
= Bag tare weight
W
2
= Sample weight
W
3
= Weight of Organic Matter (loss of weight on ignition of bag and fiber)
C
1
= Ash corrected blank bag factor (running average of loss of weight on
ignition of blank bag / original blank bag)
Crude Fiber Sample Preparation Procedure
To prepare samples for fiber analysis, follow the procedure detailed below.
IMPORTANT:
When using the ANKOM
200
Fiber Analyzer for Crude Fiber analysis, at least one
blank filter bag should be included with the sample set as an indicator of particle
loss. A running average of the blank bag weights is used in the fiber calculation as
the C
1
correction factor. A C
1
value larger than 1.0000 indicates that sample particles
were lost from filter bags and deposited on the blank bag(s). Any fiber particle loss
from the filter bags will generate erroneous results. If particle loss is observed, the
grinding method should be evaluated.
1. Using a Solvent Resistant Marker, number all of the filter bags you will use during the fiber
analysis.
2. Weigh and record the weight of each empty filter bag (W
1
).
3. Set the Heat Sealer dial to between 4 and 5. (The setting may vary from sealer to sealer.)
4. Seal at least one empty filter bag (to be used as a blank) within 4mm
of its open end. Keep the sealer arm down for 2 – 3 seconds after
the red sealer light turns off (to cool the seal). The seal can be seen
as a solid melted stripe along the top edge of the filter bag (as shown
to the right). If the seal is not strong, re-seal the bag.
5. Place an empty filter bag in the Bag Holder in an open position.
6. Tare the weight of the empty filter bag and the holder together.
7. Add 0.95 – 1.00g of sample to the filter bag. Keep all particles away from the sealing area
of the filter bag.
8. Record the weight of the sample (W
2
).
Seal