Ankom ANKOM200 FIBER ANALYZER, Руководство оператора

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Operator’s Manual
Rev 1/24/20
pg. 19
NDF Analysis
NDF Calculation
NDF contained within a food or feed sample can be calculated using the following formula:
% NDF (as-received basis) = 100 x (W
3
– (W
1
x C
1
))
W
2
Where: W
1
= Bag tare weight
W
2
= Sample weight
W
3
= Dried weight of filter bag with fiber after extraction process
C
1
= Blank bag correction (running average of final oven-dried weight
divided by original blank bag weight)
NDF Sample Preparation Procedure
To prepare samples for fiber analysis, follow the procedure detailed below.
IMPORTANT:
When using the ANKOM
200
Fiber Analyzer for NDF analysis, at least one blank
filter bag should be included with the sample set as an indicator of particle loss. A
running average of the blank bag weights is used in the fiber calculation as the C
1
correction factor. A C
1
value larger than 1.0000 indicates that sample particles were
lost from filter bags and deposited on the blank bag(s). Any fiber particle loss from
the filter bags will generate erroneous results. If particle loss is observed, the grinding
method should be evaluated.
1. Using a Solvent Resistant Marker, number all of the filter bags you will use during the fiber
analysis.
2. Weigh and record the weight of each empty filter bag (W
1
).
3. Set the Heat Sealer dial to between 4 and 5. (The setting may vary from sealer to sealer.)
4. Seal at least one empty filter bag (to be used as a blank) within 4mm
of its open end. Keep the sealer arm down for 2 – 3 seconds after
the red sealer light turns off (to cool the seal). The seal can be seen
as a solid melted stripe along the top edge of the filter bag (as shown
to the right). If the seal is not strong, re-seal the bag.
5. Place an empty filter bag in the Bag Holder in an open position.
6. Tare the weight of the empty filter bag and the holder together.
7. Add 0.45 – 0.50g of sample to the filter bag. Keep all particles away from the sealing area
of the filter bag.
8. Record the weight of the sample (W
2
).
Seal