Amersham Pharmacia Biotech AKTA design pH/C-900, Руководство пользователя

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2.4 Installing the pH electrode (optional in ÄKTApurifier systems)
Mounting the flow cell holder
1 Hook the flow cell holder on the right hand
side of the housing. Secure it with the slide
clamp.
If the flow cell holder is not used, the flow
cell must still be installed at an angle of 30°
from the vertical with the outlet placed
higher than the inlet to prevent air bubbles
being trapped in the cell.
The flow direction is marked on the flow cell.
2 Connect the tubing with the “Fingertight” connectors.
Inserting the pH electrode
Note: Handle the pH electrode with care.
1 Unpack the pH electrode. Ensure that it is not broken or dry.
2 Prior to first using the electrode, remove the electrode end cover
and immerse the glass bulb in buffer for 30 minutes.
3 Remove the dummy electrode from the
flow cell and store it in the flow cell
holder.
4 Carefully insert the electrode in the
flow cell. Tighten the nut by hand to
secure the electrode.
Note: If the electrode is not fully
inserted, the system will leak and a
dead volume will occur in the holder.
5 Connect the pH electrode cable to the
rear of the instrument to the socket
pH
Probe
.
CAUTION! The tip of the pH electrode consists of a thin glass
membrane. Protect it from breakage, contamination and drying
out or the electrode will be destroyed. Always store the electrode
with the end cover filled with a 1:1 mixture of pH 4 buffer and
1 M KNO
3
. Do NOT store in water only.
Monitor pH/C-900
User Manual 18-1120-06 Edition AC
3
Installation
2
End
cover
To instrument
rear
”pH Probe”
Electrode
Flow direction
Dummy
Flow cell
holder
Flow cell
Nut
18-1120-06_AC.qxd 1999-04-14 14:16 Sida 3 (1,1)