Ankom ANKOM200 FIBER ANALYZER, Руководство оператора

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Operator’s Manual
Rev 1/24/20
pg. 21
NDF Analysis Procedure using the ANKOM
200
Fiber Analyzer
To perform NDF analysis on prepared samples, follow the procedure detailed below.
IMPORTANT:
The Vessel must be at room temperature before beginning an NDF analysis.
1.
Verify that the Exhaust Hose is connected to the instrument and securely positioned in the drain.
2. Turn the instrument Power Switch to the ON position.
3. Lift the Lid Handle to open the Vessel Lid.
4. Place the Bag Suspender with the samples and the Bag
Suspender Weight into the Vessel.
5. Pour a maximum of 2 L of Neutral Detergent (ND)
solution into the Vessel along with 20 g of sodium
sulfite (0.5 g / 50 ml of ND solution) and 4.0 ml of α-
amylase.
6. Press the
HEAT
and
AGITATE
buttons on the front of
the instrument so they are lit. Look in the Vessel to
confirm agitation.
7. Close the Vessel Lid.
8. On the Timer/Clock, set the Timer to 75 minutes and
press the Start/Stop button.
9. When the Timer sounds, the extraction process is
complete. Press the
HEAT
and
AGITATE
buttons to turn
them off (the buttons will not be lit when they are off).
Open the Exhaust Valve (slowly at first) and exhaust
the hot solution BEFORE opening the Vessel Lid.
IMPORTANT:
Because the Vessel is under pressure, the Exhaust Valve must be opened to release
the pressure and the solution BEFORE opening the Vessel Lid.
10. After the solution has been exhausted, open the Vessel Lid slowly to allow air to push any remaining liquid out the
Exhaust Hose.
11. Close the Exhaust Valve.
12. Add 1900 ml - 2000 ml of 70°C - 90°C rinse water and 4.0 ml of α-amylase.
IMPORTANT:
During the rinse process, if the
HEAT
button is OFF, the Vessel Lid can be open. If
the
HEAT
button is ON, the Vessel Lid must be closed.
13.
Press the
AGITATE
button.
14. On the Timer/Clock, set the Timer to 5 minutes and press the Start/Stop button.
15. When the Timer sounds, the rinse is complete. Slowly open the Exhaust Valve to drain the hot water.
16. Repeat steps 12 - 16 to accomplish the second rinse.
17. Repeat steps 12 - 16
without adding α-amylase
to accomplish the third rinse.
18. After the third rinse, open the Vessel Lid and remove the Bag Suspender from the Vessel.
19. Remove the bags from the Bag Suspender trays and place them in an appropriately sized beaker.
Agitate Button
Heat Button
Timer/Clock
Temperature
Controller
Power Switch
(out of view)
Exhaust Hose Connector
Exhaust Valve Handle
Lid Handle
Vessel Lid
Vessel